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Article: Role of mitogen-activated protein kinase in Zn-BC-AM PDT-induced apoptosis in nasopharyngeal carcinoma cells

TitleRole of mitogen-activated protein kinase in Zn-BC-AM PDT-induced apoptosis in nasopharyngeal carcinoma cells
Authors
KeywordsApoptosis
Isoforms
MAPK
P38
PD169316
Photodynamic therapy
Issue Date2010
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0263-6484/
Citation
Cell Biochemistry And Function, 2010, v. 28 n. 3, p. 239-248 How to Cite?
AbstractPhotodynamic therapy (PDT) with a recently developed photosensitizer Zn-BC-AM was found to effectively induce apoptosis in a welldifferentiated nasopharyngeal carcinoma (NPC) HK-1 cell line. Sustained activation of p38 mitogen-activated protein kinase (MAPK) and cjun N-terminal kinase (JNK) as well as a transient increase in activation of extracellular signal-regulated kinase (ERK) were observed immediately after Zn-BC-AM PDT. A commonly used p38 MAPK/JNK pharmacological inhibitor PD169316 was found to reduce PDTinduced apoptosis of HK-1 cells. PD169316 also prevented the loss of Bcl-2 and Bcl-xL in PDT-treated HK-1 cells. However, inhibition of JNK with SP600125 had no effect on Zn-BC-AM PDT-induced apoptosis while inhibition of ERK with PD98059 or p38 MAPK with SB203580 significantly increased Zn-BC-AM PDT-induced apoptosis. Further study showed that knockdown of the p38b isoform with siRNA also increased Zn-BC-AM PDT-induced apoptosis, indicating that the anti-apoptotic effect of PD169316 in PDT-treated HK-1 cells was probably independent of p38 MAPK or JNK activation. Taken together, the results suggest that inhibition of p38b and ERK may enhance the therapeutic efficacy of Zn-BC-AM PDTon NPC cells. It should be noted that data only based on the use of PD169316 should be interpreted in caution. Copyright © 2010 John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/124617
ISSN
2015 Impact Factor: 2.016
2015 SCImago Journal Rankings: 0.887
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council of Hong KongHKBU 2458/06M
Faculty DevelopmentFRG/07-0811-57
Funding Information:

This work was supported by the Research Grants Council of Hong Kong (project no. HKBU 2458/06M) and the Faculty Development Grant (no. FRG/07-0811-57).

References

 

DC FieldValueLanguage
dc.contributor.authorKoon, HKen_HK
dc.contributor.authorChan, PSen_HK
dc.contributor.authorWu, ZGen_HK
dc.contributor.authorWong, RNSen_HK
dc.contributor.authorLung, MLen_HK
dc.contributor.authorChang, CKen_HK
dc.contributor.authorMak, NKen_HK
dc.date.accessioned2010-10-31T10:44:29Z-
dc.date.available2010-10-31T10:44:29Z-
dc.date.issued2010en_HK
dc.identifier.citationCell Biochemistry And Function, 2010, v. 28 n. 3, p. 239-248en_HK
dc.identifier.issn0263-6484en_HK
dc.identifier.urihttp://hdl.handle.net/10722/124617-
dc.description.abstractPhotodynamic therapy (PDT) with a recently developed photosensitizer Zn-BC-AM was found to effectively induce apoptosis in a welldifferentiated nasopharyngeal carcinoma (NPC) HK-1 cell line. Sustained activation of p38 mitogen-activated protein kinase (MAPK) and cjun N-terminal kinase (JNK) as well as a transient increase in activation of extracellular signal-regulated kinase (ERK) were observed immediately after Zn-BC-AM PDT. A commonly used p38 MAPK/JNK pharmacological inhibitor PD169316 was found to reduce PDTinduced apoptosis of HK-1 cells. PD169316 also prevented the loss of Bcl-2 and Bcl-xL in PDT-treated HK-1 cells. However, inhibition of JNK with SP600125 had no effect on Zn-BC-AM PDT-induced apoptosis while inhibition of ERK with PD98059 or p38 MAPK with SB203580 significantly increased Zn-BC-AM PDT-induced apoptosis. Further study showed that knockdown of the p38b isoform with siRNA also increased Zn-BC-AM PDT-induced apoptosis, indicating that the anti-apoptotic effect of PD169316 in PDT-treated HK-1 cells was probably independent of p38 MAPK or JNK activation. Taken together, the results suggest that inhibition of p38b and ERK may enhance the therapeutic efficacy of Zn-BC-AM PDTon NPC cells. It should be noted that data only based on the use of PD169316 should be interpreted in caution. Copyright © 2010 John Wiley & Sons, Ltd.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0263-6484/en_HK
dc.relation.ispartofCell Biochemistry and Functionen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThe definitive version is available at www3.interscience.wiley.com-
dc.subjectApoptosisen_HK
dc.subjectIsoformsen_HK
dc.subjectMAPKen_HK
dc.subjectP38en_HK
dc.subjectPD169316en_HK
dc.subjectPhotodynamic therapyen_HK
dc.titleRole of mitogen-activated protein kinase in Zn-BC-AM PDT-induced apoptosis in nasopharyngeal carcinoma cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0263-6484&volume=28&issue=3&spage=239&epage=248&date=2010&atitle=Role+of+mitogen-activated+protein+kinase+in+Zn-BC-AM+PDT-induced+apoptosis+in+nasopharyngeal+carcinoma+cells-
dc.identifier.emailLung, ML:mlilung@hku.hken_HK
dc.identifier.authorityLung, ML=rp00300en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1002/cbf.1650en_HK
dc.identifier.pmid20373469en_HK
dc.identifier.scopuseid_2-s2.0-77953558733en_HK
dc.identifier.hkuros174225en_HK
dc.identifier.hkuros186341-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77953558733&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume28en_HK
dc.identifier.issue3en_HK
dc.identifier.spage239en_HK
dc.identifier.epage248en_HK
dc.identifier.isiWOS:000276875200010-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridKoon, HK=12766487800en_HK
dc.identifier.scopusauthoridChan, PS=35075787200en_HK
dc.identifier.scopusauthoridWu, ZG=14071894400en_HK
dc.identifier.scopusauthoridWong, RNS=7402126957en_HK
dc.identifier.scopusauthoridLung, ML=7006411788en_HK
dc.identifier.scopusauthoridChang, CK=7407039448en_HK
dc.identifier.scopusauthoridMak, NK=35582657000en_HK

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