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Article: Defective phenotype of mesenchymal stem cells in patients with systemic lupus erythematosus

TitleDefective phenotype of mesenchymal stem cells in patients with systemic lupus erythematosus
Authors
KeywordsAutoimmune disease
Mesenchymal stem cells
Systemic lupus erythematosus
Telomerase
Issue Date2010
PublisherSage Publications Ltd. The Journal's web site is located at http://lup.sagepub.com
Citation
Lupus, 2010, v. 19 n. 7, p. 850-859 How to Cite?
AbstractSystemic lupus erythematosus (SLE) has been considered as stem cell disorder. The objective of this study was to examine the phenotype, growth and immunomodulatory effect of mesenchymal stem cells (MSCs) from SLE patients compared with those from age- and sex-matched healthy donors. MSCs were expanded from bone marrow aspirate and were examined for morphological appearance, quantified in different passages to determine growth rate and evaluated for ability of adipogenesis and osteogenesis. Telomerase activity was measured by telomerase repeat amplification protocol. The immunomodulatory effect of MSCs was evaluated by mixed lymphocyte reaction. MSCs from SLE patients were found to be bigger and flattened in appearance after passage 3 and demonstrated slower growth rate compared with fibroblast-like MSCs from normal controls. These cells were not able to reach confluence after passage 4. Telomerase activity was upregulated in five SLE patients mostly with active disease compared with two with negative expression with lesser activity. MSCs from SLE patients were, otherwise, comparable to normal controls in terms of their surface marker (CD73, CD90 and CD105) expression and extent of suppression on proliferation of allogeneic T lymphocytes. In conclusion, MSCs from SLE demonstrated early signs of senescence which may be a corollary of active lupus or a contributory factor to disease pathogenesis. © 2010 The Author(s).
Persistent Identifierhttp://hdl.handle.net/10722/125070
ISSN
2015 Impact Factor: 2.118
2015 SCImago Journal Rankings: 0.878
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong
Funding Information:

This study was supported by research funding from the University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorNie, Yen_HK
dc.contributor.authorLau, CSen_HK
dc.contributor.authorLie, AKWen_HK
dc.contributor.authorChan, GCFen_HK
dc.contributor.authorMok, MYen_HK
dc.date.accessioned2010-10-31T11:09:43Z-
dc.date.available2010-10-31T11:09:43Z-
dc.date.issued2010en_HK
dc.identifier.citationLupus, 2010, v. 19 n. 7, p. 850-859en_HK
dc.identifier.issn0961-2033en_HK
dc.identifier.urihttp://hdl.handle.net/10722/125070-
dc.description.abstractSystemic lupus erythematosus (SLE) has been considered as stem cell disorder. The objective of this study was to examine the phenotype, growth and immunomodulatory effect of mesenchymal stem cells (MSCs) from SLE patients compared with those from age- and sex-matched healthy donors. MSCs were expanded from bone marrow aspirate and were examined for morphological appearance, quantified in different passages to determine growth rate and evaluated for ability of adipogenesis and osteogenesis. Telomerase activity was measured by telomerase repeat amplification protocol. The immunomodulatory effect of MSCs was evaluated by mixed lymphocyte reaction. MSCs from SLE patients were found to be bigger and flattened in appearance after passage 3 and demonstrated slower growth rate compared with fibroblast-like MSCs from normal controls. These cells were not able to reach confluence after passage 4. Telomerase activity was upregulated in five SLE patients mostly with active disease compared with two with negative expression with lesser activity. MSCs from SLE patients were, otherwise, comparable to normal controls in terms of their surface marker (CD73, CD90 and CD105) expression and extent of suppression on proliferation of allogeneic T lymphocytes. In conclusion, MSCs from SLE demonstrated early signs of senescence which may be a corollary of active lupus or a contributory factor to disease pathogenesis. © 2010 The Author(s).en_HK
dc.languageengen_HK
dc.publisherSage Publications Ltd. The Journal's web site is located at http://lup.sagepub.comen_HK
dc.relation.ispartofLupusen_HK
dc.rightsLupus. Copyright © Sage Publications Ltd.-
dc.subjectAutoimmune diseaseen_HK
dc.subjectMesenchymal stem cellsen_HK
dc.subjectSystemic lupus erythematosusen_HK
dc.subjectTelomeraseen_HK
dc.titleDefective phenotype of mesenchymal stem cells in patients with systemic lupus erythematosusen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0961-2033&volume=19&issue=7&spage=850&epage=859&date=2010&atitle=Defective+phenotype+of+mesenchymal+stem+cells+in+patients+with+systemic+lupus+erythematosusen_HK
dc.identifier.emailLau, CS:cslau@hku.hken_HK
dc.identifier.emailChan, GCF:gcfchan@hkucc.hku.hken_HK
dc.identifier.emailMok, MY:temy@hkucc.hku.hken_HK
dc.identifier.authorityLau, CS=rp01348en_HK
dc.identifier.authorityChan, GCF=rp00431en_HK
dc.identifier.authorityMok, MY=rp00490en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1177/0961203309361482en_HK
dc.identifier.pmid20511276-
dc.identifier.scopuseid_2-s2.0-77952921223en_HK
dc.identifier.hkuros174110en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77952921223&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue7en_HK
dc.identifier.spage850en_HK
dc.identifier.epage859en_HK
dc.identifier.isiWOS:000278117200009-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridNie, Y=15072918200en_HK
dc.identifier.scopusauthoridLau, CS=14035682100en_HK
dc.identifier.scopusauthoridLie, AKW=7004510870en_HK
dc.identifier.scopusauthoridChan, GCF=16160154400en_HK
dc.identifier.scopusauthoridMok, MY=7006024184en_HK

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