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Article: Effects of bone morphogenetic protein 2 on Id expression and neuroblastoma cell differentiation

TitleEffects of bone morphogenetic protein 2 on Id expression and neuroblastoma cell differentiation
Authors
Issue Date2010
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/DIF
Citation
Differentiation, 2010, v. 79 n. 2, p. 84-92 How to Cite?
AbstractBone morphogenetic proteins (BMPs) are secretory signal molecules that have a variety of regulatory functions during embryonic morphogenesis. BMP2 has been shown to induce differentiation in many cell types, mediated through the activation of its target genes: the inhibitors of differentiation (Id1-3) and key transcription factors. In this study, we investigated the effects of BMP2 on mouse neuroblastoma (Neuro2a) cell differentiation and regulation of the expression of Id1-3 and neural-specific transcription factors. Our results showed that BMP2 stimulation upregulated Id1-3 expression at the early stage of application by involvement of the Smad signaling pathway. BMP2 caused phosphorylation of Smad1/5/8 followed by upregulation of Id1-3. Co-incubation with Noggin, a BMP antagonist, or Smad1 siRNA transfection significantly inhibited phosphorylation of Smad1/5/8 and upregulation of Id protein. Furthermore, our results showed that BMP2-induced differentiation of Neuro2a cells into neurons by downregulating the expression of Id1-3 proteins and upregulating the expression of neural-specific transcriptional factors Dlx2, Brn3a, and NeuroD6. The results suggested that the transient upregulation of Id1-3 expression during the early phase of BMP stimulation may play a role in lineage specification and promote differentiation of neuroblastoma cells towards a neuronal phenotype. Subsequently, a coordinated increase in expression of proneural transcription factors and a decrease in Id1-3 expression may culminate in the transition from proliferation to neurogenesis and the terminal neuronal differentiation of neuroblastoma cells. © 2009 International Society of Differentiation.
Persistent Identifierhttp://hdl.handle.net/10722/124503
ISSN
2015 Impact Factor: 2.461
2015 SCImago Journal Rankings: 1.747
ISI Accession Number ID
Funding AgencyGrant Number
Seed Funding Program for Basic Research200611159203
University of Hong Kong and General Research Fund10208603
Hong Kong Research Grants Council
Funding Information:

This study was supported by the Seed Funding Program for Basic Research (No. 200611159203) from the University of Hong Kong and General Research Fund (No. 10208603) from Hong Kong Research Grants Council.

References

 

DC FieldValueLanguage
dc.contributor.authorDu, Yen_HK
dc.contributor.authorYip, Hen_HK
dc.date.accessioned2010-10-31T10:38:03Z-
dc.date.available2010-10-31T10:38:03Z-
dc.date.issued2010en_HK
dc.identifier.citationDifferentiation, 2010, v. 79 n. 2, p. 84-92en_HK
dc.identifier.issn0301-4681en_HK
dc.identifier.urihttp://hdl.handle.net/10722/124503-
dc.description.abstractBone morphogenetic proteins (BMPs) are secretory signal molecules that have a variety of regulatory functions during embryonic morphogenesis. BMP2 has been shown to induce differentiation in many cell types, mediated through the activation of its target genes: the inhibitors of differentiation (Id1-3) and key transcription factors. In this study, we investigated the effects of BMP2 on mouse neuroblastoma (Neuro2a) cell differentiation and regulation of the expression of Id1-3 and neural-specific transcription factors. Our results showed that BMP2 stimulation upregulated Id1-3 expression at the early stage of application by involvement of the Smad signaling pathway. BMP2 caused phosphorylation of Smad1/5/8 followed by upregulation of Id1-3. Co-incubation with Noggin, a BMP antagonist, or Smad1 siRNA transfection significantly inhibited phosphorylation of Smad1/5/8 and upregulation of Id protein. Furthermore, our results showed that BMP2-induced differentiation of Neuro2a cells into neurons by downregulating the expression of Id1-3 proteins and upregulating the expression of neural-specific transcriptional factors Dlx2, Brn3a, and NeuroD6. The results suggested that the transient upregulation of Id1-3 expression during the early phase of BMP stimulation may play a role in lineage specification and promote differentiation of neuroblastoma cells towards a neuronal phenotype. Subsequently, a coordinated increase in expression of proneural transcription factors and a decrease in Id1-3 expression may culminate in the transition from proliferation to neurogenesis and the terminal neuronal differentiation of neuroblastoma cells. © 2009 International Society of Differentiation.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/DIFen_HK
dc.relation.ispartofDifferentiationen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBone Morphogenetic Protein 2 - metabolismen_HK
dc.subject.meshCell Differentiationen_HK
dc.subject.meshDown-Regulationen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInhibitor of Differentiation Proteins - genetics - metabolismen_HK
dc.subject.meshMiceen_HK
dc.subject.meshNeuroblastoma - metabolismen_HK
dc.subject.meshRNA, Small Interfering - metabolismen_HK
dc.subject.meshSignal Transductionen_HK
dc.subject.meshTranscription Factors - genetics - metabolismen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.subject.meshUp-Regulationen_HK
dc.titleEffects of bone morphogenetic protein 2 on Id expression and neuroblastoma cell differentiationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0301-4681&volume=79&spage=84&epage=92&date=2010&atitle=Effects+Of+Bone+Morphogenetic+Protein+2+On+Id+Expression+And+Neuroblastoma+Cell+Differentiationen_HK
dc.identifier.emailYip, H:hkfyip@hku.hken_HK
dc.identifier.authorityYip, H=rp00285en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.diff.2009.10.003en_HK
dc.identifier.pmid19889495-
dc.identifier.scopuseid_2-s2.0-77549084500en_HK
dc.identifier.hkuros176334en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77549084500&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume79en_HK
dc.identifier.issue2en_HK
dc.identifier.spage84en_HK
dc.identifier.epage92en_HK
dc.identifier.isiWOS:000283715200003-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridDu, Y=36993717500en_HK
dc.identifier.scopusauthoridYip, H=7101980864en_HK

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