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Article: Role of a novel zebrafish nup98 during embryonic development
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TitleRole of a novel zebrafish nup98 during embryonic development
 
AuthorsFung, TK1 1
Chung, MIS1
Liang, R1
Leung, AYH1 1
 
Issue Date2010
 
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/exphem
 
CitationExperimental Hematology, 2010, v. 38 n. 11, p. 1014-1021.e2 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.exphem.2010.07.010
 
AbstractObjective: The nucleoporin NUP98 is a component of the nuclear pore complex that regulates nucleocytoplasmic trafficking. It has been characterized in acute myeloid leukemia as a fusion partner during chromosomal translocation. In this study, we identified a zebrafish nup98 gene and examined its role in embryonic development. Materials and Methods: Two expressed sequence tags with translated sequences homologous to human NUP98 were identified. The gene was cloned by polymerase chain reaction from complementary DNA of zebrafish embryos. Cellular functions of zebrafish NUP98 were investigated in HeLa cells. nup98 expression and developmental functions in zebrafish embryos were investigated by whole-mount in situ hybridization and morpholino knockdown. Results: Protein sequence of zebrafish nup98 shared 65% identity with its human homolog. Ectopic expression of zebrafish nup98 rescued the defective messenger RNA export due to human NUP98 knockdown in HeLa cells. In zebrafish embryos, nup98 was expressed diffusely in eyes and the developing brain since 18 hours postfertilization. Knockdown of nup98 with morpholino upregulated pu.1 expression by 39% ± 15% (p = 0.0153) and scl expression by 36% ± 7.6% (p = 0.0017). Expression of genes associated with erythropoiesis was unchanged. The morphants also developed intracranial hemorrhage at 48 hours postfertilization due to defective blood vessel development. Conclusions: A novel zebrafish nup98 was identified and it serves a role in nucleocytoplasmic trafficking similar to human NUP98. During development, it modulates hematopoietic stem cell and early myeloid development and maintains the integrity of cranial vasculature in the developing central nervous system. © 2010 ISEH - Society for Hematology and Stem Cells.
 
ISSN0301-472X
2012 Impact Factor: 2.907
2012 SCImago Journal Rankings: 1.248
 
DOIhttp://dx.doi.org/10.1016/j.exphem.2010.07.010
 
ISI Accession Number IDWOS:000284131500005
Funding AgencyGrant Number
University of Hong KongHKU 769809M
HKU 7520/06M
Strategy Theme of Cancer Stem Cell LSK200807176190
Funding Information:

We thank the Confocal Microscopy Core Facility LKS Faculty of Medicine HKU and acknowledge the pcDNA3 mRFP plasmid from Dr Doug Golenbock from Addgene This work was supported by General Research Fund (HKU 769809M HKU 7520/06M) a small project fund from the University of Hong Kong (200807176190) and a fund from the Strategy Theme of Cancer Stem Cell LSK Faculty of Medicine HKU

 
ReferencesReferences in Scopus
 
GrantsThe roles of survivin in hematopoiesis, angiogenesis and tumorigenesis in a zebrafish knock-down and transgenic model
Is aldehyde dehydrogenase a negative regulator of primitive hematopoiesis during embryonic development?
 
DC FieldValue
dc.contributor.authorFung, TK
 
dc.contributor.authorChung, MIS
 
dc.contributor.authorLiang, R
 
dc.contributor.authorLeung, AYH
 
dc.date.accessioned2010-10-31T11:07:26Z
 
dc.date.available2010-10-31T11:07:26Z
 
dc.date.issued2010
 
dc.description.abstractObjective: The nucleoporin NUP98 is a component of the nuclear pore complex that regulates nucleocytoplasmic trafficking. It has been characterized in acute myeloid leukemia as a fusion partner during chromosomal translocation. In this study, we identified a zebrafish nup98 gene and examined its role in embryonic development. Materials and Methods: Two expressed sequence tags with translated sequences homologous to human NUP98 were identified. The gene was cloned by polymerase chain reaction from complementary DNA of zebrafish embryos. Cellular functions of zebrafish NUP98 were investigated in HeLa cells. nup98 expression and developmental functions in zebrafish embryos were investigated by whole-mount in situ hybridization and morpholino knockdown. Results: Protein sequence of zebrafish nup98 shared 65% identity with its human homolog. Ectopic expression of zebrafish nup98 rescued the defective messenger RNA export due to human NUP98 knockdown in HeLa cells. In zebrafish embryos, nup98 was expressed diffusely in eyes and the developing brain since 18 hours postfertilization. Knockdown of nup98 with morpholino upregulated pu.1 expression by 39% ± 15% (p = 0.0153) and scl expression by 36% ± 7.6% (p = 0.0017). Expression of genes associated with erythropoiesis was unchanged. The morphants also developed intracranial hemorrhage at 48 hours postfertilization due to defective blood vessel development. Conclusions: A novel zebrafish nup98 was identified and it serves a role in nucleocytoplasmic trafficking similar to human NUP98. During development, it modulates hematopoietic stem cell and early myeloid development and maintains the integrity of cranial vasculature in the developing central nervous system. © 2010 ISEH - Society for Hematology and Stem Cells.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationExperimental Hematology, 2010, v. 38 n. 11, p. 1014-1021.e2 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.exphem.2010.07.010
 
dc.identifier.citeulike7714800
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.exphem.2010.07.010
 
dc.identifier.epage1021.e2
 
dc.identifier.hkuros180358
 
dc.identifier.isiWOS:000284131500005
Funding AgencyGrant Number
University of Hong KongHKU 769809M
HKU 7520/06M
Strategy Theme of Cancer Stem Cell LSK200807176190
Funding Information:

We thank the Confocal Microscopy Core Facility LKS Faculty of Medicine HKU and acknowledge the pcDNA3 mRFP plasmid from Dr Doug Golenbock from Addgene This work was supported by General Research Fund (HKU 769809M HKU 7520/06M) a small project fund from the University of Hong Kong (200807176190) and a fund from the Strategy Theme of Cancer Stem Cell LSK Faculty of Medicine HKU

 
dc.identifier.issn0301-472X
2012 Impact Factor: 2.907
2012 SCImago Journal Rankings: 1.248
 
dc.identifier.issue11
 
dc.identifier.openurl
 
dc.identifier.pmid20696206
 
dc.identifier.scopuseid_2-s2.0-78649344144
 
dc.identifier.spage1014
 
dc.identifier.urihttp://hdl.handle.net/10722/125028
 
dc.identifier.volume38
 
dc.languageeng
 
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/exphem
 
dc.publisher.placeUnited States
 
dc.relation.ispartofExperimental Hematology
 
dc.relation.projectThe roles of survivin in hematopoiesis, angiogenesis and tumorigenesis in a zebrafish knock-down and transgenic model
 
dc.relation.projectIs aldehyde dehydrogenase a negative regulator of primitive hematopoiesis during embryonic development?
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAmino Acid Sequence
 
dc.subject.meshEmbryo, Nonmammalian - embryology - metabolism
 
dc.subject.meshNuclear Pore Complex Proteins - genetics - metabolism - physiology
 
dc.subject.meshZebrafish - embryology - genetics
 
dc.subject.meshZebrafish Proteins - genetics - metabolism - physiology
 
dc.titleRole of a novel zebrafish nup98 during embryonic development
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine