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Conference Paper: Class 1 integronase gene and tetracycline resistance genes tetA and tetC in different water environments of Jiangsu Province, China
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TitleClass 1 integronase gene and tetracycline resistance genes tetA and tetC in different water environments of Jiangsu Province, China
 
AuthorsZhang, X1 2
Wu, B1
Zhang, Y1
Zhang, T2
Yang, L1
Fang, HHP2
Ford, T3
Cheng, S1
 
KeywordsAntibiotic resistance gene
Tetracycline
Integron
Water environment
Drinking water
 
Issue Date2009
 
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0963-9292
 
CitationEcotoxicology, 2009, v. 18 n. 6, p. 652-660 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10646-009-0332-3
 
AbstractClass 1 integronase gene (intI1) and tetracycline resistance genes (tetA and tetC) from various environmental sites in Jiangsu Province (China) were detected using qualitative PCR (polymerase chain reaction) and quantified with SYBR Green-based qRT-PCR (quantitative real-time PCR) in this study. Qualitative PCR assays demonstrated that intI1, tetA and tetC occurred in the water environments of Taihu Lake, the Nanjing section of the Yangtze River, a sewage treatment plant (STP) in Nanjing City, and two drinking water treating bioreactors. qRT-PCR results showed that abundance of intI1 in lake water and sediments was lower than the tet genes, for a given sample site and date (P < 0.05). On a volumetric basis, lake sediments contained higher concentrations of the three genes by four to five orders of magnitude than water samples, and lake water and sediments sampled in April contained fewer copies of all the genes than the samples collected in June and August (P < 0.05). The levels of intI1, tetA and tetC in the Yangtze River water increased significantly after the river flowed through Nanjing City (P < 0.05). 94.1% integron, 97.2% tetA and 98.3% tetC were removed by the activated sludge process in the STP, and more than 80% of each gene was removed in both of the two biofilters in terms of relative concentration based on sample volume. However, on the basis of DNA mass, lower removals were obtained for both the activated sludge and biofiltration processes. © Springer Science+Business Media, LLC 2009.
 
ISSN0963-9292
2013 Impact Factor: 2.500
 
DOIhttp://dx.doi.org/10.1007/s10646-009-0332-3
 
ISI Accession Number IDWOS:000267669500003
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorZhang, X
 
dc.contributor.authorWu, B
 
dc.contributor.authorZhang, Y
 
dc.contributor.authorZhang, T
 
dc.contributor.authorYang, L
 
dc.contributor.authorFang, HHP
 
dc.contributor.authorFord, T
 
dc.contributor.authorCheng, S
 
dc.date.accessioned2010-10-31T10:42:35Z
 
dc.date.available2010-10-31T10:42:35Z
 
dc.date.issued2009
 
dc.description.abstractClass 1 integronase gene (intI1) and tetracycline resistance genes (tetA and tetC) from various environmental sites in Jiangsu Province (China) were detected using qualitative PCR (polymerase chain reaction) and quantified with SYBR Green-based qRT-PCR (quantitative real-time PCR) in this study. Qualitative PCR assays demonstrated that intI1, tetA and tetC occurred in the water environments of Taihu Lake, the Nanjing section of the Yangtze River, a sewage treatment plant (STP) in Nanjing City, and two drinking water treating bioreactors. qRT-PCR results showed that abundance of intI1 in lake water and sediments was lower than the tet genes, for a given sample site and date (P < 0.05). On a volumetric basis, lake sediments contained higher concentrations of the three genes by four to five orders of magnitude than water samples, and lake water and sediments sampled in April contained fewer copies of all the genes than the samples collected in June and August (P < 0.05). The levels of intI1, tetA and tetC in the Yangtze River water increased significantly after the river flowed through Nanjing City (P < 0.05). 94.1% integron, 97.2% tetA and 98.3% tetC were removed by the activated sludge process in the STP, and more than 80% of each gene was removed in both of the two biofilters in terms of relative concentration based on sample volume. However, on the basis of DNA mass, lower removals were obtained for both the activated sludge and biofiltration processes. © Springer Science+Business Media, LLC 2009.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationEcotoxicology, 2009, v. 18 n. 6, p. 652-660 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10646-009-0332-3
 
dc.identifier.citeulike4784417
 
dc.identifier.doihttp://dx.doi.org/10.1007/s10646-009-0332-3
 
dc.identifier.epage660
 
dc.identifier.hkuros175520
 
dc.identifier.isiWOS:000267669500003
 
dc.identifier.issn0963-9292
2013 Impact Factor: 2.500
 
dc.identifier.issue6
 
dc.identifier.openurl
 
dc.identifier.pmid19495963
 
dc.identifier.scopuseid_2-s2.0-67651097650
 
dc.identifier.spage652
 
dc.identifier.urihttp://hdl.handle.net/10722/124583
 
dc.identifier.volume18
 
dc.languageeng
 
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0963-9292
 
dc.publisher.placeUnited States
 
dc.relation.ispartofEcotoxicology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsThe original publication is available at www.springerlink.com
 
dc.subject.meshAntiporters - genetics
 
dc.subject.meshBacterial Proteins - genetics
 
dc.subject.meshChina
 
dc.subject.meshDNA, Bacterial - genetics
 
dc.subject.meshEnvironmental Monitoring
 
dc.subject.meshFresh Water - analysis - microbiology
 
dc.subject.meshGenes, Bacterial
 
dc.subject.meshGeologic Sediments - analysis - microbiology
 
dc.subject.meshIntegrases - genetics
 
dc.subject.meshRepressor Proteins - genetics
 
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
 
dc.subject.meshSewage - analysis - microbiology
 
dc.subject.meshTetracycline Resistance - genetics
 
dc.subject.meshWater Microbiology
 
dc.subject.meshWater Purification
 
dc.subjectAntibiotic resistance gene
 
dc.subjectTetracycline
 
dc.subjectIntegron
 
dc.subjectWater environment
 
dc.subjectDrinking water
 
dc.titleClass 1 integronase gene and tetracycline resistance genes tetA and tetC in different water environments of Jiangsu Province, China
 
dc.typeConference_Paper
 
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Author Affiliations
  1. Nanjing University
  2. The University of Hong Kong
  3. University of New England