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Article: Coexpression of adrenomedullin and its receptors in the reproductive system of the rat: effects on steroid secretion in rat ovary

TitleCoexpression of adrenomedullin and its receptors in the reproductive system of the rat: effects on steroid secretion in rat ovary
Authors
KeywordsEstradiol
Follicle-stimulating hormone
Human chorionic gonadotrophin
Ovary
Progesterone
Issue Date2009
PublisherRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR). The Journal's web site is located at http://www.celljournal.org/
Citation
Cell Journal (Yakhteh), 2009, v. 11 Suppl. 1 , p. 36 How to Cite?
AbstractObjective: The expression of adrenomedullin (ADM) in the reproductive system of the female rat and its effect on the secretion of estradiol and progesterone was studied. Materials and Methods: In adult Sprague-Dawley rats gene expression of ADM and its receptor components in the ovarian follicles oviduct were analyzed by real-time PCR (qPCR). Ovarian follicles and corpora lutea isolated from stimulated immature rats were cultured to study the effects ADM on in vitro basal FSH-stimulated estradiol secretion or eCG stimulated progesterone secretion. Results: Ovarian ADM and Adm mRNA levels were decreased at estrus whereas oviductal Adm mRNA levels were low at proestrus. Both tissues were shown to coexpress mRNAs encoding the calcitonin receptor-like receptor and receptor activity-modifying protein 1 (Ramp1) Ramp2 and Ramp3. Gel filtration chromatography of ovarian extracts showed two peaks with the predominant one eluting at the position of authentic rat ADM(1-50) at estrus and at the position of ADM precursor at diestrus. Positive ADM immunostaining was localized in the granulosa and theca cells of the follicle and corpora lutea of the ovary. ADM inhibited FSH-induced estradiol secretion in 2-day-old follicles and also suppressed eCG-stimulated progesterone release in the corpora lutea was abolished by calcitonin gene-related peptide (8-37) and ADM (22-52) respectively. Conclusion: The presence of ADM and the gene expression of ADM and its receptor components in the female reproductive system suggest a paracine effect of ADM on ovarian steroidogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/124497
ISSN
2015 Impact Factor: 1.275
2015 SCImago Journal Rankings: 0.485

 

DC FieldValueLanguage
dc.contributor.authorLi, Len_HK
dc.contributor.authorHwang, ISSen_HK
dc.contributor.authorTang, Fen_HK
dc.contributor.authorO, WSen_HK
dc.contributor.authorLi, YYen_HK
dc.date.accessioned2010-10-31T10:37:43Z-
dc.date.available2010-10-31T10:37:43Z-
dc.date.issued2009en_HK
dc.identifier.citationCell Journal (Yakhteh), 2009, v. 11 Suppl. 1 , p. 36en_HK
dc.identifier.issn2228-5806-
dc.identifier.urihttp://hdl.handle.net/10722/124497-
dc.description.abstractObjective: The expression of adrenomedullin (ADM) in the reproductive system of the female rat and its effect on the secretion of estradiol and progesterone was studied. Materials and Methods: In adult Sprague-Dawley rats gene expression of ADM and its receptor components in the ovarian follicles oviduct were analyzed by real-time PCR (qPCR). Ovarian follicles and corpora lutea isolated from stimulated immature rats were cultured to study the effects ADM on in vitro basal FSH-stimulated estradiol secretion or eCG stimulated progesterone secretion. Results: Ovarian ADM and Adm mRNA levels were decreased at estrus whereas oviductal Adm mRNA levels were low at proestrus. Both tissues were shown to coexpress mRNAs encoding the calcitonin receptor-like receptor and receptor activity-modifying protein 1 (Ramp1) Ramp2 and Ramp3. Gel filtration chromatography of ovarian extracts showed two peaks with the predominant one eluting at the position of authentic rat ADM(1-50) at estrus and at the position of ADM precursor at diestrus. Positive ADM immunostaining was localized in the granulosa and theca cells of the follicle and corpora lutea of the ovary. ADM inhibited FSH-induced estradiol secretion in 2-day-old follicles and also suppressed eCG-stimulated progesterone release in the corpora lutea was abolished by calcitonin gene-related peptide (8-37) and ADM (22-52) respectively. Conclusion: The presence of ADM and the gene expression of ADM and its receptor components in the female reproductive system suggest a paracine effect of ADM on ovarian steroidogenesis.-
dc.languageengen_HK
dc.publisherRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR). The Journal's web site is located at http://www.celljournal.org/-
dc.relation.ispartofCell Journal (Yakhteh)en_HK
dc.subjectEstradiol-
dc.subjectFollicle-stimulating hormone-
dc.subjectHuman chorionic gonadotrophin-
dc.subjectOvary-
dc.subjectProgesterone-
dc.titleCoexpression of adrenomedullin and its receptors in the reproductive system of the rat: effects on steroid secretion in rat ovaryen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=2228-5806&volume=11 Suppl. 1 &spage=36&epage=&date=2009&atitle=Coexpression+of+adrenomedullin+and+its+receptors+in+the+reproductive+system+of+the+rat:+effects+on+steroid+secretion+in+rat+ovary-
dc.identifier.emailLi, L: sarabirdlee@gmail.comen_HK
dc.identifier.emailHwang, ISS: isabelbelbel@hotmail.comen_HK
dc.identifier.emailTang, F: ftang@hkucc.hku.hken_HK
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_HK
dc.identifier.emailLi, YY: cyyli@graduate.hku.hken_HK
dc.identifier.authorityTang, F=rp00327en_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.identifier.hkuros172949en_HK
dc.identifier.volume11 Suppl. 1en_HK
dc.identifier.spage36en_HK

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