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Article: Interaction between spinal opioid and adenosine receptors in remote cardiac preconditioning: effect of intrathecal morphine

TitleInteraction between spinal opioid and adenosine receptors in remote cardiac preconditioning: effect of intrathecal morphine
Authors
Issue Date2011
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/jvca
Citation
Journal of Cardiothoracic and Vascular Anesthesia, 2011, v. 25 n. 3, p. 444-448 How to Cite?
AbstractOBJECTIVES: Intrathecal morphine is cardioprotective and also triggers spinal adenosine release. This study investigated the role of spinal and peripheral adenosine receptors in intrathecal morphine cardioprotection. DESIGN: A randomized, prospective study. SETTING: A university research laboratory. PARTICIPANTS: Seventy-two male Sprague-Dawley rats. INTERVENTIONS: Anesthetized, open-chest, male Sprague-Dawley rats were assigned to 1 of 10 treatment groups 3 days after intrathecal catheter placement. Intrathecal morphine cardioprotection was induced with 3 mug/kg of morphine. Intrathecal normal saline was used as the control. The adenosine-receptor antagonist 8-(p-sulfophenyl) theophylline (50 mug/kg or 7.5 mg/kg) was given via intrathecal or intravenous routes, respectively, either 10 minutes before or immediately after morphine or saline. Ischemia reperfusion injury then was induced by 30 minutes of left coronary artery occlusion followed by 120 minutes of reperfusion. MEASUREMENTS AND MAIN RESULTS: Infarct size, as a percentage of the area at risk, was determined by 2,3,5-triphenyltetrazolium chloride staining. This was reduced significantly in the morphine group (25% +/- 5%) compared with the control (58% +/- 3%, p < 0.05). The addition of intravenous 8-SPT either before or after morphine significantly attenuated the cardioprotective effect. In comparison, intrathecal administration of 8-(p-sulfophenyl) theophylline before but not after morphine attenuated the cardioprotective effects of intrathecal morphine. CONCLUSIONS: Both central and peripheral adenosine receptors are involved in the signaling of intrathecal morphine preconditioning. Central receptors are important in the initiation of the process, whereas peripheral receptors have a role in ongoing mediation of the protective effect. © 2011 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/124467
ISSN
2015 Impact Factor: 1.519
2015 SCImago Journal Rankings: 0.646
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYao, Len_HK
dc.contributor.authorWong, GTCen_HK
dc.contributor.authorXia, Zen_HK
dc.contributor.authorIrwin, MGen_HK
dc.date.accessioned2010-10-31T10:35:59Z-
dc.date.available2010-10-31T10:35:59Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal of Cardiothoracic and Vascular Anesthesia, 2011, v. 25 n. 3, p. 444-448en_HK
dc.identifier.issn1053-0770-
dc.identifier.urihttp://hdl.handle.net/10722/124467-
dc.description.abstractOBJECTIVES: Intrathecal morphine is cardioprotective and also triggers spinal adenosine release. This study investigated the role of spinal and peripheral adenosine receptors in intrathecal morphine cardioprotection. DESIGN: A randomized, prospective study. SETTING: A university research laboratory. PARTICIPANTS: Seventy-two male Sprague-Dawley rats. INTERVENTIONS: Anesthetized, open-chest, male Sprague-Dawley rats were assigned to 1 of 10 treatment groups 3 days after intrathecal catheter placement. Intrathecal morphine cardioprotection was induced with 3 mug/kg of morphine. Intrathecal normal saline was used as the control. The adenosine-receptor antagonist 8-(p-sulfophenyl) theophylline (50 mug/kg or 7.5 mg/kg) was given via intrathecal or intravenous routes, respectively, either 10 minutes before or immediately after morphine or saline. Ischemia reperfusion injury then was induced by 30 minutes of left coronary artery occlusion followed by 120 minutes of reperfusion. MEASUREMENTS AND MAIN RESULTS: Infarct size, as a percentage of the area at risk, was determined by 2,3,5-triphenyltetrazolium chloride staining. This was reduced significantly in the morphine group (25% +/- 5%) compared with the control (58% +/- 3%, p < 0.05). The addition of intravenous 8-SPT either before or after morphine significantly attenuated the cardioprotective effect. In comparison, intrathecal administration of 8-(p-sulfophenyl) theophylline before but not after morphine attenuated the cardioprotective effects of intrathecal morphine. CONCLUSIONS: Both central and peripheral adenosine receptors are involved in the signaling of intrathecal morphine preconditioning. Central receptors are important in the initiation of the process, whereas peripheral receptors have a role in ongoing mediation of the protective effect. © 2011 Elsevier Inc. All rights reserved.-
dc.languageengen_HK
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/jvca-
dc.relation.ispartofJournal of Cardiothoracic and Vascular Anesthesiaen_HK
dc.subject.meshInjections, Spinal-
dc.subject.meshIschemic Preconditioning, Myocardial - methods-
dc.subject.meshMorphine - administration and dosage - metabolism-
dc.subject.meshReceptors, Opioid - agonists - metabolism-
dc.subject.meshReceptors, Purinergic P1 - metabolism-
dc.titleInteraction between spinal opioid and adenosine receptors in remote cardiac preconditioning: effect of intrathecal morphineen_HK
dc.typeArticleen_HK
dc.identifier.emailYao, L: luyao_mz@163.comen_HK
dc.identifier.emailWong, GTC: gordon@hku.hken_HK
dc.identifier.emailXia, Z: zhengyuan_xia@yahoo.comen_HK
dc.identifier.emailIrwin, MG: mgirwin@hkucc.hku.hken_HK
dc.identifier.authorityWong, GTC=rp00523en_HK
dc.identifier.authorityXia, Z=rp00532en_HK
dc.identifier.authorityIrwin, MG=rp00390en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1053/j.jvca.2010.05.012-
dc.identifier.pmid20688538-
dc.identifier.scopuseid_2-s2.0-79957781315-
dc.identifier.hkuros174756en_HK
dc.identifier.hkuros203034-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79957781315&selection=ref&src=s&origin=recordpage-
dc.identifier.volume25-
dc.identifier.issue3-
dc.identifier.spage444en_HK
dc.identifier.epage448-
dc.identifier.isiWOS:000291412000010-
dc.publisher.placeUnited States-

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