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Article: Ectopic expression of systemic RNA interference defective protein in embryonic stem cells

TitleEctopic expression of systemic RNA interference defective protein in embryonic stem cells
Authors
KeywordsEctopic expression
Embryonic stem cells
Gene transfer
RNA interference
SID-1
Issue Date2007
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2007, v. 357 n. 2, p. 480-486 How to Cite?
AbstractRNA interference (RNAi), a post-transcriptional gene silencing mechanism originally described in Caenorhabditis elegans, involves sequence-specific mRNA degradation mediated by double-stranded RNAs (dsRNAs). Passive dsRNA uptake has been uniquely observed in C. elegans due to the expression of systemic RNA interference defective-1 (SID-1). Here we investigated the ability of ectopic SID-1 expression to enable passive cellular uptake of short interfering RNA (siRNA) or double stranded RNA (dsRNA) in pluripotent mouse embryonic stem cells (mESCs). When SID-1-GFP and the Firefly luciferase reporter gene (lucFir) were co-expressed in mESCs, lucFir activity could be suppressed by simple incubation with dsRNAs/siRNAs that were designed to specifically target lucFir. By contrast, suppression was not observed in mESCs expressing lucFir and GFP alone or when either GFP- or SID-1-GFP-expressing cells were incubated with control dsRNAs/siRNAs (non-silencing or Renilla luciferase-specific). These results may lead to high-throughput experimental strategies for studying ESC differentiation and novel approaches to genetically inhibit or eliminate the tumorigenicity of ESCs. © 2007 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/124951
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTsang, SYen_HK
dc.contributor.authorMoore, JCen_HK
dc.contributor.authorHuizen, RVen_HK
dc.contributor.authorChan, CWYen_HK
dc.contributor.authorLi, RAen_HK
dc.date.accessioned2010-10-31T11:03:14Z-
dc.date.available2010-10-31T11:03:14Z-
dc.date.issued2007en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2007, v. 357 n. 2, p. 480-486en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/124951-
dc.description.abstractRNA interference (RNAi), a post-transcriptional gene silencing mechanism originally described in Caenorhabditis elegans, involves sequence-specific mRNA degradation mediated by double-stranded RNAs (dsRNAs). Passive dsRNA uptake has been uniquely observed in C. elegans due to the expression of systemic RNA interference defective-1 (SID-1). Here we investigated the ability of ectopic SID-1 expression to enable passive cellular uptake of short interfering RNA (siRNA) or double stranded RNA (dsRNA) in pluripotent mouse embryonic stem cells (mESCs). When SID-1-GFP and the Firefly luciferase reporter gene (lucFir) were co-expressed in mESCs, lucFir activity could be suppressed by simple incubation with dsRNAs/siRNAs that were designed to specifically target lucFir. By contrast, suppression was not observed in mESCs expressing lucFir and GFP alone or when either GFP- or SID-1-GFP-expressing cells were incubated with control dsRNAs/siRNAs (non-silencing or Renilla luciferase-specific). These results may lead to high-throughput experimental strategies for studying ESC differentiation and novel approaches to genetically inhibit or eliminate the tumorigenicity of ESCs. © 2007 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectEctopic expressionen_HK
dc.subjectEmbryonic stem cellsen_HK
dc.subjectGene transferen_HK
dc.subjectRNA interferenceen_HK
dc.subjectSID-1en_HK
dc.subject.meshCaenorhabditis elegans Proteins - genetics - metabolism-
dc.subject.meshEmbryonic Stem Cells - metabolism-
dc.subject.meshGene Silencing - physiology-
dc.subject.meshKidney - embryology - metabolism-
dc.subject.meshMembrane Proteins - genetics - metabolism-
dc.titleEctopic expression of systemic RNA interference defective protein in embryonic stem cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=357&issue=2&spage=480&epage=486&date=2007&atitle=Ectopic+expression+of+systemic+RNA+interference+defective+protein+in+embryonic+stem+cells-
dc.identifier.emailChan, CWY:camchan@hku.hken_HK
dc.identifier.emailLi, RA:ronaldli@hkucc.hku.hken_HK
dc.identifier.authorityChan, CWY=rp01311en_HK
dc.identifier.authorityLi, RA=rp01352en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.bbrc.2007.03.187en_HK
dc.identifier.pmid17434453-
dc.identifier.pmcidPMC2464293-
dc.identifier.scopuseid_2-s2.0-34247141577en_HK
dc.identifier.hkuros183050en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34247141577&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume357en_HK
dc.identifier.issue2en_HK
dc.identifier.spage480en_HK
dc.identifier.epage486en_HK
dc.identifier.isiWOS:000246253700025-
dc.publisher.placeUnited Statesen_HK
dc.identifier.f10001097364-
dc.identifier.scopusauthoridTsang, SY=7102255908en_HK
dc.identifier.scopusauthoridMoore, JC=35185459800en_HK
dc.identifier.scopusauthoridHuizen, RV=16230204300en_HK
dc.identifier.scopusauthoridChan, CWY=12240386600en_HK
dc.identifier.scopusauthoridLi, RA=7404724466en_HK

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