Article: Phenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosus

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TitlePhenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosus
AuthorsNie, YJ1
Mok, MY1
Chan, GCF1
Chan, AW1
Jin, O1
Kavikondala, S1
Lie, AKW1
Lau, CS1
Issue Date2010
PublisherBioMed Central Ltd. The Journal's web site is located at http://arthritis-research.com/
CitationArthritis Research And Therapy, 2010, v. 12 n. 3 [How to Cite?]
DOI: http://dx.doi.org/10.1186/ar3018
AbstractIntroduction: Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by autoreactive T and B cells, which are believed to be secondary to deficient dendritic cells (DCs). However, whether DC abnormalities occur during their development in the bone marrow (BM) or in the periphery is not known.Methods: Thirteen patients with SLE and 16 normal controls were recruited. We studied the morphology, phenotype, and functional abilities of bone marrow-derived dendritic cells (BMDCs) generated by using two culture methods: FMS-like tyrosine kinase 3 (Flt3)-ligand (FL) and granulocyte-macrophage colony-stimulating factor (GM-CSF) plus interleukin-4 (IL-4), respectively.Results: BMDCs induced by FL exhibited both myeloid (mDC) and plasmacytoid DC (pDC) features, whereas GM-CSF/IL-4 induced mDC generation. Substantial phenotypic and functional defects of BMDCs were found from patients with SLE at different stages of cell maturation. When compared with healthy controls, SLE immature BM FLDCs expressed higher levels of CCR7. Both immature and mature SLE BM FLDCs expressed higher levels of CD40 and CD86 and induced stronger T-cell proliferation. SLE BM mDCs expressed higher levels of CD40 and CD86 but lower levels of HLA-DR and a lower ability to stimulate T-cell proliferation when compared with control BM mDCs.Conclusions: Our data are in accordance with previous reports that suggest that DCs have a potential pathogenic role in SLE. Defects of these cells are evident during their development in BM. BM mDCs are deficient, whereas BM pDCs, which are part of BM FLDCs, are the likely culprit in inducing autoimmunity in SLE. © 2010 Nie et al.; licensee BioMed Central Ltd.
ISSN1478-6354
2011 Impact Factor: 4.445
2011 SCImago Journal Rankings: 0.482
DOIhttp://dx.doi.org/10.1186/ar3018
ISI Accession Number IDWOS:000280227900036
PubMed Central IDPMC2911875
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorNie, YJ
dc.contributor.authorMok, MY
dc.contributor.authorChan, GCF
dc.contributor.authorChan, AW
dc.contributor.authorJin, O
dc.contributor.authorKavikondala, S
dc.contributor.authorLie, AKW
dc.contributor.authorLau, CS
dc.date.accessioned2010-10-31T11:06:11Z
dc.date.available2010-10-31T11:06:11Z
dc.date.issued2010
dc.description.abstractIntroduction: Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by autoreactive T and B cells, which are believed to be secondary to deficient dendritic cells (DCs). However, whether DC abnormalities occur during their development in the bone marrow (BM) or in the periphery is not known.Methods: Thirteen patients with SLE and 16 normal controls were recruited. We studied the morphology, phenotype, and functional abilities of bone marrow-derived dendritic cells (BMDCs) generated by using two culture methods: FMS-like tyrosine kinase 3 (Flt3)-ligand (FL) and granulocyte-macrophage colony-stimulating factor (GM-CSF) plus interleukin-4 (IL-4), respectively.Results: BMDCs induced by FL exhibited both myeloid (mDC) and plasmacytoid DC (pDC) features, whereas GM-CSF/IL-4 induced mDC generation. Substantial phenotypic and functional defects of BMDCs were found from patients with SLE at different stages of cell maturation. When compared with healthy controls, SLE immature BM FLDCs expressed higher levels of CCR7. Both immature and mature SLE BM FLDCs expressed higher levels of CD40 and CD86 and induced stronger T-cell proliferation. SLE BM mDCs expressed higher levels of CD40 and CD86 but lower levels of HLA-DR and a lower ability to stimulate T-cell proliferation when compared with control BM mDCs.Conclusions: Our data are in accordance with previous reports that suggest that DCs have a potential pathogenic role in SLE. Defects of these cells are evident during their development in BM. BM mDCs are deficient, whereas BM pDCs, which are part of BM FLDCs, are the likely culprit in inducing autoimmunity in SLE. © 2010 Nie et al.; licensee BioMed Central Ltd.
dc.description.naturepublished_or_final_version
dc.identifier.citationArthritis Research And Therapy, 2010, v. 12 n. 3 [How to Cite?]
DOI: http://dx.doi.org/10.1186/ar3018
dc.identifier.doihttp://dx.doi.org/10.1186/ar3018
dc.identifier.hkuros174737
dc.identifier.hkuros195367
dc.identifier.hkuros206497
dc.identifier.isiWOS:000280227900036
dc.identifier.issn1478-6354
2011 Impact Factor: 4.445
2011 SCImago Journal Rankings: 0.482
dc.identifier.issue3
dc.identifier.openurl
dc.identifier.pmcidPMC2911875
dc.identifier.pmid20478074
dc.identifier.scopuseid_2-s2.0-77952255629
dc.identifier.spageR91
dc.identifier.urihttp://hdl.handle.net/10722/125005
dc.identifier.volume12
dc.languageeng
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://arthritis-research.com/
dc.publisher.placeUnited Kingdom
dc.relation.ispartofArthritis Research and Therapy
dc.relation.referencesReferences in Scopus
dc.rightsArthritis Research & Therapy. Copyright © BioMed Central Ltd.
dc.subject.meshAdult
dc.subject.meshBone Marrow Cells - pathology
dc.subject.meshDendritic Cells - drug effects - pathology - physiology
dc.subject.meshLupus Erythematosus, Systemic - pathology - physiopathology
dc.subject.meshPhenotype
dc.titlePhenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosus
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine