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Article: Roles of paroxetine and corticosterone on adult mammalian ciliary body cell proliferation

TitleRoles of paroxetine and corticosterone on adult mammalian ciliary body cell proliferation
Authors
Keywords5-bromo-2-deoxyuridine
Cell proliferation
Ciliary body
Corticosterone
Neurogenesis
Paroxetine
Issue Date2010
PublisherZhonghua Yixuehui. The Journal's web site is located at http://www.cmj.org/
Citation
Chinese Medical Journal, 2010, v. 123 n. 10, p. 1305-1310 How to Cite?
AbstractBackground The neurogenesis in retina of adult mammals is generally abolished, and this renders the retina lack of regenerative capacity. Despite this, there is a small population of nestin-positive cells in the ciliary epithelium which retains neurogenic potential. The present study aimed at investigating the effect of two drugs, corticosterone and paroxetine, on the cell proliferation of the ciliary body. Methods Adult Sprague-Dawley rats were given vehicle, corticosterone, paroxetine, or both corticosterone and paroxetine treatment for 14 days. Cell proliferation in the ciliary body was quantified using 5-bromo-2-deoxyuridine (BrdU) immunohistochemistry. Co-labelling of BrdU and stem cell marker was used to phenotype the BrdU immunoreactive cells. Results Corticosterone treatment suppressed while paroxetine treatment increased the cell proliferation of the ciliary body. Co-labelling with cell markers revealed that the BrdU positive cells also showed nestin expression but not glial fibrillary acidic protein (GFAP). Conclusions The results illustrate that proliferation of retinal progenitor cells situated in ciliary body are subjected to regulation by selective serotonin reuptake inhibitors (SSRI) and corticosteroid, which is similar to our previous findings in neurogenic regions in central nervous system (CNS). Paroxetine treatment could reverse the suppressive effect of corticosterone on ciliary body cell proliferation. This provides information for future investigation of retinal stem cell biology and potential treatment of retinal degenerative diseases.
Persistent Identifierhttp://hdl.handle.net/10722/124502
ISSN
2015 Impact Factor: 0.957
2015 SCImago Journal Rankings: 0.428
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Charitable Eye Foundation
Funding Information:

We thank Richel Wong, Kin Chiu and Alex Lau for assistance in the experimental procedure. This research was supported by donation from the Hong Kong Charitable Eye Foundation and Mrs. Annie Tsao Wen Wei.

References

 

DC FieldValueLanguage
dc.contributor.authorWang, Hen_HK
dc.contributor.authorLau, BWMen_HK
dc.contributor.authorYau, SYen_HK
dc.contributor.authorLi, SYen_HK
dc.contributor.authorLeung, Nen_HK
dc.contributor.authorWang, NLen_HK
dc.contributor.authorTang, SWen_HK
dc.contributor.authorLee, Ten_HK
dc.contributor.authorSo, KFen_HK
dc.date.accessioned2010-10-31T10:38:00Z-
dc.date.available2010-10-31T10:38:00Z-
dc.date.issued2010en_HK
dc.identifier.citationChinese Medical Journal, 2010, v. 123 n. 10, p. 1305-1310en_HK
dc.identifier.issn0366-6999en_HK
dc.identifier.urihttp://hdl.handle.net/10722/124502-
dc.description.abstractBackground The neurogenesis in retina of adult mammals is generally abolished, and this renders the retina lack of regenerative capacity. Despite this, there is a small population of nestin-positive cells in the ciliary epithelium which retains neurogenic potential. The present study aimed at investigating the effect of two drugs, corticosterone and paroxetine, on the cell proliferation of the ciliary body. Methods Adult Sprague-Dawley rats were given vehicle, corticosterone, paroxetine, or both corticosterone and paroxetine treatment for 14 days. Cell proliferation in the ciliary body was quantified using 5-bromo-2-deoxyuridine (BrdU) immunohistochemistry. Co-labelling of BrdU and stem cell marker was used to phenotype the BrdU immunoreactive cells. Results Corticosterone treatment suppressed while paroxetine treatment increased the cell proliferation of the ciliary body. Co-labelling with cell markers revealed that the BrdU positive cells also showed nestin expression but not glial fibrillary acidic protein (GFAP). Conclusions The results illustrate that proliferation of retinal progenitor cells situated in ciliary body are subjected to regulation by selective serotonin reuptake inhibitors (SSRI) and corticosteroid, which is similar to our previous findings in neurogenic regions in central nervous system (CNS). Paroxetine treatment could reverse the suppressive effect of corticosterone on ciliary body cell proliferation. This provides information for future investigation of retinal stem cell biology and potential treatment of retinal degenerative diseases.en_HK
dc.languageengen_HK
dc.publisherZhonghua Yixuehui. The Journal's web site is located at http://www.cmj.org/en_HK
dc.relation.ispartofChinese Medical Journalen_HK
dc.subject5-bromo-2-deoxyuridineen_HK
dc.subjectCell proliferationen_HK
dc.subjectCiliary bodyen_HK
dc.subjectCorticosteroneen_HK
dc.subjectNeurogenesisen_HK
dc.subjectParoxetineen_HK
dc.subject.meshAdrenal Glands - drug effects - pathology-
dc.subject.meshAnimals-
dc.subject.meshBody Weight - drug effects-
dc.subject.meshCell Proliferation - drug effects-
dc.subject.meshCiliary Body - cytology - drug effects-
dc.titleRoles of paroxetine and corticosterone on adult mammalian ciliary body cell proliferationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0366-6999&volume=123&issue=10&spage=1305&epage=1310&date=2010&atitle=Roles+of+paroxetine+and+corticosterone+on+adult+mammalian+retinal+ciliary+body+cell+proliferation-
dc.identifier.emailLee, T:tmclee@hku.hken_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.authorityLee, T=rp00564en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.3760/cma.j.issn.0366-6999.2010.10.015en_HK
dc.identifier.pmid20529586-
dc.identifier.scopuseid_2-s2.0-77952918338en_HK
dc.identifier.hkuros175833en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77952918338&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume123en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1305en_HK
dc.identifier.epage1310en_HK
dc.identifier.isiWOS:000278219900015-
dc.publisher.placeChinaen_HK
dc.identifier.scopusauthoridWang, H=15047316100en_HK
dc.identifier.scopusauthoridLau, BWM=21934562200en_HK
dc.identifier.scopusauthoridYau, SY=24330296200en_HK
dc.identifier.scopusauthoridLi, SY=24329630700en_HK
dc.identifier.scopusauthoridLeung, N=36519157200en_HK
dc.identifier.scopusauthoridWang, NL=7404340277en_HK
dc.identifier.scopusauthoridTang, SW=23968420300en_HK
dc.identifier.scopusauthoridLee, T=7501437381en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK

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