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Article: Mutations in influenza virus replication and transcription: Detection of amino acid substitutions in hemagglutinin of an avian influenza virus (H1N1)

TitleMutations in influenza virus replication and transcription: Detection of amino acid substitutions in hemagglutinin of an avian influenza virus (H1N1)
Authors
KeywordsInfection
Mass spectrometric sequencing
Mutation
Polymorphism of viral antigen
Receptor
Replication and transcription
Issue Date2009
PublisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/
Citation
FASEB Journal, 2009, v. 23 n. 10, p. 3377-3382 How to Cite?
AbstractInfluenza A viruses are RNA viruses that contain negative-sense, single-stranded, and segmented RNA genome, which depends on virally encoded RNAdependent RNA polymerase and cellular DNA-dependent RNA polymerase for replication of viral genome and transcription of viral mRNA, respectively. Hemagglutinin (HA), one of the major surface proteins of the influenza virus, is responsible for virus attachment to the receptor of host cells to initiate an infection. Amino acid (AA) substitutions in HA may cause changes in virus antigenicity and even receptor specificity. To detect the AA substitutions within HA at protein level, nanoelectrospray-MS/MS was used to analyze tryptic digestion of HA antigen directly purified from virus particles of an avian influenza virus, A/WDK/JX/12416/2005 (H1N1), of which the HA gene was sequenced as a reference. The comparison of the sequences obtained from analysis of viral genome and peptide found seven variations between HA gene and protein, namely E103K, R130K, T169I, I338V, N387S, S398I/L, and I399S in HA. Because influenza virus uses different polymerase machineries for replication and transcription, these substitutions could be introduced in the viral genome through replication process but not in viral mRNA in the transcription. The results, for the first time, provided experimental evidence showing differences in AA sequence obtained from direct analysis of viral protein derived from viral genome. © FASEB.
Persistent Identifierhttp://hdl.handle.net/10722/125114
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council of the Hong Kong SARHKU 7500/06M
HKU 7488/05M
Area of Excellence Scheme of the UniversityAoE/M-12/06
research fund for the control of infectious diseases, Hong Kong SAR
Li Ka Shing Foundation
Hong Kong Baptist UniversityFRG/07-08/II-21
Funding Information:

This work was supported by the Research Grants Council of the Hong Kong SAR (HKU 7500/06M and HKU 7488/05M); the Area of Excellence Scheme of the University Grants Committee (AoE/M-12/06); the research fund for the control of infectious diseases, Hong Kong SAR; the Li Ka Shing Foundation; and a faculty research grant from Hong Kong Baptist University (FRG/07-08/II-21).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorLiu, Nen_HK
dc.contributor.authorWang, Gen_HK
dc.contributor.authorKim, CLen_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorChen, Hen_HK
dc.contributor.authorCai, Zen_HK
dc.date.accessioned2010-10-31T11:12:10Z-
dc.date.available2010-10-31T11:12:10Z-
dc.date.issued2009en_HK
dc.identifier.citationFASEB Journal, 2009, v. 23 n. 10, p. 3377-3382en_HK
dc.identifier.issn0892-6638en_HK
dc.identifier.urihttp://hdl.handle.net/10722/125114-
dc.description.abstractInfluenza A viruses are RNA viruses that contain negative-sense, single-stranded, and segmented RNA genome, which depends on virally encoded RNAdependent RNA polymerase and cellular DNA-dependent RNA polymerase for replication of viral genome and transcription of viral mRNA, respectively. Hemagglutinin (HA), one of the major surface proteins of the influenza virus, is responsible for virus attachment to the receptor of host cells to initiate an infection. Amino acid (AA) substitutions in HA may cause changes in virus antigenicity and even receptor specificity. To detect the AA substitutions within HA at protein level, nanoelectrospray-MS/MS was used to analyze tryptic digestion of HA antigen directly purified from virus particles of an avian influenza virus, A/WDK/JX/12416/2005 (H1N1), of which the HA gene was sequenced as a reference. The comparison of the sequences obtained from analysis of viral genome and peptide found seven variations between HA gene and protein, namely E103K, R130K, T169I, I338V, N387S, S398I/L, and I399S in HA. Because influenza virus uses different polymerase machineries for replication and transcription, these substitutions could be introduced in the viral genome through replication process but not in viral mRNA in the transcription. The results, for the first time, provided experimental evidence showing differences in AA sequence obtained from direct analysis of viral protein derived from viral genome. © FASEB.en_HK
dc.languageengen_HK
dc.publisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/en_HK
dc.relation.ispartofFASEB Journalen_HK
dc.subjectInfectionen_HK
dc.subjectMass spectrometric sequencingen_HK
dc.subjectMutationen_HK
dc.subjectPolymorphism of viral antigenen_HK
dc.subjectReceptoren_HK
dc.subjectReplication and transcriptionen_HK
dc.subject.meshAmino Acid Sequenceen_HK
dc.subject.meshAmino Acid Substitutionen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBirds - virologyen_HK
dc.subject.meshHemagglutinin Glycoproteins, Influenza Virus - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInfluenza A Virus, H1N1 Subtype - geneticsen_HK
dc.subject.meshInfluenza in Birds - virologyen_HK
dc.subject.meshInfluenza, Human - virologyen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshMutationen_HK
dc.subject.meshTranscription, Geneticen_HK
dc.subject.meshVirus Replication - geneticsen_HK
dc.titleMutations in influenza virus replication and transcription: Detection of amino acid substitutions in hemagglutinin of an avian influenza virus (H1N1)en_HK
dc.typeArticleen_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.emailChen, H: hlchen@hku.hken_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.identifier.authorityChen, H=rp00383en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1096/fj.09-134072en_HK
dc.identifier.pmid19553505en_HK
dc.identifier.scopuseid_2-s2.0-70349661240en_HK
dc.identifier.hkuros180684en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70349661240&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume23en_HK
dc.identifier.issue10en_HK
dc.identifier.spage3377en_HK
dc.identifier.epage3382en_HK
dc.identifier.isiWOS:000270354300015-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectMolecular basis of genesis and determinants for pathogenicity of avian influenza H5N1 virus genotype Z-
dc.relation.projectH5N1 virus heterogeneity and mechanisms for adaptation to new hosts-
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza-
dc.identifier.scopusauthoridLiu, N=36079934800en_HK
dc.identifier.scopusauthoridWang, G=35189761800en_HK
dc.identifier.scopusauthoridKim, CL=35189036800en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridChen, H=26643315400en_HK
dc.identifier.scopusauthoridCai, Z=7402904946en_HK
dc.identifier.citeulike5007955-

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