File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: A lack of contact of sperm with accessory sex gland secretions deregulates DNA methylation and imprinted gene expression in rodent embryos

TitleA lack of contact of sperm with accessory sex gland secretions deregulates DNA methylation and imprinted gene expression in rodent embryos
Authors
Issue Date2009
Citation
Systems Biology In Reproductive Medicine, 2009, v. 55 n. 5-6, p. 200-213 How to Cite?
AbstractIncreased oxidative DNA damage is observed in sperm devoid of contact with accessory sex gland (ASG) secretion. After fertilization, these sperm may produce abnormal embryos. In this study, we investigated the possibility that the pattern of DNA methylation and imprinted gene expression in these embryos may be perturbed. Epididymal sperm, uterine sperm, and embryonic day 13 (E13) embryos were collected from hamster and mouse. The extent of global DNA methylation was determined with an antibody against methylcytosine using an embryo DNA dot. The sperm and embryo Gtl2 promoter and H19 differential methylated region (DMR) were subject to bisulfite sequencing. Expression of their reciprocally activated genes Dlk1 and Igf2 was quantified by real-time PCR. Genome-wide DNA hypo-methylation in both hamster and mouse embryos sired by males without ASG was observed. The imprinting pattern of fetal mouse Gtl2 promoter and fetal hamster H19 DMR were also disrupted while the expression of Dlk1 and Igf2 was dysregulated in the hamster embryo. This study suggests that a lack of contact of sperm with the ASG secretion disrupts genome-wide DNA methylation and also affects the DNA methylation pattern of imprinted genes in embryos. © 2009 Informa UK Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/124501
ISSN
2015 SCImago Journal Rankings: 0.510
ISI Accession Number ID
Funding AgencyGrant Number
Research Grant Council of the Hong Kong Special Administrative RegionCLTHK4419/03
Funding Information:

We thank Dr. Anne C. Ferguson-Smith of the Department of Anatomy at the University of Cambridge for her invaluable contribution in formulating this research. This work was supported by a Competitive Earn-lark Grant (CLTHK4419/03) from the Research Grant Council of the Hong Kong Special Administrative Region.

References

 

DC FieldValueLanguage
dc.contributor.authorPoon, HKen_HK
dc.contributor.authorLee, KHen_HK
dc.contributor.authorWong, CLen_HK
dc.contributor.authorO, WSen_HK
dc.contributor.authorChow, PHen_HK
dc.date.accessioned2010-10-31T10:37:56Z-
dc.date.available2010-10-31T10:37:56Z-
dc.date.issued2009en_HK
dc.identifier.citationSystems Biology In Reproductive Medicine, 2009, v. 55 n. 5-6, p. 200-213en_HK
dc.identifier.issn1939-6376en_HK
dc.identifier.urihttp://hdl.handle.net/10722/124501-
dc.description.abstractIncreased oxidative DNA damage is observed in sperm devoid of contact with accessory sex gland (ASG) secretion. After fertilization, these sperm may produce abnormal embryos. In this study, we investigated the possibility that the pattern of DNA methylation and imprinted gene expression in these embryos may be perturbed. Epididymal sperm, uterine sperm, and embryonic day 13 (E13) embryos were collected from hamster and mouse. The extent of global DNA methylation was determined with an antibody against methylcytosine using an embryo DNA dot. The sperm and embryo Gtl2 promoter and H19 differential methylated region (DMR) were subject to bisulfite sequencing. Expression of their reciprocally activated genes Dlk1 and Igf2 was quantified by real-time PCR. Genome-wide DNA hypo-methylation in both hamster and mouse embryos sired by males without ASG was observed. The imprinting pattern of fetal mouse Gtl2 promoter and fetal hamster H19 DMR were also disrupted while the expression of Dlk1 and Igf2 was dysregulated in the hamster embryo. This study suggests that a lack of contact of sperm with the ASG secretion disrupts genome-wide DNA methylation and also affects the DNA methylation pattern of imprinted genes in embryos. © 2009 Informa UK Ltd.en_HK
dc.languageengen_HK
dc.relation.ispartofSystems Biology in Reproductive Medicineen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCongenital Abnormalities - embryologyen_HK
dc.subject.meshCricetinaeen_HK
dc.subject.meshDNA Methylation - drug effectsen_HK
dc.subject.meshEmbryo, Mammalian - metabolismen_HK
dc.subject.meshEmbryonic Development - geneticsen_HK
dc.subject.meshGene Expression Regulation, Developmentalen_HK
dc.subject.meshGenitalia, Male - secretionen_HK
dc.subject.meshGenomic Imprinting - drug effectsen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiceen_HK
dc.subject.meshProstatectomyen_HK
dc.subject.meshProteins - geneticsen_HK
dc.subject.meshSpermatozoa - physiologyen_HK
dc.titleA lack of contact of sperm with accessory sex gland secretions deregulates DNA methylation and imprinted gene expression in rodent embryosen_HK
dc.typeArticleen_HK
dc.identifier.emailO, WS:owaisum@hkucc.hku.hken_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3109/19396360903165256en_HK
dc.identifier.pmid19938955-
dc.identifier.scopuseid_2-s2.0-72949105311en_HK
dc.identifier.hkuros172947en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-72949105311&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume55en_HK
dc.identifier.issue5-6en_HK
dc.identifier.spage200en_HK
dc.identifier.epage213en_HK
dc.identifier.isiWOS:000272975100005-
dc.identifier.scopusauthoridPoon, HK=7007103075en_HK
dc.identifier.scopusauthoridLee, KH=7501505976en_HK
dc.identifier.scopusauthoridWong, CL=26535204400en_HK
dc.identifier.scopusauthoridO, WS=6701729369en_HK
dc.identifier.scopusauthoridChow, PH=7202656919en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats