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Article: Dynamic MicroRNA expression programs during cardiac differentiation of human embryonic stem cells: Role for miR-499

TitleDynamic MicroRNA expression programs during cardiac differentiation of human embryonic stem cells: Role for miR-499
Authors
KeywordsCardiomyocyte
Differentiation
Heart
Human embryonic stem cells
Microarrays
MicroRNA
Issue Date2010
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://circgenetics.ahajournals.org/
Citation
Circulation: Cardiovascular Genetics, 2010, v. 3 n. 5, p. 426-435 How to Cite?
AbstractBackground-MicroRNAs (miRNAs) are a newly discovered endogenous class of small, noncoding RNAs that play important posttranscriptional regulatory roles by targeting messenger RNAs for cleavage or translational repression. Human embryonic stem cells are known to express miRNAs that are often undetectable in adult organs, and a growing body of evidence has implicated miRNAs as important arbiters of heart development and disease. Methods and Results-To better understand the transition between the human embryonic and cardiac "miRNA-omes," we report here the first miRNA profiling study of cardiomyocytes derived from human embryonic stem cells. Analyzing 711 unique miRNAs, we have identified several interesting miRNAs, including miR-1, -133, and -208, that have been previously reported to be involved in cardiac development and disease and that show surprising patterns of expression across our samples. We also identified novel miRNAs, such as miR-499, that are strongly associated with cardiac differentiation and that share many predicted targets with miR-208. Overexpression of miR-499 and -1 resulted in upregulation of important cardiac myosin heavy-chain genes in embryoid bodies; miR-499 overexpression also caused upregulation of the cardiac transcription factor MEF2C. Conclusions-Taken together, our data give significant insight into the regulatory networks that govern human embryonic stem cell differentiation and highlight the ability of miRNAs to perturb, and even control, the genes that are involved in cardiac specification of human embryonic stem cells. © 2010 American Heart Association, Inc.
DescriptionComment in Circ Cardiovasc Genet. 2011 Feb 1;4(1):e3; author reply e4.
Persistent Identifierhttp://hdl.handle.net/10722/125039
ISSN
2014 Impact Factor: 4.600
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Stanford Bio-X Fellowship
National Institutes of HealthDP2 OD004437
HL099776
HL089027
CIRMRC1-00151-1
BWF
Funding Information:

This work was supported by a Stanford Bio-X Fellowship (to K.D.W.), CIRM RC1-00151-1 (O.J.A. and J.J.A.B.), and National Institutes of Health grants DP2 OD004437, HL099776, HL089027, and BWF (to J.C.W.).

References

 

DC FieldValueLanguage
dc.contributor.authorWilson, KDen_HK
dc.contributor.authorHu, Sen_HK
dc.contributor.authorVenkatasubrahmanyam, Sen_HK
dc.contributor.authorFu, JDen_HK
dc.contributor.authorSun, Nen_HK
dc.contributor.authorAbilez, OJen_HK
dc.contributor.authorBaugh, JJAen_HK
dc.contributor.authorJia, Fen_HK
dc.contributor.authorGhosh, Zen_HK
dc.contributor.authorLi, RAen_HK
dc.contributor.authorButte, AJen_HK
dc.contributor.authorWu, JCen_HK
dc.date.accessioned2010-10-31T11:08:02Z-
dc.date.available2010-10-31T11:08:02Z-
dc.date.issued2010en_HK
dc.identifier.citationCirculation: Cardiovascular Genetics, 2010, v. 3 n. 5, p. 426-435en_HK
dc.identifier.issn1942-325Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/125039-
dc.descriptionComment in Circ Cardiovasc Genet. 2011 Feb 1;4(1):e3; author reply e4.-
dc.description.abstractBackground-MicroRNAs (miRNAs) are a newly discovered endogenous class of small, noncoding RNAs that play important posttranscriptional regulatory roles by targeting messenger RNAs for cleavage or translational repression. Human embryonic stem cells are known to express miRNAs that are often undetectable in adult organs, and a growing body of evidence has implicated miRNAs as important arbiters of heart development and disease. Methods and Results-To better understand the transition between the human embryonic and cardiac "miRNA-omes," we report here the first miRNA profiling study of cardiomyocytes derived from human embryonic stem cells. Analyzing 711 unique miRNAs, we have identified several interesting miRNAs, including miR-1, -133, and -208, that have been previously reported to be involved in cardiac development and disease and that show surprising patterns of expression across our samples. We also identified novel miRNAs, such as miR-499, that are strongly associated with cardiac differentiation and that share many predicted targets with miR-208. Overexpression of miR-499 and -1 resulted in upregulation of important cardiac myosin heavy-chain genes in embryoid bodies; miR-499 overexpression also caused upregulation of the cardiac transcription factor MEF2C. Conclusions-Taken together, our data give significant insight into the regulatory networks that govern human embryonic stem cell differentiation and highlight the ability of miRNAs to perturb, and even control, the genes that are involved in cardiac specification of human embryonic stem cells. © 2010 American Heart Association, Inc.en_HK
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://circgenetics.ahajournals.org/en_HK
dc.relation.ispartofCirculation: Cardiovascular Geneticsen_HK
dc.subjectCardiomyocyteen_HK
dc.subjectDifferentiationen_HK
dc.subjectHearten_HK
dc.subjectHuman embryonic stem cellsen_HK
dc.subjectMicroarraysen_HK
dc.subjectMicroRNAen_HK
dc.subject.meshCell Differentiation - physiology-
dc.subject.meshEmbryonic Stem Cells - cytology - physiology-
dc.subject.meshGene Expression Regulation-
dc.subject.meshHeart - embryology - growth and development-
dc.subject.meshMicroRNAs - genetics - metabolism-
dc.titleDynamic MicroRNA expression programs during cardiac differentiation of human embryonic stem cells: Role for miR-499en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1942-325X&volume=3&issue=5&spage=426&epage=435&date=2010&atitle=Dynamic+microRNA+expression+programs+during+cardiac+differentiation+of+human+embryonic+stem+cells:+role+for+miR-499-
dc.identifier.emailLi, RA:ronaldli@hkucc.hku.hken_HK
dc.identifier.authorityLi, RA=rp01352en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1161/CIRCGENETICS.109.934281en_HK
dc.identifier.pmid20733065-
dc.identifier.pmcidPMC3057038-
dc.identifier.scopuseid_2-s2.0-78649366701en_HK
dc.identifier.hkuros182829en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78649366701&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume3en_HK
dc.identifier.issue5en_HK
dc.identifier.spage426en_HK
dc.identifier.epage435en_HK
dc.identifier.isiWOS:000283163100006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWilson, KD=24726465500en_HK
dc.identifier.scopusauthoridHu, S=35292549800en_HK
dc.identifier.scopusauthoridVenkatasubrahmanyam, S=6506471145en_HK
dc.identifier.scopusauthoridFu, JD=7401722481en_HK
dc.identifier.scopusauthoridSun, N=26639877100en_HK
dc.identifier.scopusauthoridAbilez, OJ=8834134300en_HK
dc.identifier.scopusauthoridBaugh, JJA=37030576100en_HK
dc.identifier.scopusauthoridJia, F=8644628500en_HK
dc.identifier.scopusauthoridGhosh, Z=9634383900en_HK
dc.identifier.scopusauthoridLi, RA=7404724466en_HK
dc.identifier.scopusauthoridButte, AJ=7003333396en_HK
dc.identifier.scopusauthoridWu, JC=7409253259en_HK

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