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Article: Antibody reactivity of conformational peptide mimics of a conserved H5N1 neutralization site in different fusion proteins

TitleAntibody reactivity of conformational peptide mimics of a conserved H5N1 neutralization site in different fusion proteins
Authors
Issue Date2010
PublisherSpringer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705
Citation
Archives Of Virology, 2010, v. 155 n. 1, p. 19-26 How to Cite?
AbstractSeveral peptide mimics of a conserved H5N1 avian influenza virus neutralization site recognized by 8H5 mAb have been reported previously. In this study, the secondary and possibly higher structural orders of the peptide mimics 122 and 125 were investigated and found to be closely related to the specific binding with 8H5 mAb. These two peptide mimics were fused to three different carrier proteins, and the antibody binding activities were recovered in 4 of the 11 fusion proteins. HEV structural protein p239 and HBc were more suitable than the outer membrane protein T47 of the Treponema pallidum particle for the recovery of reactivity. The increase in the copy number of peptide mimics was important for the recovery of antibody-binding activity and the interaction between peptide and carrier protein may affect the spatial structure of both the peptide and the carrier protein. These results are likely to be of relevance for conformational peptide mimics in diagnostic tests, vaccine and inhibitors. © Springer-Verlag 2009.
Persistent Identifierhttp://hdl.handle.net/10722/125139
ISSN
2014 Impact Factor: 2.390
2014 SCImago Journal Rankings: 0.945
ISI Accession Number ID
Funding AgencyGrant Number
Science and Technology Foundation of Fujian Province2008Y0059
F2006BAI01B06
Chinese Ministry of Education108157
Ministry of Science and Technology2005DC105006
Chinese Ministry of Public Health2008ZX10004-006
Funding Information:

We acknowledge grants from the Science and Technology Foundation of Fujian Province ( Grant No. 2008Y0059, F2006BAI01B06), the Key Project of Chinese Ministry of Education ( Grant No. 108157), the Foundation from the Ministry of Science and Technology ( Grant No. 2005DC105006), and the Key Project of the Chinese Ministry of Public Health ( Grant No. 2008ZX10004-006). We thank Dr. Anthony ET Yeo for critical review of this manuscript.

References

 

DC FieldValueLanguage
dc.contributor.authorZheng, Zen_HK
dc.contributor.authorLuo, Wen_HK
dc.contributor.authorSong, Hen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorXiong, Jen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorGu, Yen_HK
dc.contributor.authorChen, Hen_HK
dc.contributor.authorShih, JWKen_HK
dc.contributor.authorZhang, Jen_HK
dc.contributor.authorXia, Nen_HK
dc.date.accessioned2010-10-31T11:13:36Z-
dc.date.available2010-10-31T11:13:36Z-
dc.date.issued2010en_HK
dc.identifier.citationArchives Of Virology, 2010, v. 155 n. 1, p. 19-26en_HK
dc.identifier.issn0304-8608en_HK
dc.identifier.urihttp://hdl.handle.net/10722/125139-
dc.description.abstractSeveral peptide mimics of a conserved H5N1 avian influenza virus neutralization site recognized by 8H5 mAb have been reported previously. In this study, the secondary and possibly higher structural orders of the peptide mimics 122 and 125 were investigated and found to be closely related to the specific binding with 8H5 mAb. These two peptide mimics were fused to three different carrier proteins, and the antibody binding activities were recovered in 4 of the 11 fusion proteins. HEV structural protein p239 and HBc were more suitable than the outer membrane protein T47 of the Treponema pallidum particle for the recovery of reactivity. The increase in the copy number of peptide mimics was important for the recovery of antibody-binding activity and the interaction between peptide and carrier protein may affect the spatial structure of both the peptide and the carrier protein. These results are likely to be of relevance for conformational peptide mimics in diagnostic tests, vaccine and inhibitors. © Springer-Verlag 2009.en_HK
dc.languageengen_HK
dc.publisherSpringer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705en_HK
dc.relation.ispartofArchives of Virologyen_HK
dc.subject.meshAntibodies, Monoclonal - immunologyen_HK
dc.subject.meshBinding Sites, Antibodyen_HK
dc.subject.meshInfluenza A Virus, H5N1 Subtype - chemistry - genetics - immunologyen_HK
dc.subject.meshNeutralization Testsen_HK
dc.subject.meshPeptides - chemistry - genetics - immunologyen_HK
dc.subject.meshRecombinant Fusion Proteins - chemistry - genetics - immunologyen_HK
dc.titleAntibody reactivity of conformational peptide mimics of a conserved H5N1 neutralization site in different fusion proteinsen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, H:hlchen@hkucc.hku.hken_HK
dc.identifier.authorityChen, H=rp00383en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00705-009-0542-2en_HK
dc.identifier.pmid19911251-
dc.identifier.scopuseid_2-s2.0-75049083912en_HK
dc.identifier.hkuros180632en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-75049083912&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume155en_HK
dc.identifier.issue1en_HK
dc.identifier.spage19en_HK
dc.identifier.epage26en_HK
dc.identifier.isiWOS:000273588000005-
dc.publisher.placeAustriaen_HK
dc.identifier.scopusauthoridZheng, Z=23972049100en_HK
dc.identifier.scopusauthoridLuo, W=7202199022en_HK
dc.identifier.scopusauthoridSong, H=36148613900en_HK
dc.identifier.scopusauthoridChen, Y=8705548300en_HK
dc.identifier.scopusauthoridXiong, J=22837191400en_HK
dc.identifier.scopusauthoridChen, Y=10739973700en_HK
dc.identifier.scopusauthoridGu, Y=41761534300en_HK
dc.identifier.scopusauthoridChen, H=26643315400en_HK
dc.identifier.scopusauthoridShih, JWK=7201812989en_HK
dc.identifier.scopusauthoridZhang, J=35202802400en_HK
dc.identifier.scopusauthoridXia, N=35187953700en_HK
dc.identifier.citeulike6186383-

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