File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: An M2e-based multiple antigenic peptide vaccine protects mice from lethal challenge with divergent H5N1 influenza viruses

TitleAn M2e-based multiple antigenic peptide vaccine protects mice from lethal challenge with divergent H5N1 influenza viruses
Authors
Issue Date2010
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.virologyj.com/home/
Citation
Virology Journal, 2010, v. 7 How to Cite?
AbstractBackground. A growing concern has raised regarding the pandemic potential of the highly pathogenic avian influenza (HPAI) H5N1 viruses. Consequently, there is an urgent need to develop an effective and safe vaccine against the divergent H5N1 influenza viruses. In the present study, we designed a tetra-branched multiple antigenic peptide (MAP)-based vaccine, designated M2e-MAP, which contains the sequence overlapping the highly conserved extracellular domain of matrix protein 2 (M2e) of a HPAI H5N1 virus, and investigated its immune responses and cross-protection against different clades of H5N1 viruses. Results. Our results showed that M2e-MAP vaccine induced strong M2e-specific IgG antibody responses following 3-dose immunization of mice with M2e-MAP in the presence of Freunds' or aluminium (alum) adjuvant. M2e-MAP vaccination limited viral replication and attenuated histopathological damage in the challenged mouse lungs. The M2e-MAP-based vaccine protected immunized mice against both clade1: VN/1194 and clade2.3.4: SZ/406H H5N1 virus challenge, being able to counteract weight lost and elevate survival rate following lethal challenge of H5N1 viruses. Conclusions. These results suggest that M2e-MAP presenting M2e of H5N1 virus has a great potential to be developed into an effective subunit vaccine for the prevention of infection by a broad spectrum of HPAI H5N1 viruses. © 2010 Zhao et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/125131
ISSN
2014 Impact Factor: 2.181
2014 SCImago Journal Rankings: 0.905
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
National High Technology R&D Program of China2006AA02Z406
National Basic Research Program of China2005CB523001
National Natural Science Foundation of China30901371
Mega-projects of Science Research2009ZX10004-4001
University Grants CommitteeAoE/M-12/06
Research Fund for the Control of Infectious Diseases, Hong Kong SAR09080812
Funding Information:

This study was supported by the National High Technology R&D Program of China (863 Program, No. 2006AA02Z406), National Basic Research Program of China (973 Program, No. 2005CB523001), National Natural Science Foundation of China (30901371), Mega-projects of Science Research for the 11th Five-Year Plan (2009ZX10004-4001), the Area of Excellence Scheme of the University Grants Committee (Grant AoE/M-12/06) and Research Fund for the Control of Infectious Diseases (09080812), Hong Kong SAR.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorZhao, Gen_HK
dc.contributor.authorLin, Yen_HK
dc.contributor.authorDu, Len_HK
dc.contributor.authorGuan, Jen_HK
dc.contributor.authorSun, Sen_HK
dc.contributor.authorSui, Hen_HK
dc.contributor.authorKou, Zen_HK
dc.contributor.authorChan, CCen_HK
dc.contributor.authorGuo, Yen_HK
dc.contributor.authorJiang, Sen_HK
dc.contributor.authorZheng, BJen_HK
dc.contributor.authorZhou, Yen_HK
dc.date.accessioned2010-10-31T11:13:09Z-
dc.date.available2010-10-31T11:13:09Z-
dc.date.issued2010en_HK
dc.identifier.citationVirology Journal, 2010, v. 7en_HK
dc.identifier.issn1743-422Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/125131-
dc.description.abstractBackground. A growing concern has raised regarding the pandemic potential of the highly pathogenic avian influenza (HPAI) H5N1 viruses. Consequently, there is an urgent need to develop an effective and safe vaccine against the divergent H5N1 influenza viruses. In the present study, we designed a tetra-branched multiple antigenic peptide (MAP)-based vaccine, designated M2e-MAP, which contains the sequence overlapping the highly conserved extracellular domain of matrix protein 2 (M2e) of a HPAI H5N1 virus, and investigated its immune responses and cross-protection against different clades of H5N1 viruses. Results. Our results showed that M2e-MAP vaccine induced strong M2e-specific IgG antibody responses following 3-dose immunization of mice with M2e-MAP in the presence of Freunds' or aluminium (alum) adjuvant. M2e-MAP vaccination limited viral replication and attenuated histopathological damage in the challenged mouse lungs. The M2e-MAP-based vaccine protected immunized mice against both clade1: VN/1194 and clade2.3.4: SZ/406H H5N1 virus challenge, being able to counteract weight lost and elevate survival rate following lethal challenge of H5N1 viruses. Conclusions. These results suggest that M2e-MAP presenting M2e of H5N1 virus has a great potential to be developed into an effective subunit vaccine for the prevention of infection by a broad spectrum of HPAI H5N1 viruses. © 2010 Zhao et al; licensee BioMed Central Ltd.en_HK
dc.languageengen_HK
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.virologyj.com/home/en_HK
dc.relation.ispartofVirology Journalen_HK
dc.rightsVirology Journal. Copyright © BioMed Central Ltd.-
dc.subject.meshAdjuvants, Immunologic - administration and dosage-
dc.subject.meshAntigens, Viral - immunology-
dc.subject.meshInfluenza A Virus, H5N1 Subtype - immunology-
dc.subject.meshInfluenza Vaccines - immunology-
dc.subject.meshViral Matrix Proteins - immunology-
dc.titleAn M2e-based multiple antigenic peptide vaccine protects mice from lethal challenge with divergent H5N1 influenza virusesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1743-422X&volume=7&issue=article no. 9&spage=&epage=&date=2010&atitle=An+M2e-based+multiple+antigenic+peptide+vaccine+protects+mice+from+lethal+challenge+with+divergent+H5N1+influenza+viruses-
dc.identifier.emailZheng, BJ:bzheng@hkucc.hku.hken_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1743-422X-7-9en_HK
dc.identifier.pmid20082709en_HK
dc.identifier.pmcidPMC2823673-
dc.identifier.scopuseid_2-s2.0-77249104563en_HK
dc.identifier.hkuros175099en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77249104563&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issuearticle no. 9en_HK
dc.identifier.eissn1743-422X-
dc.identifier.isiWOS:000275091300001-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectCross-protective efficacy of immunization with different forms of M2 vaccines and their combinations with HA vaccines against highly pathogenic H5N1 influenza A viruses in mice-
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza-
dc.identifier.scopusauthoridZhao, G=8684553000en_HK
dc.identifier.scopusauthoridLin, Y=8591935100en_HK
dc.identifier.scopusauthoridDu, L=8686996200en_HK
dc.identifier.scopusauthoridGuan, J=35573273400en_HK
dc.identifier.scopusauthoridSun, S=35171536200en_HK
dc.identifier.scopusauthoridSui, H=23971615600en_HK
dc.identifier.scopusauthoridKou, Z=23034818200en_HK
dc.identifier.scopusauthoridChan, CC=36984588300en_HK
dc.identifier.scopusauthoridGuo, Y=8555122500en_HK
dc.identifier.scopusauthoridJiang, S=7404453146en_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK
dc.identifier.scopusauthoridZhou, Y=8791655300en_HK
dc.identifier.citeulike6564277-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats