Conference Paper: Class 1 integronase gene and tetracycline resistance genes tetA and tetC in different water environments of Jiangsu Province, China

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TitleClass 1 integronase gene and tetracycline resistance genes tetA and tetC in different water environments of Jiangsu Province, China
AuthorsZhang, X1 2
Wu, B1
Zhang, Y1
Zhang, T2
Yang, L1
Fang, HHP2
Ford, T3
Cheng, S1
KeywordsAntibiotic resistance gene
Tetracycline
Integron
Water environment
Drinking water
Issue Date2009
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0963-9292
CitationEcotoxicology, 2009, v. 18 n. 6, p. 652-660 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10646-009-0332-3
AbstractClass 1 integronase gene (intI1) and tetracycline resistance genes (tetA and tetC) from various environmental sites in Jiangsu Province (China) were detected using qualitative PCR (polymerase chain reaction) and quantified with SYBR Green-based qRT-PCR (quantitative real-time PCR) in this study. Qualitative PCR assays demonstrated that intI1, tetA and tetC occurred in the water environments of Taihu Lake, the Nanjing section of the Yangtze River, a sewage treatment plant (STP) in Nanjing City, and two drinking water treating bioreactors. qRT-PCR results showed that abundance of intI1 in lake water and sediments was lower than the tet genes, for a given sample site and date (P < 0.05). On a volumetric basis, lake sediments contained higher concentrations of the three genes by four to five orders of magnitude than water samples, and lake water and sediments sampled in April contained fewer copies of all the genes than the samples collected in June and August (P < 0.05). The levels of intI1, tetA and tetC in the Yangtze River water increased significantly after the river flowed through Nanjing City (P < 0.05). 94.1% integron, 97.2% tetA and 98.3% tetC were removed by the activated sludge process in the STP, and more than 80% of each gene was removed in both of the two biofilters in terms of relative concentration based on sample volume. However, on the basis of DNA mass, lower removals were obtained for both the activated sludge and biofiltration processes. © Springer Science+Business Media, LLC 2009.
ISSN0963-9292
2011 Impact Factor: 2.355
2011 SCImago Journal Rankings: 0.141
DOIhttp://dx.doi.org/10.1007/s10646-009-0332-3
ISI Accession Number IDWOS:000267669500003
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorZhang, X
dc.contributor.authorWu, B
dc.contributor.authorZhang, Y
dc.contributor.authorZhang, T
dc.contributor.authorYang, L
dc.contributor.authorFang, HHP
dc.contributor.authorFord, T
dc.contributor.authorCheng, S
dc.date.accessioned2010-10-31T10:42:35Z
dc.date.available2010-10-31T10:42:35Z
dc.date.issued2009
dc.description.abstractClass 1 integronase gene (intI1) and tetracycline resistance genes (tetA and tetC) from various environmental sites in Jiangsu Province (China) were detected using qualitative PCR (polymerase chain reaction) and quantified with SYBR Green-based qRT-PCR (quantitative real-time PCR) in this study. Qualitative PCR assays demonstrated that intI1, tetA and tetC occurred in the water environments of Taihu Lake, the Nanjing section of the Yangtze River, a sewage treatment plant (STP) in Nanjing City, and two drinking water treating bioreactors. qRT-PCR results showed that abundance of intI1 in lake water and sediments was lower than the tet genes, for a given sample site and date (P < 0.05). On a volumetric basis, lake sediments contained higher concentrations of the three genes by four to five orders of magnitude than water samples, and lake water and sediments sampled in April contained fewer copies of all the genes than the samples collected in June and August (P < 0.05). The levels of intI1, tetA and tetC in the Yangtze River water increased significantly after the river flowed through Nanjing City (P < 0.05). 94.1% integron, 97.2% tetA and 98.3% tetC were removed by the activated sludge process in the STP, and more than 80% of each gene was removed in both of the two biofilters in terms of relative concentration based on sample volume. However, on the basis of DNA mass, lower removals were obtained for both the activated sludge and biofiltration processes. © Springer Science+Business Media, LLC 2009.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationEcotoxicology, 2009, v. 18 n. 6, p. 652-660 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10646-009-0332-3
dc.identifier.citeulike4784417
dc.identifier.doihttp://dx.doi.org/10.1007/s10646-009-0332-3
dc.identifier.epage660
dc.identifier.hkuros175520
dc.identifier.isiWOS:000267669500003
dc.identifier.issn0963-9292
2011 Impact Factor: 2.355
2011 SCImago Journal Rankings: 0.141
dc.identifier.issue6
dc.identifier.openurl
dc.identifier.pmid19495963
dc.identifier.scopuseid_2-s2.0-67651097650
dc.identifier.spage652
dc.identifier.urihttp://hdl.handle.net/10722/124583
dc.identifier.volume18
dc.languageeng
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0963-9292
dc.publisher.placeUnited States
dc.relation.ispartofEcotoxicology
dc.relation.referencesReferences in Scopus
dc.rightsThe original publication is available at www.springerlink.com
dc.subject.meshAntiporters - genetics
dc.subject.meshBacterial Proteins - genetics
dc.subject.meshChina
dc.subject.meshDNA, Bacterial - genetics
dc.subject.meshEnvironmental Monitoring
dc.subject.meshFresh Water - analysis - microbiology
dc.subject.meshGenes, Bacterial
dc.subject.meshGeologic Sediments - analysis - microbiology
dc.subject.meshIntegrases - genetics
dc.subject.meshRepressor Proteins - genetics
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
dc.subject.meshSewage - analysis - microbiology
dc.subject.meshTetracycline Resistance - genetics
dc.subject.meshWater Microbiology
dc.subject.meshWater Purification
dc.subjectAntibiotic resistance gene
dc.subjectTetracycline
dc.subjectIntegron
dc.subjectWater environment
dc.subjectDrinking water
dc.titleClass 1 integronase gene and tetracycline resistance genes tetA and tetC in different water environments of Jiangsu Province, China
dc.typeConference_Paper
Author Affiliations
  1. Nanjing University
  2. The University of Hong Kong
  3. University of New England