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Article: Chromosome 3p12.3-p14.2 and 3q26.2-q26.32 are genomic markers for prognosis of advanced nasopharyngeal carcinoma

TitleChromosome 3p12.3-p14.2 and 3q26.2-q26.32 are genomic markers for prognosis of advanced nasopharyngeal carcinoma
Authors
Issue Date2009
PublisherAmerican Association for Cancer Research.
Citation
Cancer Epidemiology Biomarkers And Prevention, 2009, v. 18 n. 10, p. 2709-2716 How to Cite?
AbstractPurpose: Nasopharyngeal carcinoma is an epithelial malignancy with a remarkable racial and geographic distribution. Previous cytogenetic studies have shown nasopharyngeal carcinoma to be characterized by gross genomic aberrations. However, identification of susceptible gene loci in advanced nasopharyngeal carcinoma has been poorly discussed. Experimental Design: A genome-wide survey of gene copy number changes was initiated with two nasopharyngeal carcinoma cell lines by array-based comparative genomic hybridization analysis. These alterations were confirmed by a parallel analysis with the data from the gene expression microarray and were validated by quantitative PCR. Clinical association of the defined target genes was analyzed by fluorescence in situ hybridization on 48 metastatic tumors. Results: A high percentage of genes were consistently altered in dosage and expression levels with gain on 3q26.2-q26.32 and losses on 3p12.3-p14.2 and 9p21.3-p23. Six candidate genes, GPR160 (3q26.2-q27), SKIL (3q26), ADAMTS9 (3p14.2-p14.3), LRIG1 (3p14), MPDZ (9p22-p24), and ADFP (9p22.1) were validated by quantitative PCR. Fluorescence in situ hybridization studies revealed amplification of GPR160 (in 25% of cases) and SKIL (33%); and deletion of ADAMTS9 (30%), LRIG1 (35%), MPDZ (15%), and ADFP (15%). Clinical association analyses indicated a poor survival rate with genetic alterations at the defined 3p deletion (P = 0.0012) and the 3q amplification regions (P = 0.0114). Conclusion: The combined microarray technologies suggested novel candidate oncogenes, amplification of GPR160 and SKIL at 3q26.2-q26.32, and deletion of tumor suppressor genes ADAMTS9 and LRIG1 at 3p12.3-p14.2. Altered expression of these genes may be responsible for malignant progression and could be used as potential markers for nasopharyngeal carcinoma. Copyright © 2009 American Association for Cancer Research.
Persistent Identifierhttp://hdl.handle.net/10722/124473
ISSN
2015 Impact Factor: 3.622
2015 SCImago Journal Rankings: 2.579
ISI Accession Number ID
Funding AgencyGrant Number
National Health Research Institutes97A1CAPP18-014
98A1CAPP13-014
China Medical University HospitalCMU96-269
National Science Council, TaiwanNSC96-3112-B400-010
NSC97-3112-B400-007
Funding Information:

Grants from National Health Research Institutes (97A1CAPP18-014; 98A1CAPP13-014), China Medical University Hospital (CMU96-269), and the National Science Council, Taiwan (NSC96-3112-B400-010; NSC97-3112-B400-007).

References

 

