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Article: Role of a novel zebrafish nup98 during embryonic development

TitleRole of a novel zebrafish nup98 during embryonic development
Authors
Issue Date2010
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/exphem
Citation
Experimental Hematology, 2010, v. 38 n. 11, p. 1014-1021.e2 How to Cite?
AbstractObjective: The nucleoporin NUP98 is a component of the nuclear pore complex that regulates nucleocytoplasmic trafficking. It has been characterized in acute myeloid leukemia as a fusion partner during chromosomal translocation. In this study, we identified a zebrafish nup98 gene and examined its role in embryonic development. Materials and Methods: Two expressed sequence tags with translated sequences homologous to human NUP98 were identified. The gene was cloned by polymerase chain reaction from complementary DNA of zebrafish embryos. Cellular functions of zebrafish NUP98 were investigated in HeLa cells. nup98 expression and developmental functions in zebrafish embryos were investigated by whole-mount in situ hybridization and morpholino knockdown. Results: Protein sequence of zebrafish nup98 shared 65% identity with its human homolog. Ectopic expression of zebrafish nup98 rescued the defective messenger RNA export due to human NUP98 knockdown in HeLa cells. In zebrafish embryos, nup98 was expressed diffusely in eyes and the developing brain since 18 hours postfertilization. Knockdown of nup98 with morpholino upregulated pu.1 expression by 39% ± 15% (p = 0.0153) and scl expression by 36% ± 7.6% (p = 0.0017). Expression of genes associated with erythropoiesis was unchanged. The morphants also developed intracranial hemorrhage at 48 hours postfertilization due to defective blood vessel development. Conclusions: A novel zebrafish nup98 was identified and it serves a role in nucleocytoplasmic trafficking similar to human NUP98. During development, it modulates hematopoietic stem cell and early myeloid development and maintains the integrity of cranial vasculature in the developing central nervous system. © 2010 ISEH - Society for Hematology and Stem Cells.
Persistent Identifierhttp://hdl.handle.net/10722/125028
ISSN
2014 Impact Factor: 2.475
2013 SCImago Journal Rankings: 1.462
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong KongHKU 769809M
HKU 7520/06M
Strategy Theme of Cancer Stem Cell LSK200807176190
Funding Information:

We thank the Confocal Microscopy Core Facility LKS Faculty of Medicine HKU and acknowledge the pcDNA3 mRFP plasmid from Dr Doug Golenbock from Addgene This work was supported by General Research Fund (HKU 769809M HKU 7520/06M) a small project fund from the University of Hong Kong (200807176190) and a fund from the Strategy Theme of Cancer Stem Cell LSK Faculty of Medicine HKU

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DC FieldValueLanguage
dc.contributor.authorFung, TKen_HK
dc.contributor.authorChung, MISen_HK
dc.contributor.authorLiang, Ren_HK
dc.contributor.authorLeung, AYHen_HK
dc.date.accessioned2010-10-31T11:07:26Z-
dc.date.available2010-10-31T11:07:26Z-
dc.date.issued2010en_HK
dc.identifier.citationExperimental Hematology, 2010, v. 38 n. 11, p. 1014-1021.e2en_HK
dc.identifier.issn0301-472Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/125028-
dc.description.abstractObjective: The nucleoporin NUP98 is a component of the nuclear pore complex that regulates nucleocytoplasmic trafficking. It has been characterized in acute myeloid leukemia as a fusion partner during chromosomal translocation. In this study, we identified a zebrafish nup98 gene and examined its role in embryonic development. Materials and Methods: Two expressed sequence tags with translated sequences homologous to human NUP98 were identified. The gene was cloned by polymerase chain reaction from complementary DNA of zebrafish embryos. Cellular functions of zebrafish NUP98 were investigated in HeLa cells. nup98 expression and developmental functions in zebrafish embryos were investigated by whole-mount in situ hybridization and morpholino knockdown. Results: Protein sequence of zebrafish nup98 shared 65% identity with its human homolog. Ectopic expression of zebrafish nup98 rescued the defective messenger RNA export due to human NUP98 knockdown in HeLa cells. In zebrafish embryos, nup98 was expressed diffusely in eyes and the developing brain since 18 hours postfertilization. Knockdown of nup98 with morpholino upregulated pu.1 expression by 39% ± 15% (p = 0.0153) and scl expression by 36% ± 7.6% (p = 0.0017). Expression of genes associated with erythropoiesis was unchanged. The morphants also developed intracranial hemorrhage at 48 hours postfertilization due to defective blood vessel development. Conclusions: A novel zebrafish nup98 was identified and it serves a role in nucleocytoplasmic trafficking similar to human NUP98. During development, it modulates hematopoietic stem cell and early myeloid development and maintains the integrity of cranial vasculature in the developing central nervous system. © 2010 ISEH - Society for Hematology and Stem Cells.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/exphemen_HK
dc.relation.ispartofExperimental Hematologyen_HK
dc.subject.meshAmino Acid Sequence-
dc.subject.meshEmbryo, Nonmammalian - embryology - metabolism-
dc.subject.meshNuclear Pore Complex Proteins - genetics - metabolism - physiology-
dc.subject.meshZebrafish - embryology - genetics-
dc.subject.meshZebrafish Proteins - genetics - metabolism - physiology-
dc.titleRole of a novel zebrafish nup98 during embryonic developmenten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0301-472X&volume=38&issue=11&spage=1014&epage=1021&date=2010&atitle=Role+of+a+novel+zebrafish+nup98+during+embryonic+developmenten_HK
dc.identifier.emailLiang, R:rliang@hku.hken_HK
dc.identifier.emailLeung, AYH:ayhleung@hku.hken_HK
dc.identifier.authorityLiang, R=rp00345en_HK
dc.identifier.authorityLeung, AYH=rp00265en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.exphem.2010.07.010en_HK
dc.identifier.pmid20696206en_HK
dc.identifier.scopuseid_2-s2.0-78649344144en_HK
dc.identifier.hkuros180358en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78649344144&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume38en_HK
dc.identifier.issue11en_HK
dc.identifier.spage1014en_HK
dc.identifier.epage1021.e2en_HK
dc.identifier.isiWOS:000284131500005-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectThe roles of survivin in hematopoiesis, angiogenesis and tumorigenesis in a zebrafish knock-down and transgenic model-
dc.relation.projectIs aldehyde dehydrogenase a negative regulator of primitive hematopoiesis during embryonic development?-
dc.identifier.scopusauthoridFung, TK=7102715924en_HK
dc.identifier.scopusauthoridChung, MIS=25958659100en_HK
dc.identifier.scopusauthoridLiang, R=26643224900en_HK
dc.identifier.scopusauthoridLeung, AYH=7403012668en_HK
dc.identifier.citeulike7714800-

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