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Article: Antibody reactivity of conformational peptide mimics of a conserved H5N1 neutralization site in different fusion proteins
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TitleAntibody reactivity of conformational peptide mimics of a conserved H5N1 neutralization site in different fusion proteins
 
AuthorsZheng, Z1
Luo, W1
Song, H1
Chen, Y1
Xiong, J1
Chen, Y1
Gu, Y1
Chen, H1
Shih, JWK1
Zhang, J1
Xia, N1
 
Issue Date2010
 
PublisherSpringer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705
 
CitationArchives Of Virology, 2010, v. 155 n. 1, p. 19-26 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00705-009-0542-2
 
AbstractSeveral peptide mimics of a conserved H5N1 avian influenza virus neutralization site recognized by 8H5 mAb have been reported previously. In this study, the secondary and possibly higher structural orders of the peptide mimics 122 and 125 were investigated and found to be closely related to the specific binding with 8H5 mAb. These two peptide mimics were fused to three different carrier proteins, and the antibody binding activities were recovered in 4 of the 11 fusion proteins. HEV structural protein p239 and HBc were more suitable than the outer membrane protein T47 of the Treponema pallidum particle for the recovery of reactivity. The increase in the copy number of peptide mimics was important for the recovery of antibody-binding activity and the interaction between peptide and carrier protein may affect the spatial structure of both the peptide and the carrier protein. These results are likely to be of relevance for conformational peptide mimics in diagnostic tests, vaccine and inhibitors. © Springer-Verlag 2009.
 
ISSN0304-8608
2012 Impact Factor: 2.03
2012 SCImago Journal Rankings: 0.755
 
DOIhttp://dx.doi.org/10.1007/s00705-009-0542-2
 
ISI Accession Number IDWOS:000273588000005
Funding AgencyGrant Number
Science and Technology Foundation of Fujian Province2008Y0059
F2006BAI01B06
Chinese Ministry of Education108157
Ministry of Science and Technology2005DC105006
Chinese Ministry of Public Health2008ZX10004-006
Funding Information:

We acknowledge grants from the Science and Technology Foundation of Fujian Province ( Grant No. 2008Y0059, F2006BAI01B06), the Key Project of Chinese Ministry of Education ( Grant No. 108157), the Foundation from the Ministry of Science and Technology ( Grant No. 2005DC105006), and the Key Project of the Chinese Ministry of Public Health ( Grant No. 2008ZX10004-006). We thank Dr. Anthony ET Yeo for critical review of this manuscript.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorZheng, Z
 
dc.contributor.authorLuo, W
 
dc.contributor.authorSong, H
 
dc.contributor.authorChen, Y
 
dc.contributor.authorXiong, J
 
dc.contributor.authorChen, Y
 
dc.contributor.authorGu, Y
 
dc.contributor.authorChen, H
 
dc.contributor.authorShih, JWK
 
dc.contributor.authorZhang, J
 
dc.contributor.authorXia, N
 
dc.date.accessioned2010-10-31T11:13:36Z
 
dc.date.available2010-10-31T11:13:36Z
 
dc.date.issued2010
 
dc.description.abstractSeveral peptide mimics of a conserved H5N1 avian influenza virus neutralization site recognized by 8H5 mAb have been reported previously. In this study, the secondary and possibly higher structural orders of the peptide mimics 122 and 125 were investigated and found to be closely related to the specific binding with 8H5 mAb. These two peptide mimics were fused to three different carrier proteins, and the antibody binding activities were recovered in 4 of the 11 fusion proteins. HEV structural protein p239 and HBc were more suitable than the outer membrane protein T47 of the Treponema pallidum particle for the recovery of reactivity. The increase in the copy number of peptide mimics was important for the recovery of antibody-binding activity and the interaction between peptide and carrier protein may affect the spatial structure of both the peptide and the carrier protein. These results are likely to be of relevance for conformational peptide mimics in diagnostic tests, vaccine and inhibitors. © Springer-Verlag 2009.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationArchives Of Virology, 2010, v. 155 n. 1, p. 19-26 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00705-009-0542-2
 
dc.identifier.citeulike6186383
 
dc.identifier.doihttp://dx.doi.org/10.1007/s00705-009-0542-2
 
dc.identifier.epage26
 
dc.identifier.hkuros180632
 
dc.identifier.isiWOS:000273588000005
Funding AgencyGrant Number
Science and Technology Foundation of Fujian Province2008Y0059
F2006BAI01B06
Chinese Ministry of Education108157
Ministry of Science and Technology2005DC105006
Chinese Ministry of Public Health2008ZX10004-006
Funding Information:

We acknowledge grants from the Science and Technology Foundation of Fujian Province ( Grant No. 2008Y0059, F2006BAI01B06), the Key Project of Chinese Ministry of Education ( Grant No. 108157), the Foundation from the Ministry of Science and Technology ( Grant No. 2005DC105006), and the Key Project of the Chinese Ministry of Public Health ( Grant No. 2008ZX10004-006). We thank Dr. Anthony ET Yeo for critical review of this manuscript.

 
dc.identifier.issn0304-8608
2012 Impact Factor: 2.03
2012 SCImago Journal Rankings: 0.755
 
dc.identifier.issue1
 
dc.identifier.pmid19911251
 
dc.identifier.scopuseid_2-s2.0-75049083912
 
dc.identifier.spage19
 
dc.identifier.urihttp://hdl.handle.net/10722/125139
 
dc.identifier.volume155
 
dc.languageeng
 
dc.publisherSpringer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705
 
dc.publisher.placeAustria
 
dc.relation.ispartofArchives of Virology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAntibodies, Monoclonal - immunology
 
dc.subject.meshBinding Sites, Antibody
 
dc.subject.meshInfluenza A Virus, H5N1 Subtype - chemistry - genetics - immunology
 
dc.subject.meshNeutralization Tests
 
dc.subject.meshPeptides - chemistry - genetics - immunology
 
dc.subject.meshRecombinant Fusion Proteins - chemistry - genetics - immunology
 
dc.titleAntibody reactivity of conformational peptide mimics of a conserved H5N1 neutralization site in different fusion proteins
 
dc.typeArticle
 
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Author Affiliations
  1. Xiamen University