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Article: A rapid test for the detection of influenza A virus including pandemic influenza A/H1N1 2009

TitleA rapid test for the detection of influenza A virus including pandemic influenza A/H1N1 2009
Authors
KeywordsDot-ELISA
Influenza A
Nucleoprotein
Rapid test
Issue Date2010
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jviromet
Citation
Journal Of Virological Methods, 2010, v. 167 n. 1, p. 100-102 How to Cite?
Abstract
A new rapid diagnostic test for detection of influenza A virus was evaluated with four sets of experiments: first, a comparison with a commercial diagnostic kit against a panel of virus strains was conducted; second, the kit was tested against a collection of 40 strains of influenza A virus isolated from five different host species and 26 strains of other respiratory viruses used as controls; third, the kit was tested against specimens collected in the field obtained from human and chicken; and fourth, the kit was tested against the novel pandemic influenza A/H1N1 2009 clinical specimens obtained from admitted to hospital patients. The test kit displayed a sensitivity of 88% for both human specimens and avian specimens. The corresponding specificity was 99.3% for human specimens and 96.5% for avian specimens. This test kit may be useful for rapid diagnosis of influenza A virus. © 2010.
Persistent Identifierhttp://hdl.handle.net/10722/125130
ISSN
2013 Impact Factor: 1.883
2013 SCImago Journal Rankings: 0.860
ISI Accession Number ID
Funding AgencyGrant Number
National Natural Science Foundation of China30901077
Important National Science & Technology Specific Projects2008ZX10004-006
Project of Fujian Province2008Y0059
2009YZ0002
Funding Information:

We thank Prof. James Wai-Kuo Shih for helpful discussions and comments in this manuscript. This study was supported by National Natural Science Foundation of China (30901077); Important National Science & Technology Specific Projects (2008ZX10004-006); the Project of Fujian Province (2008Y0059 & 2009YZ0002).

References

 

DC FieldValueLanguage
dc.contributor.authorChen, Yen_HK
dc.contributor.authorXu, Fen_HK
dc.contributor.authorGui, Xen_HK
dc.contributor.authorYang, Ken_HK
dc.contributor.authorWu, Xen_HK
dc.contributor.authorZheng, Qen_HK
dc.contributor.authorGe, Sen_HK
dc.contributor.authorYuan, Qen_HK
dc.contributor.authorYeo, AETen_HK
dc.contributor.authorZhang, Jen_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorChen, Hen_HK
dc.contributor.authorXia, Nen_HK
dc.date.accessioned2010-10-31T11:13:05Z-
dc.date.available2010-10-31T11:13:05Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Virological Methods, 2010, v. 167 n. 1, p. 100-102en_HK
dc.identifier.issn0166-0934en_HK
dc.identifier.urihttp://hdl.handle.net/10722/125130-
dc.description.abstractA new rapid diagnostic test for detection of influenza A virus was evaluated with four sets of experiments: first, a comparison with a commercial diagnostic kit against a panel of virus strains was conducted; second, the kit was tested against a collection of 40 strains of influenza A virus isolated from five different host species and 26 strains of other respiratory viruses used as controls; third, the kit was tested against specimens collected in the field obtained from human and chicken; and fourth, the kit was tested against the novel pandemic influenza A/H1N1 2009 clinical specimens obtained from admitted to hospital patients. The test kit displayed a sensitivity of 88% for both human specimens and avian specimens. The corresponding specificity was 99.3% for human specimens and 96.5% for avian specimens. This test kit may be useful for rapid diagnosis of influenza A virus. © 2010.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jvirometen_HK
dc.relation.ispartofJournal of Virological Methodsen_HK
dc.subjectDot-ELISAen_HK
dc.subjectInfluenza Aen_HK
dc.subjectNucleoproteinen_HK
dc.subjectRapid testen_HK
dc.subject.meshInfluenza A virus - classification - isolation and purification-
dc.subject.meshInfluenza in Birds - diagnosis - virology-
dc.subject.meshInfluenza, Human - diagnosis - virology-
dc.subject.meshPoultry Diseases - diagnosis - virology-
dc.subject.meshVirology - methods-
dc.titleA rapid test for the detection of influenza A virus including pandemic influenza A/H1N1 2009en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0166-0934&volume=167&issue=1&spage=100&epage=102&date=2010&atitle=A+rapid+test+for+the+detection+of+influenza+A+virus+including+pandemic+influenza+A/H1N1+2009-
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.emailChen, H: hlchen@hku.hken_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.identifier.authorityChen, H=rp00383en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jviromet.2010.02.001en_HK
dc.identifier.pmid20144656en_HK
dc.identifier.scopuseid_2-s2.0-77953021694en_HK
dc.identifier.hkuros180280en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77953021694&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume167en_HK
dc.identifier.issue1en_HK
dc.identifier.spage100en_HK
dc.identifier.epage102en_HK
dc.identifier.isiWOS:000278782700017-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChen, Y=10739973700en_HK
dc.identifier.scopusauthoridXu, F=35202363800en_HK
dc.identifier.scopusauthoridGui, X=36092830100en_HK
dc.identifier.scopusauthoridYang, K=8285362900en_HK
dc.identifier.scopusauthoridWu, X=36447130600en_HK
dc.identifier.scopusauthoridZheng, Q=35202845100en_HK
dc.identifier.scopusauthoridGe, S=35227303000en_HK
dc.identifier.scopusauthoridYuan, Q=36060907700en_HK
dc.identifier.scopusauthoridYeo, AET=35723458400en_HK
dc.identifier.scopusauthoridZhang, J=35202802400en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridChen, H=26643315400en_HK
dc.identifier.scopusauthoridXia, N=35187953700en_HK
dc.identifier.citeulike6781560-

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