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Article: ABCG1 mediated oxidized LDL-derived oxysterol efflux from macrophages
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TitleABCG1 mediated oxidized LDL-derived oxysterol efflux from macrophages
 
AuthorsXu, M2
Zhou, H2
Tan, KCB1
Guo, R2
Shiu, SWM1
Wong, Y1
 
KeywordsATP binding cassette transporters
Oxidized LDL
Oxysterols
Reverse cholesterol transport
Scavenger receptor class B type I
 
Issue Date2009
 
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
 
CitationBiochemical And Biophysical Research Communications, 2009, v. 390 n. 4, p. 1349-1354 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.bbrc.2009.10.152
 
AbstractObjectives: The uptake of oxidized LDL (oxLDL) by macrophages is a key initial event in atherogenesis, and the removal of oxidized lipids from artery wall via reverse cholesterol transport is considered antiatherogenic. The aims of this study were to investigate the pathways mediating the removal of oxysterols from oxLDL-loaded macrophages, and the subsequent uptake of the oxysterols by hepatocytes. Methods: LDL was labeled with [3H]cholesterol, and LDL-[3H]cholesterol was oxidized by copper using a standard method. [3H]oxysterol formation in oxLDL was analyzed by thin layer chromatography. oxLDL-[3H]sterol was incubated with macrophages, allowing the uptake of [3H]sterol by macrophages. [3H]sterol efflux from macrophages mediated by ATP binding cassette transporters (ABCA1, ABCG1), or scavenger receptor class B type I (SR-BI) was measured. The subsequent uptake of the [3H]sterol by hepatocytes was also determined. Results: 7-Ketocholesterol was the major oxysterol formed in oxLDL, and it was significantly higher in oxLDL compared with that in native LDL (naLDL). oxLDL-derived sterol efflux to HDL from macrophages was significantly increased compared with naLDL-derived sterol, and it was mainly mediated by ABCG1, but not by ABCA1 or SR-BI. Moreover, although HDL dose-dependently induced sterol efflux from macrophages, only the exported sterol by ABCG1 pathway was efficiently taken up by hepatocytes. Conclusions: ABCG1 mediates oxysterol efflux from oxLDL-loaded macrophages, and the exported oxysterol by ABCG1 pathway can be selectively taken up by hepatocytes. © 2009 Elsevier Inc. All rights reserved.
 
ISSN0006-291X
2013 Impact Factor: 2.281
 
DOIhttp://dx.doi.org/10.1016/j.bbrc.2009.10.152
 
ISI Accession Number IDWOS:000272650800050
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorXu, M
 
dc.contributor.authorZhou, H
 
dc.contributor.authorTan, KCB
 
dc.contributor.authorGuo, R
 
dc.contributor.authorShiu, SWM
 
dc.contributor.authorWong, Y
 
dc.date.accessioned2010-10-31T11:10:32Z
 
dc.date.available2010-10-31T11:10:32Z
 
dc.date.issued2009
 
dc.description.abstractObjectives: The uptake of oxidized LDL (oxLDL) by macrophages is a key initial event in atherogenesis, and the removal of oxidized lipids from artery wall via reverse cholesterol transport is considered antiatherogenic. The aims of this study were to investigate the pathways mediating the removal of oxysterols from oxLDL-loaded macrophages, and the subsequent uptake of the oxysterols by hepatocytes. Methods: LDL was labeled with [3H]cholesterol, and LDL-[3H]cholesterol was oxidized by copper using a standard method. [3H]oxysterol formation in oxLDL was analyzed by thin layer chromatography. oxLDL-[3H]sterol was incubated with macrophages, allowing the uptake of [3H]sterol by macrophages. [3H]sterol efflux from macrophages mediated by ATP binding cassette transporters (ABCA1, ABCG1), or scavenger receptor class B type I (SR-BI) was measured. The subsequent uptake of the [3H]sterol by hepatocytes was also determined. Results: 7-Ketocholesterol was the major oxysterol formed in oxLDL, and it was significantly higher in oxLDL compared with that in native LDL (naLDL). oxLDL-derived sterol efflux to HDL from macrophages was significantly increased compared with naLDL-derived sterol, and it was mainly mediated by ABCG1, but not by ABCA1 or SR-BI. Moreover, although HDL dose-dependently induced sterol efflux from macrophages, only the exported sterol by ABCG1 pathway was efficiently taken up by hepatocytes. Conclusions: ABCG1 mediates oxysterol efflux from oxLDL-loaded macrophages, and the exported oxysterol by ABCG1 pathway can be selectively taken up by hepatocytes. © 2009 Elsevier Inc. All rights reserved.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationBiochemical And Biophysical Research Communications, 2009, v. 390 n. 4, p. 1349-1354 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.bbrc.2009.10.152
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.bbrc.2009.10.152
 
