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Article: A rapid test for the detection of influenza A virus including pandemic influenza A/H1N1 2009
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TitleA rapid test for the detection of influenza A virus including pandemic influenza A/H1N1 2009
 
AuthorsChen, Y1
Xu, F1
Gui, X1
Yang, K1
Wu, X1
Zheng, Q1
Ge, S1
Yuan, Q1
Yeo, AET1
Zhang, J1
Guan, Y2
Chen, H2
Xia, N1
 
KeywordsDot-ELISA
Influenza A
Nucleoprotein
Rapid test
 
Issue Date2010
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jviromet
 
CitationJournal Of Virological Methods, 2010, v. 167 n. 1, p. 100-102 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jviromet.2010.02.001
 
AbstractA new rapid diagnostic test for detection of influenza A virus was evaluated with four sets of experiments: first, a comparison with a commercial diagnostic kit against a panel of virus strains was conducted; second, the kit was tested against a collection of 40 strains of influenza A virus isolated from five different host species and 26 strains of other respiratory viruses used as controls; third, the kit was tested against specimens collected in the field obtained from human and chicken; and fourth, the kit was tested against the novel pandemic influenza A/H1N1 2009 clinical specimens obtained from admitted to hospital patients. The test kit displayed a sensitivity of 88% for both human specimens and avian specimens. The corresponding specificity was 99.3% for human specimens and 96.5% for avian specimens. This test kit may be useful for rapid diagnosis of influenza A virus. © 2010.
 
ISSN0166-0934
2013 Impact Factor: 1.883
2013 SCImago Journal Rankings: 0.860
 
DOIhttp://dx.doi.org/10.1016/j.jviromet.2010.02.001
 
ISI Accession Number IDWOS:000278782700017
Funding AgencyGrant Number
National Natural Science Foundation of China30901077
Important National Science & Technology Specific Projects2008ZX10004-006
Project of Fujian Province2008Y0059
2009YZ0002
Funding Information:

We thank Prof. James Wai-Kuo Shih for helpful discussions and comments in this manuscript. This study was supported by National Natural Science Foundation of China (30901077); Important National Science & Technology Specific Projects (2008ZX10004-006); the Project of Fujian Province (2008Y0059 & 2009YZ0002).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChen, Y
 
dc.contributor.authorXu, F
 
dc.contributor.authorGui, X
 
dc.contributor.authorYang, K
 
dc.contributor.authorWu, X
 
dc.contributor.authorZheng, Q
 
dc.contributor.authorGe, S
 
dc.contributor.authorYuan, Q
 
dc.contributor.authorYeo, AET
 
dc.contributor.authorZhang, J
 
dc.contributor.authorGuan, Y
 
dc.contributor.authorChen, H
 
dc.contributor.authorXia, N
 
dc.date.accessioned2010-10-31T11:13:05Z
 
dc.date.available2010-10-31T11:13:05Z
 
dc.date.issued2010
 
dc.description.abstractA new rapid diagnostic test for detection of influenza A virus was evaluated with four sets of experiments: first, a comparison with a commercial diagnostic kit against a panel of virus strains was conducted; second, the kit was tested against a collection of 40 strains of influenza A virus isolated from five different host species and 26 strains of other respiratory viruses used as controls; third, the kit was tested against specimens collected in the field obtained from human and chicken; and fourth, the kit was tested against the novel pandemic influenza A/H1N1 2009 clinical specimens obtained from admitted to hospital patients. The test kit displayed a sensitivity of 88% for both human specimens and avian specimens. The corresponding specificity was 99.3% for human specimens and 96.5% for avian specimens. This test kit may be useful for rapid diagnosis of influenza A virus. © 2010.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Virological Methods, 2010, v. 167 n. 1, p. 100-102 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jviromet.2010.02.001
 
dc.identifier.citeulike6781560
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.jviromet.2010.02.001
 
dc.identifier.epage102
 
dc.identifier.hkuros180280
 
dc.identifier.isiWOS:000278782700017
Funding AgencyGrant Number
National Natural Science Foundation of China30901077
Important National Science & Technology Specific Projects2008ZX10004-006
Project of Fujian Province2008Y0059
2009YZ0002
Funding Information:

We thank Prof. James Wai-Kuo Shih for helpful discussions and comments in this manuscript. This study was supported by National Natural Science Foundation of China (30901077); Important National Science & Technology Specific Projects (2008ZX10004-006); the Project of Fujian Province (2008Y0059 & 2009YZ0002).

 
dc.identifier.issn0166-0934
2013 Impact Factor: 1.883
2013 SCImago Journal Rankings: 0.860
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.pmid20144656
 
dc.identifier.scopuseid_2-s2.0-77953021694
 
dc.identifier.spage100
 
dc.identifier.urihttp://hdl.handle.net/10722/125130
 
dc.identifier.volume167
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jviromet
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofJournal of Virological Methods
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshInfluenza A virus - classification - isolation and purification
 
dc.subject.meshInfluenza in Birds - diagnosis - virology
 
dc.subject.meshInfluenza, Human - diagnosis - virology
 
dc.subject.meshPoultry Diseases - diagnosis - virology
 
dc.subject.meshVirology - methods
 
dc.subjectDot-ELISA
 
dc.subjectInfluenza A
 
dc.subjectNucleoprotein
 
dc.subjectRapid test
 
dc.titleA rapid test for the detection of influenza A virus including pandemic influenza A/H1N1 2009
 
dc.typeArticle
 
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Author Affiliations
  1. Xiamen University
  2. The University of Hong Kong