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Article: Bauhinia variegata var. variegata trypsin inhibitor: From isolation to potential medicinal applications
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TitleBauhinia variegata var. variegata trypsin inhibitor: From isolation to potential medicinal applications
 
AuthorsFang, EF3
Wong, JH3
Bah, CSF2
Lin, P3
Tsao, SW1
Ng, TB3
 
KeywordsBauhinia variegata var. variegata
Cytokines
HIV
Nasopharyngeal carcinoma
Proteinase inhibitors
 
Issue Date2010
 
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
 
CitationBiochemical And Biophysical Research Communications, 2010, v. 396 n. 4, p. 806-811 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.bbrc.2010.04.140
 
AbstractHere we report for the first time of a new Kunitz-type trypsin inhibitor (termed BvvTI) from seeds of the Camel's foot tree, Bauhinia variegata var. variegata. BvvTI shares the same reactive site residues (Arg, Ser) and exhibits a homology of N-terminal amino acid sequence to other Bauhinia protease inhibitors. The trypsin inhibitory activity (K i, 0.1 × 10 -9 M) of BvvTI ranks the highest among them. Besides anti-HIV-1 reverse transcriptase activity, BvvTI could significantly inhibit the proliferation of nasopharyngeal cancer CNE-1 cells in a selective way. This may partially be contributed by its induction of cytokines and apoptotic bodies. These results unveil potential medicinal applications of BvvTI. © 2010 Elsevier Inc. All rights reserved.
 
ISSN0006-291X
2012 Impact Factor: 2.406
2012 SCImago Journal Rankings: 1.049
 
DOIhttp://dx.doi.org/10.1016/j.bbrc.2010.04.140
 
ISI Accession Number IDWOS:000279125100005
Funding AgencyGrant Number
School of Biological Sciences, the University of Hong Kong
Funding Information:

We thank Dr. Carol P.Y. Lau, Prof. Lawrence Ramsden and Prof. Richard M.K. Saunders from School of Biological Sciences, the University of Hong Kong, for their kind support of the project.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorFang, EF
 
dc.contributor.authorWong, JH
 
dc.contributor.authorBah, CSF
 
dc.contributor.authorLin, P
 
dc.contributor.authorTsao, SW
 
dc.contributor.authorNg, TB
 
dc.date.accessioned2010-10-31T10:37:50Z
 
dc.date.available2010-10-31T10:37:50Z
 
dc.date.issued2010
 
dc.description.abstractHere we report for the first time of a new Kunitz-type trypsin inhibitor (termed BvvTI) from seeds of the Camel's foot tree, Bauhinia variegata var. variegata. BvvTI shares the same reactive site residues (Arg, Ser) and exhibits a homology of N-terminal amino acid sequence to other Bauhinia protease inhibitors. The trypsin inhibitory activity (K i, 0.1 × 10 -9 M) of BvvTI ranks the highest among them. Besides anti-HIV-1 reverse transcriptase activity, BvvTI could significantly inhibit the proliferation of nasopharyngeal cancer CNE-1 cells in a selective way. This may partially be contributed by its induction of cytokines and apoptotic bodies. These results unveil potential medicinal applications of BvvTI. © 2010 Elsevier Inc. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationBiochemical And Biophysical Research Communications, 2010, v. 396 n. 4, p. 806-811 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.bbrc.2010.04.140
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.bbrc.2010.04.140
 
dc.identifier.epage811
 
dc.identifier.hkuros182821
 
dc.identifier.isiWOS:000279125100005
Funding AgencyGrant Number
School of Biological Sciences, the University of Hong Kong
Funding Information:

We thank Dr. Carol P.Y. Lau, Prof. Lawrence Ramsden and Prof. Richard M.K. Saunders from School of Biological Sciences, the University of Hong Kong, for their kind support of the project.

 
dc.identifier.issn0006-291X
2012 Impact Factor: 2.406
2012 SCImago Journal Rankings: 1.049
 
dc.identifier.issue4
 
dc.identifier.openurl
 
dc.identifier.pmid20435016
 
dc.identifier.scopuseid_2-s2.0-77953288202
 
dc.identifier.spage806
 
dc.identifier.urihttp://hdl.handle.net/10722/124499
 
dc.identifier.volume396
 
dc.languageeng
 
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
 
dc.publisher.placeUnited States
 
dc.relation.ispartofBiochemical and Biophysical Research Communications
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnti-HIV Agents - chemistry - isolation and purification - pharmacology
 
dc.subject.meshAntineoplastic Agents - chemistry - isolation and purification - pharmacology
 
dc.subject.meshBauhinia - metabolism
 
dc.subject.meshPeptides - chemistry - isolation and purification - pharmacology
 
dc.subject.meshPlant Proteins - chemistry - isolation and purification - pharmacology
 
dc.subjectBauhinia variegata var. variegata
 
dc.subjectCytokines
 
dc.subjectHIV
 
dc.subjectNasopharyngeal carcinoma
 
dc.subjectProteinase inhibitors
 
dc.titleBauhinia variegata var. variegata trypsin inhibitor: From isolation to potential medicinal applications
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. University of Otago
  3. Chinese University of Hong Kong