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Article: Inducible nucleosome depletion at OREBP-binding-sites by hypertonic stress
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TitleInducible nucleosome depletion at OREBP-binding-sites by hypertonic stress
 
AuthorsTong, EHY2 2 1
Guo, JJ3
Xu, SX2 2
Mak, K1
Chung, SK1
Chung, SSM1
Huang, AL3
Ko, BCB2 2
 
Issue Date2009
 
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
 
CitationPlos One, 2009, v. 4 n. 12 [How to Cite?]
DOI: http://dx.doi.org/10.1371/journal.pone.0008435
 
AbstractBackground: Osmotic Response Element-Binding Protein (OREBP), also known as TonEBP or NFAT5, is a unique transcription factor. It is hitherto the only known mammalian transcription factor that regulates hypertonic stress-induced gene transcription. In addition, unlike other monomeric members of the NFAT family, OREBP exists as a homodimer and it is the only transcription factor known to bind naked DNA targets by complete encirclement in vitro. Nevertheless, how OREBP interacts with target DNA, also known as ORE/TonE, and how it elicits gene transcription in vivo, remains unknown. Methodology: Using hypertonic induction of the aldose reductase (AR) gene activation as a model, we showed that OREs contained dynamic nucleosomes. Hypertonic stress induced a rapid and reversible loss of nucleosome(s) around the OREs. The loss of nucleosome(s) was found to be initiated by an OREBP-independent mechanism, but was significantly potentiated in the presence of OREBP. Furthermore, hypertonic induction of AR gene was associated with an OREBPdependent hyperacetylation of histones that spanned the 59 upstream sequences and at least some exons of the gene. Nevertheless, nucleosome loss was not regulated by the acetylation status of histone. Significance: Our findings offer novel insights into the mechanism of OREBP-dependent transcriptional regulation and provide a basis for understanding how histone eviction and transcription factor recruitment are coupled. © 2009 Tong et al.
 
ISSN1932-6203
2012 Impact Factor: 3.73
2012 SCImago Journal Rankings: 1.512
 
DOIhttp://dx.doi.org/10.1371/journal.pone.0008435
 
PubMed Central IDPMC2793017
 
ISI Accession Number IDWOS:000273104000005
Funding AgencyGrant Number
Research Grant CouncilCUHK 7327/04M
CUHK 466108
Chinese University of Hong Kong Direct2041317
Funding Information:

This work was supported by the Research Grant Council Grants CUHK 7327/04M, CUHK 466108 and by the Chinese University of Hong Kong Direct Grant for Research 2041317 ( to B. C. B. K). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorTong, EHY
 
dc.contributor.authorGuo, JJ
 
dc.contributor.authorXu, SX
 
dc.contributor.authorMak, K
 
dc.contributor.authorChung, SK
 
dc.contributor.authorChung, SSM
 
dc.contributor.authorHuang, AL
 
dc.contributor.authorKo, BCB
 
dc.date.accessioned2010-10-31T10:37:09Z
 
dc.date.available2010-10-31T10:37:09Z
 
dc.date.issued2009
 
dc.description.abstractBackground: Osmotic Response Element-Binding Protein (OREBP), also known as TonEBP or NFAT5, is a unique transcription factor. It is hitherto the only known mammalian transcription factor that regulates hypertonic stress-induced gene transcription. In addition, unlike other monomeric members of the NFAT family, OREBP exists as a homodimer and it is the only transcription factor known to bind naked DNA targets by complete encirclement in vitro. Nevertheless, how OREBP interacts with target DNA, also known as ORE/TonE, and how it elicits gene transcription in vivo, remains unknown. Methodology: Using hypertonic induction of the aldose reductase (AR) gene activation as a model, we showed that OREs contained dynamic nucleosomes. Hypertonic stress induced a rapid and reversible loss of nucleosome(s) around the OREs. The loss of nucleosome(s) was found to be initiated by an OREBP-independent mechanism, but was significantly potentiated in the presence of OREBP. Furthermore, hypertonic induction of AR gene was associated with an OREBPdependent hyperacetylation of histones that spanned the 59 upstream sequences and at least some exons of the gene. Nevertheless, nucleosome loss was not regulated by the acetylation status of histone. Significance: Our findings offer novel insights into the mechanism of OREBP-dependent transcriptional regulation and provide a basis for understanding how histone eviction and transcription factor recruitment are coupled. © 2009 Tong et al.
 
dc.description.naturepublished_or_final_version
 
dc.identifier.citationPlos One, 2009, v. 4 n. 12 [How to Cite?]
DOI: http://dx.doi.org/10.1371/journal.pone.0008435
 
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0008435
 
dc.identifier.hkuros182033
 
dc.identifier.isiWOS:000273104000005
Funding AgencyGrant Number
Research Grant CouncilCUHK 7327/04M
CUHK 466108
Chinese University of Hong Kong Direct2041317
Funding Information:

This work was supported by the Research Grant Council Grants CUHK 7327/04M, CUHK 466108 and by the Chinese University of Hong Kong Direct Grant for Research 2041317 ( to B. C. B. K). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

 
dc.identifier.issn1932-6203
2012 Impact Factor: 3.73
2012 SCImago Journal Rankings: 1.512
 
dc.identifier.issue12
 
dc.identifier.openurl
 
dc.identifier.pmcidPMC2793017
 
dc.identifier.pmid20041176
 
dc.identifier.scopuseid_2-s2.0-77949517845
 
dc.identifier.urihttp://hdl.handle.net/10722/124487
 
dc.identifier.volume4
 
dc.languageeng
 
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
 
dc.publisher.placeUnited States
 
dc.relation.ispartofPLoS ONE
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshAcetylation - drug effects
 
dc.subject.meshHypertonic Solutions - pharmacology
 
dc.subject.meshNFATC Transcription Factors - metabolism
 
dc.subject.meshNucleosomes - metabolism
 
dc.subject.meshStress, Physiological - drug effects
 
dc.titleInducible nucleosome depletion at OREBP-binding-sites by hypertonic stress
 
dc.typeArticle
 
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<contributor.author>Chung, SSM</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. Chinese University of Hong Kong
  3. Chongqing University of Medical Sciences