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Article: Characterization of Fe-hydrogenase genes diversity and hydrogen-producing population in an acidophilic sludge

TitleCharacterization of Fe-hydrogenase genes diversity and hydrogen-producing population in an acidophilic sludge
Authors
KeywordsAcidophilic
Fe-hydrogenase gene
Hydrogen production
Primer
Quantitative real-time polymerase chain reaction
Issue Date2006
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jbiotec
Citation
Journal Of Biotechnology, 2006, v. 126 n. 3, p. 357-364 How to Cite?
AbstractAccording to the DNA sequences of six Fe-hydrogenase genes (FHG) of Clostridium species retrieved from the GenBank, a set of primers specific for Fe-hydrogenase genes were identified from their common conserved regions. The length of DNA fragments amplified using these two primers averaged 313 bps. This primer set was then used to investigate the FHG diversity in an acidophilic rice-degrading sludge by methods based on polymerase chain reaction (PCR). Eight new Fe-hydrogenase gene fragments were identified from the sludge, as a result. Similarity based on amino acids among the 14 hydrogenase genes (8 newly found plus 6 known ones) was 39-97%, which is comparable to the similarity of 41-82% among the 6 known hydrogenase genes alone. The low similarity indicates a great diversity on Fe-hydrogenase among the Clostridium species. The primer set was then used to monitor the change of hydrogen-producing microbial population in a batch reaction using the technique of quantitative real-time polymerase chain reaction (qRT-PCR) with SYBR Green I as the fluorescent reagent. Results showed that the hydrogen producers had an average generation time of 4.2 h, and a production rate of 7.0 × 1016 H2-molecule cell-1 h-1. © 2006 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/124611
ISSN
2015 Impact Factor: 2.667
2015 SCImago Journal Rankings: 1.064
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFang, HHPen_HK
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorLi, Cen_HK
dc.date.accessioned2010-10-31T10:44:06Z-
dc.date.available2010-10-31T10:44:06Z-
dc.date.issued2006en_HK
dc.identifier.citationJournal Of Biotechnology, 2006, v. 126 n. 3, p. 357-364en_HK
dc.identifier.issn0168-1656en_HK
dc.identifier.urihttp://hdl.handle.net/10722/124611-
dc.description.abstractAccording to the DNA sequences of six Fe-hydrogenase genes (FHG) of Clostridium species retrieved from the GenBank, a set of primers specific for Fe-hydrogenase genes were identified from their common conserved regions. The length of DNA fragments amplified using these two primers averaged 313 bps. This primer set was then used to investigate the FHG diversity in an acidophilic rice-degrading sludge by methods based on polymerase chain reaction (PCR). Eight new Fe-hydrogenase gene fragments were identified from the sludge, as a result. Similarity based on amino acids among the 14 hydrogenase genes (8 newly found plus 6 known ones) was 39-97%, which is comparable to the similarity of 41-82% among the 6 known hydrogenase genes alone. The low similarity indicates a great diversity on Fe-hydrogenase among the Clostridium species. The primer set was then used to monitor the change of hydrogen-producing microbial population in a batch reaction using the technique of quantitative real-time polymerase chain reaction (qRT-PCR) with SYBR Green I as the fluorescent reagent. Results showed that the hydrogen producers had an average generation time of 4.2 h, and a production rate of 7.0 × 1016 H2-molecule cell-1 h-1. © 2006 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jbiotecen_HK
dc.relation.ispartofJournal of Biotechnologyen_HK
dc.subjectAcidophilicen_HK
dc.subjectFe-hydrogenase geneen_HK
dc.subjectHydrogen productionen_HK
dc.subjectPrimeren_HK
dc.subjectQuantitative real-time polymerase chain reactionen_HK
dc.subject.meshClostridium - classification - enzymology - genetics-
dc.subject.meshHydrogen - metabolism-
dc.subject.meshHydrogenase - chemistry - genetics - metabolism-
dc.subject.meshIron-Sulfur Proteins - chemistry - genetics - metabolism-
dc.subject.meshSewage - microbiology-
dc.titleCharacterization of Fe-hydrogenase genes diversity and hydrogen-producing population in an acidophilic sludgeen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0168-1656&volume=126&issue=3&spage=357&epage=364&date=2006&atitle=Characterization+of+Fe-hydrogenase+genes+diversity+and+hydrogen-producing+population+in+an+acidophilic+sludge-
dc.identifier.emailFang, HHP:hrechef@hkucc.hku.hken_HK
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_HK
dc.identifier.authorityFang, HHP=rp00115en_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jbiotec.2006.04.023en_HK
dc.identifier.pmid16730831-
dc.identifier.scopuseid_2-s2.0-33749515929en_HK
dc.identifier.hkuros138085en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33749515929&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume126en_HK
dc.identifier.issue3en_HK
dc.identifier.spage357en_HK
dc.identifier.epage364en_HK
dc.identifier.isiWOS:000242252300011-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridFang, HHP=7402542625en_HK
dc.identifier.scopusauthoridZhang, T=24470677400en_HK
dc.identifier.scopusauthoridLi, C=12804810200en_HK

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