DC FieldValueLanguage
dc.contributor.authorSheu, JJCen_HK
dc.contributor.authorLee, CHen_HK
dc.contributor.authorKo, JYen_HK
dc.contributor.authorTsao, GSWen_HK
dc.contributor.authorWu, CCen_HK
dc.contributor.authorFang, CYen_HK
dc.contributor.authorTsai, FJen_HK
dc.contributor.authorHua, CHen_HK
dc.contributor.authorChen, CLen_HK
dc.contributor.authorChen, JYen_HK
dc.date.accessioned2010-10-31T10:36:23Z-
dc.date.available2010-10-31T10:36:23Z-
dc.date.issued2009en_HK
dc.identifier.citationCancer Epidemiology Biomarkers And Prevention, 2009, v. 18 n. 10, p. 2709-2716en_HK
dc.identifier.issn1055-9965en_HK
dc.identifier.urihttp://hdl.handle.net/10722/124473-
dc.description.abstractPurpose: Nasopharyngeal carcinoma is an epithelial malignancy with a remarkable racial and geographic distribution. Previous cytogenetic studies have shown nasopharyngeal carcinoma to be characterized by gross genomic aberrations. However, identification of susceptible gene loci in advanced nasopharyngeal carcinoma has been poorly discussed. Experimental Design: A genome-wide survey of gene copy number changes was initiated with two nasopharyngeal carcinoma cell lines by array-based comparative genomic hybridization analysis. These alterations were confirmed by a parallel analysis with the data from the gene expression microarray and were validated by quantitative PCR. Clinical association of the defined target genes was analyzed by fluorescence in situ hybridization on 48 metastatic tumors. Results: A high percentage of genes were consistently altered in dosage and expression levels with gain on 3q26.2-q26.32 and losses on 3p12.3-p14.2 and 9p21.3-p23. Six candidate genes, GPR160 (3q26.2-q27), SKIL (3q26), ADAMTS9 (3p14.2-p14.3), LRIG1 (3p14), MPDZ (9p22-p24), and ADFP (9p22.1) were validated by quantitative PCR. Fluorescence in situ hybridization studies revealed amplification of GPR160 (in 25% of cases) and SKIL (33%); and deletion of ADAMTS9 (30%), LRIG1 (35%), MPDZ (15%), and ADFP (15%). Clinical association analyses indicated a poor survival rate with genetic alterations at the defined 3p deletion (P = 0.0012) and the 3q amplification regions (P = 0.0114). Conclusion: The combined microarray technologies suggested novel candidate oncogenes, amplification of GPR160 and SKIL at 3q26.2-q26.32, and deletion of tumor suppressor genes ADAMTS9 and LRIG1 at 3p12.3-p14.2. Altered expression of these genes may be responsible for malignant progression and could be used as potential markers for nasopharyngeal carcinoma. Copyright © 2009 American Association for Cancer Research.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research.-
dc.relation.ispartofCancer Epidemiology Biomarkers and Preventionen_HK
dc.subject.meshCell Line, Tumor-
dc.subject.meshChromosomes, Human, Pair 3-
dc.subject.meshGene Amplification-
dc.subject.meshGene Dosage-
dc.subject.meshNasopharyngeal Neoplasms - genetics - pathology-
dc.titleChromosome 3p12.3-p14.2 and 3q26.2-q26.32 are genomic markers for prognosis of advanced nasopharyngeal carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1055-9965&volume=18&issue=10&spage=2709&epage=2716&date=2009&atitle=Chromosome+3p12.3-p14.2+and+3q26.2-q26.32+are+genomic+markers+for+prognosis+of+advanced+nasopharyngeal+carcinoma-
dc.identifier.emailTsao, GSW:gswtsao@hkucc.hku.hken_HK
dc.identifier.authorityTsao, GSW=rp00399en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1158/1055-9965.EPI-09-0349en_HK
dc.identifier.pmid19815638-
dc.identifier.scopuseid_2-s2.0-70350091293en_HK
dc.identifier.hkuros182652en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70350091293&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume18en_HK
dc.identifier.issue10en_HK
dc.identifier.spage2709en_HK
dc.identifier.epage2716en_HK
dc.identifier.isiWOS:000270702100019-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSheu, JJC=7201928645en_HK
dc.identifier.scopusauthoridLee, CH=35620637800en_HK
dc.identifier.scopusauthoridKo, JY=7402678719en_HK
dc.identifier.scopusauthoridTsao, GSW=7102813116en_HK
dc.identifier.scopusauthoridWu, CC=7501671810en_HK
dc.identifier.scopusauthoridFang, CY=17342310200en_HK
dc.identifier.scopusauthoridTsai, FJ=7202660170en_HK
dc.identifier.scopusauthoridHua, CH=15127543000en_HK
dc.identifier.scopusauthoridChen, CL=7501965241en_HK
dc.identifier.scopusauthoridChen, JY=26037019400en_HK

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