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Article: Stem-like cancer cells are inducible by increasing genomic instability in cancer cells

TitleStem-like cancer cells are inducible by increasing genomic instability in cancer cells
Authors
Issue Date2010
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 2010, v. 285 n. 7, p. 4931-4940 How to Cite?
Abstract
The existence of cancer stem cells (CSCs) or stem-like cancer cells (SLCCs) is regarded as the cause of tumor formation and recurrence. However, the origin of such cells remains controversial with two competing hypotheses:CSCsare either transformed from tissue adult stem cells or dedifferentiated from transformed progenitor cells. Compelling evidence has determined the chromosomal aneuploidy to be one of the hallmarks of cancer cells, indicating genome instability plays an important role in tumorigenesis, for which CSCs are believed to be the initiator. To gain direct evidence that genomic instability is involved in the induction of SLCCs, we utilized multiple approaches to enhance genomic instability and monitored the percentage of SLCC in cultured cancer cells. Using side population (SP) cells as a marker for SLCC in human nasopharyngeal carcinoma (NPC) and CD133 for human neuroblastoma cells,wefound thatDNAdamageinducers,UVand mitomycin C were capable of increasing SP cells in NPCCNE-2 and neuroblastoma SKN-SH cells. Likewise, either overexpression of a key regulator of cell cycle, Mad2, or knock down of Aurora B, an important kinase in mitosis, or Cdh1, a key E3 ligase in cell cycle, resulted in a significant increase of SP cells in CNE-2. More interestingly, enrichment of SP cells was observed in recurrent tumor tissues as compared with the primary tumor in the same NPC patients. Our study thus suggested that, beside transformation of tissue stem cells leading to CSC generation, genomic instability could be another potential mechanism resulting in SLCC formation, especially at tumor recurrence stage. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/124474
ISSN
2013 Impact Factor: 4.600
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Chinese National 973 Program2006CB910104
2010CB912201
Chinese National 863 Program2006AA02A404
Guangdong Province-National Natural Science Foundation of Chinau0732005
Funding Information:

The work was supported by the Chinese National 973 Program (Grants 2006CB910104 and 2010CB912201), the Chinese National 863 Program (Grant 2006AA02A404), and the Guangdong Province-National Natural Science Foundation of China Cooperation Program (Grant u0732005).

References

 

Author Affiliations
  1. Sun Yat-Sen University Cancer Center
  2. The University of Hong Kong
  3. Guangzhou Institute of Biomedicine and Health
  4. Sun Yat-Sen University
DC FieldValueLanguage
dc.contributor.authorLiang, Yen_HK
dc.contributor.authorZhong, Zen_HK
dc.contributor.authorHuang, Yen_HK
dc.contributor.authorDeng, Wen_HK
dc.contributor.authorCao, Jen_HK
dc.contributor.authorTsao, Gen_HK
dc.contributor.authorLiu, Qen_HK
dc.contributor.authorPei, Den_HK
dc.contributor.authorKang, Ten_HK
dc.contributor.authorZeng, YXen_HK
dc.date.accessioned2010-10-31T10:36:26Z-
dc.date.available2010-10-31T10:36:26Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Biological Chemistry, 2010, v. 285 n. 7, p. 4931-4940en_HK
dc.identifier.issn0021-9258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/124474-
dc.description.abstractThe existence of cancer stem cells (CSCs) or stem-like cancer cells (SLCCs) is regarded as the cause of tumor formation and recurrence. However, the origin of such cells remains controversial with two competing hypotheses:CSCsare either transformed from tissue adult stem cells or dedifferentiated from transformed progenitor cells. Compelling evidence has determined the chromosomal aneuploidy to be one of the hallmarks of cancer cells, indicating genome instability plays an important role in tumorigenesis, for which CSCs are believed to be the initiator. To gain direct evidence that genomic instability is involved in the induction of SLCCs, we utilized multiple approaches to enhance genomic instability and monitored the percentage of SLCC in cultured cancer cells. Using side population (SP) cells as a marker for SLCC in human nasopharyngeal carcinoma (NPC) and CD133 for human neuroblastoma cells,wefound thatDNAdamageinducers,UVand mitomycin C were capable of increasing SP cells in NPCCNE-2 and neuroblastoma SKN-SH cells. Likewise, either overexpression of a key regulator of cell cycle, Mad2, or knock down of Aurora B, an important kinase in mitosis, or Cdh1, a key E3 ligase in cell cycle, resulted in a significant increase of SP cells in CNE-2. More interestingly, enrichment of SP cells was observed in recurrent tumor tissues as compared with the primary tumor in the same NPC patients. Our study thus suggested that, beside transformation of tissue stem cells leading to CSC generation, genomic instability could be another potential mechanism resulting in SLCC formation, especially at tumor recurrence stage. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_HK
dc.relation.ispartofJournal of Biological Chemistryen_HK
dc.rightsJournal of Biological Chemistry. Copyright © American Society for Biochemistry and Molecular Biology, Inc.-
dc.rightsThis research was originally published in [Journal Name]. Author(s). Title. Journal Name. Year. Vol:pp-pp. © the American Society for Biochemistry and Molecular Biology-
dc.subject.meshATP-Binding Cassette Transporters - metabolism-
dc.subject.meshAntigens, CD - metabolism-
dc.subject.meshBlotting, Western-
dc.subject.meshGenomic Instability - genetics - physiology-
dc.subject.meshNeoplastic Stem Cells - cytology - metabolism-
dc.titleStem-like cancer cells are inducible by increasing genomic instability in cancer cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9258&volume=285&issue=7&spage=4931&epage=4940&date=2010&atitle=Stem-like+cancer+cells+are+inducible+by+increasing+genomic+instability+in+cancer+cells-
dc.identifier.emailDeng, W: wdeng@hkucc.hku.hken_HK
dc.identifier.emailTsao, G: gswtsao@hku.hken_HK
dc.identifier.authorityDeng, W=rp01640en_HK
dc.identifier.authorityTsao, G=rp00399en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1074/jbc.M109.048397en_HK
dc.identifier.pmid20007324en_HK
dc.identifier.pmcidPMC2836097-
dc.identifier.scopuseid_2-s2.0-77951192102en_HK
dc.identifier.hkuros182598en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77951192102&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume285en_HK
dc.identifier.issue7en_HK
dc.identifier.spage4931en_HK
dc.identifier.epage4940en_HK
dc.identifier.isiWOS:000275274800070-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLiang, Y=26421277700en_HK
dc.identifier.scopusauthoridZhong, Z=41763173300en_HK
dc.identifier.scopusauthoridHuang, Y=8977779900en_HK
dc.identifier.scopusauthoridDeng, W=7202223673en_HK
dc.identifier.scopusauthoridCao, J=14520965900en_HK
dc.identifier.scopusauthoridTsao, G=7102813116en_HK
dc.identifier.scopusauthoridLiu, Q=12809604100en_HK
dc.identifier.scopusauthoridPei, D=7102806599en_HK
dc.identifier.scopusauthoridKang, T=7402965550en_HK
dc.identifier.scopusauthoridZeng, YX=7402981579en_HK
dc.identifier.citeulike11284570-

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