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Article: Antigenicity and immunogenicity of SARS-CoV S protein receptor-binding domain stably expressed in CHO cells

TitleAntigenicity and immunogenicity of SARS-CoV S protein receptor-binding domain stably expressed in CHO cells
Authors
KeywordsAntigenicity
CHO cells
Immunogenicity
Receptor-binding domain
S protein
SARS-CoV
Stable expression
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2009, v. 384 n. 4, p. 486-490 How to Cite?
AbstractThe receptor-binding domain (RBD) of SARS coronavirus (SARS-CoV) spike (S) protein contains multiple conformation-dependent epitopes that induce neutralizing antibody responses. Here we used CHO-K1 cells to establish a cell line for stable expression of a 193-mer (residues 318-510) RBD (RBD193-CHO) and determined its antigenicity and immunogenicity. We found that RBD193-CHO reacted strongly with a panel of six monoclonal antibodies recognizing various conformational and linear epitopes in RBD, suggesting that this recombinant protein maintains intact conformation and good antigenicity. Immunization of mice with RBD193-CHO resulted in induction of high titers of RBD-specific neutralizing antibodies and potent IL-4-expressing T cell responses. RBD193-CHO induced immunity that protected a majority of the vaccinated mice from SARS-CoV challenge. These results suggest that the recombinant RBD produced in an established stable cell line maintains strong immunogenicity with high potential for use as an effective and economic subunit SARS vaccine. © 2009 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/125119
ISSN
2014 Impact Factor: 2.297
2014 SCImago Journal Rankings: 1.006
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDu, Len_HK
dc.contributor.authorZhao, Gen_HK
dc.contributor.authorLi, Len_HK
dc.contributor.authorHe, Yen_HK
dc.contributor.authorZhou, Yen_HK
dc.contributor.authorZheng, BJen_HK
dc.contributor.authorJiang, Sen_HK
dc.date.accessioned2010-10-31T11:12:28Z-
dc.date.available2010-10-31T11:12:28Z-
dc.date.issued2009en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2009, v. 384 n. 4, p. 486-490en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/125119-
dc.description.abstractThe receptor-binding domain (RBD) of SARS coronavirus (SARS-CoV) spike (S) protein contains multiple conformation-dependent epitopes that induce neutralizing antibody responses. Here we used CHO-K1 cells to establish a cell line for stable expression of a 193-mer (residues 318-510) RBD (RBD193-CHO) and determined its antigenicity and immunogenicity. We found that RBD193-CHO reacted strongly with a panel of six monoclonal antibodies recognizing various conformational and linear epitopes in RBD, suggesting that this recombinant protein maintains intact conformation and good antigenicity. Immunization of mice with RBD193-CHO resulted in induction of high titers of RBD-specific neutralizing antibodies and potent IL-4-expressing T cell responses. RBD193-CHO induced immunity that protected a majority of the vaccinated mice from SARS-CoV challenge. These results suggest that the recombinant RBD produced in an established stable cell line maintains strong immunogenicity with high potential for use as an effective and economic subunit SARS vaccine. © 2009 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectAntigenicityen_HK
dc.subjectCHO cellsen_HK
dc.subjectImmunogenicityen_HK
dc.subjectReceptor-binding domainen_HK
dc.subjectS proteinen_HK
dc.subjectSARS-CoVen_HK
dc.subjectStable expressionen_HK
dc.subject.meshAnimals-
dc.subject.meshAntibodies, Viral - biosynthesis-
dc.subject.meshMembrane Glycoproteins - biosynthesis - immunology-
dc.subject.meshSARS Virus - immunology-
dc.subject.meshViral Envelope Proteins - biosynthesis - immunology-
dc.titleAntigenicity and immunogenicity of SARS-CoV S protein receptor-binding domain stably expressed in CHO cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=384&issue=4&spage=486&epage=490&date=2009&atitle=Antigenicity+and+immunogenicity+of+SARS-CoV+S+protein+receptor-binding+domain+stably+expressed+in+CHO+cells.-
dc.identifier.emailZheng, BJ:bzheng@hkucc.hku.hken_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.bbrc.2009.05.003en_HK
dc.identifier.pmid19422787en_HK
dc.identifier.pmcidPMC2750803-
dc.identifier.scopuseid_2-s2.0-65649110128en_HK
dc.identifier.hkuros175095en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-65649110128&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume384en_HK
dc.identifier.issue4en_HK
dc.identifier.spage486en_HK
dc.identifier.epage490en_HK
dc.identifier.isiWOS:000266689300017-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridDu, L=8686996200en_HK
dc.identifier.scopusauthoridZhao, G=8684553000en_HK
dc.identifier.scopusauthoridLi, L=36072593200en_HK
dc.identifier.scopusauthoridHe, Y=8742157400en_HK
dc.identifier.scopusauthoridZhou, Y=8791655300en_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK
dc.identifier.scopusauthoridJiang, S=7404453146en_HK

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