dc.identifier.epage1354
 
dc.identifier.hkuros174663
 
dc.identifier.isiWOS:000272650800050
 
dc.identifier.issn0006-291X
2013 Impact Factor: 2.281
 
dc.identifier.issue4
 
dc.identifier.openurl
 
dc.identifier.pmid19895785
 
dc.identifier.scopuseid_2-s2.0-70450263334
 
dc.identifier.spage1349
 
dc.identifier.urihttp://hdl.handle.net/10722/125085
 
dc.identifier.volume390
 
dc.languageeng
 
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
 
dc.publisher.placeUnited States
 
dc.relation.ispartofBiochemical and Biophysical Research Communications
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshATP-Binding Cassette Transporters - metabolism
 
dc.subject.meshCell Line, Tumor
 
dc.subject.meshHepatocytes - metabolism
 
dc.subject.meshLipoproteins, LDL - metabolism
 
dc.subject.meshMacrophages - metabolism
 
dc.subjectATP binding cassette transporters
 
dc.subjectOxidized LDL
 
dc.subjectOxysterols
 
dc.subjectReverse cholesterol transport
 
dc.subjectScavenger receptor class B type I
 
dc.titleABCG1 mediated oxidized LDL-derived oxysterol efflux from macrophages
 
dc.typeArticle
 
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<contributor.author>Zhou, H</contributor.author>
<contributor.author>Tan, KCB</contributor.author>
<contributor.author>Guo, R</contributor.author>
<contributor.author>Shiu, SWM</contributor.author>
<contributor.author>Wong, Y</contributor.author>
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<description.abstract>Objectives: The uptake of oxidized LDL (oxLDL) by macrophages is a key initial event in atherogenesis, and the removal of oxidized lipids from artery wall via reverse cholesterol transport is considered antiatherogenic. The aims of this study were to investigate the pathways mediating the removal of oxysterols from oxLDL-loaded macrophages, and the subsequent uptake of the oxysterols by hepatocytes. Methods: LDL was labeled with [3H]cholesterol, and LDL-[3H]cholesterol was oxidized by copper using a standard method. [3H]oxysterol formation in oxLDL was analyzed by thin layer chromatography. oxLDL-[3H]sterol was incubated with macrophages, allowing the uptake of [3H]sterol by macrophages. [3H]sterol efflux from macrophages mediated by ATP binding cassette transporters (ABCA1, ABCG1), or scavenger receptor class B type I (SR-BI) was measured. The subsequent uptake of the [3H]sterol by hepatocytes was also determined. Results: 7-Ketocholesterol was the major oxysterol formed in oxLDL, and it was significantly higher in oxLDL compared with that in native LDL (naLDL). oxLDL-derived sterol efflux to HDL from macrophages was significantly increased compared with naLDL-derived sterol, and it was mainly mediated by ABCG1, but not by ABCA1 or SR-BI. Moreover, although HDL dose-dependently induced sterol efflux from macrophages, only the exported sterol by ABCG1 pathway was efficiently taken up by hepatocytes. Conclusions: ABCG1 mediates oxysterol efflux from oxLDL-loaded macrophages, and the exported oxysterol by ABCG1 pathway can be selectively taken up by hepatocytes. &#169; 2009 Elsevier Inc. All rights reserved.</description.abstract>
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<subject>ATP binding cassette transporters</subject>
<subject>Oxidized LDL</subject>
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Author Affiliations
  1. The University of Hong Kong
  2. The First Affiliated Hospital