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Article: Regulation of retinal progenitor cell differentiation by bone morphogenetic protein 4 is mediated by the smad/id cascade

TitleRegulation of retinal progenitor cell differentiation by bone morphogenetic protein 4 is mediated by the smad/id cascade
Authors
Issue Date2010
PublisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
Citation
Investigative Ophthalmology And Visual Science, 2010, v. 51 n. 7, p. 3764-3773 How to Cite?
AbstractPurpose. Bone morphogenetic proteins (BMPs) are secreted signaling molecules that are implicated in the control of multiple events during mouse eye development. However, little is known about the mechanisms by which BMP signaling regulates these retinal developmental processes. Methods. Real-time PCR, Western blot, and immunohistochem-istry were used to investigate the expression of components of BMP signaling in the mouse retina. Retinal progenitor cells (RPCs) were used to study the effects of BMP4 on retinal cell differentiation and regulation of Id protein expression. Results. Results showed that BMP2, -4, and -7; BMP receptor (BMPRIb) mRNAs; and proteins and downstream signaling molecule Smad1/5/8 proteins were all highly expressed in the mouse retina during the embryonic (E13.5-E18.5) and early postnatal (P)1 stage and that the expression was downregu-lated in the adult. On stimulation with BMP4, cultured mouse RPCs differentiated into neuronal lineage whereas astrocyte cell differentiation was inhibited. BMP4 mainly stimulated production of retinal ganglion cells (RGCs). Results also revealed that BMPs and BMPRIb were co-localized with inhibitors of differentiation (Id) (mainly Id1 and -3) in RGCs in the adult mouse retina. Exposure of RPCs to BMP4 upregulated Id1-3 expression levels, mediated through the phosphorylation of Smad1/5/8 proteins. Conclusions. These results suggest that Id genes are one of the potential targets of BMP signaling in the differentiation of RPCs. © Association for Research in Vision and Ophthalmology.
Persistent Identifierhttp://hdl.handle.net/10722/124478
ISSN
2015 Impact Factor: 3.427
2015 SCImago Journal Rankings: 2.008
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong200611159203
Hong Kong Research Grants Council10208603
Funding Information:

Supported by Seed Funding Program for Basic Research Grant 200611159203 from the University of Hong Kong and General Research Fund Grant 10208603 from the Hong Kong Research Grants Council.

References

 

DC FieldValueLanguage
dc.contributor.authorDu, Yen_HK
dc.contributor.authorXiao, Qen_HK
dc.contributor.authorYip, HKen_HK
dc.date.accessioned2010-10-31T10:36:39Z-
dc.date.available2010-10-31T10:36:39Z-
dc.date.issued2010en_HK
dc.identifier.citationInvestigative Ophthalmology And Visual Science, 2010, v. 51 n. 7, p. 3764-3773en_HK
dc.identifier.issn0146-0404en_HK
dc.identifier.urihttp://hdl.handle.net/10722/124478-
dc.description.abstractPurpose. Bone morphogenetic proteins (BMPs) are secreted signaling molecules that are implicated in the control of multiple events during mouse eye development. However, little is known about the mechanisms by which BMP signaling regulates these retinal developmental processes. Methods. Real-time PCR, Western blot, and immunohistochem-istry were used to investigate the expression of components of BMP signaling in the mouse retina. Retinal progenitor cells (RPCs) were used to study the effects of BMP4 on retinal cell differentiation and regulation of Id protein expression. Results. Results showed that BMP2, -4, and -7; BMP receptor (BMPRIb) mRNAs; and proteins and downstream signaling molecule Smad1/5/8 proteins were all highly expressed in the mouse retina during the embryonic (E13.5-E18.5) and early postnatal (P)1 stage and that the expression was downregu-lated in the adult. On stimulation with BMP4, cultured mouse RPCs differentiated into neuronal lineage whereas astrocyte cell differentiation was inhibited. BMP4 mainly stimulated production of retinal ganglion cells (RGCs). Results also revealed that BMPs and BMPRIb were co-localized with inhibitors of differentiation (Id) (mainly Id1 and -3) in RGCs in the adult mouse retina. Exposure of RPCs to BMP4 upregulated Id1-3 expression levels, mediated through the phosphorylation of Smad1/5/8 proteins. Conclusions. These results suggest that Id genes are one of the potential targets of BMP signaling in the differentiation of RPCs. © Association for Research in Vision and Ophthalmology.en_HK
dc.languageengen_HK
dc.publisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.orgen_HK
dc.relation.ispartofInvestigative Ophthalmology and Visual Scienceen_HK
dc.titleRegulation of retinal progenitor cell differentiation by bone morphogenetic protein 4 is mediated by the smad/id cascadeen_HK
dc.typeArticleen_HK
dc.identifier.emailYip, HK:hkfyip@hku.hken_HK
dc.identifier.authorityYip, HK=rp00285en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1167/iovs.09-4906en_HK
dc.identifier.pmid20130285-
dc.identifier.scopuseid_2-s2.0-77955912864en_HK
dc.identifier.hkuros176341en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77955912864&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume51en_HK
dc.identifier.issue7en_HK
dc.identifier.spage3764en_HK
dc.identifier.epage3773en_HK
dc.identifier.eissn1552-5783-
dc.identifier.isiWOS:000279047500059-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridDu, Y=36993717500en_HK
dc.identifier.scopusauthoridXiao, Q=35621162200en_HK
dc.identifier.scopusauthoridYip, HK=7101980864en_HK

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