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Article: Proteomics of ischemia/reperfusion injury in rat intestine with and without ischemic postconditioning

TitleProteomics of ischemia/reperfusion injury in rat intestine with and without ischemic postconditioning
Authors
Keywordsintestine
ischemia/reperfusion injury
ischemic postconditioning
proteomic
Issue Date2010
PublisherElsevier Inc.. The Journal's web site is located at http://www.elsevier.com/locate/jsre
Citation
Journal Of Surgical Research, 2010, v. 164 n. 1, p. e173-e180 How to Cite?
AbstractBackground: Intestinal ischemia/reperfusion (I/R) injury is a critical condition associated with high morbidity and mortality. Our previous study showed that ischemic postconditioning (IPo) protects the intestinal mucosa from I/R injury. However, the precise molecular mechanisms of this event remain poorly elucidated. The aim of this study was to investigate the differentially expressed proteins of intestinal mucosa after intestinal I/R with or without IPo, and to explore the potential mechanisms of intestinal I/R injury and the protective effect of IPo in relation to the differential proteins. Materials and Methods: Intestinal I/R injury was established by occluding the superior mesenteric artery (SMA) for 60min followed by 60min reperfusion. The rats were randomly allocated into one of three groups based upon the intervention (n = 8); sham : sham surgical preparation including isolation of the SMA without occlusion was performed; injury: there was no intervention either before or after SMA occlusion; IPo: three cycles of 30 s reperfusion-30 s reocclusion were imposed immediately upon reperfusion. A comparative proteomics approach with two-dimensional gel electrophoresis was used to isolate proteins in intestinal mucosa, the expression of which were regulated by I/R injury post-treated with or without IPo. The differentially displayed proteins were identified through matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). Results: Image analysis revealed that an average of 1300 protein spots were detected on each gel; 16 and 9 proteins showing more than 1.5-fold difference were identified between the Sham versus Injury group and injury group versus IPo group, respectively. The identified proteins were functionally involved in the cellular processes of energy metabolism, anti-oxidation, and anti-apoptosis. Conclusions: This study provided new clues for understanding the mechanisms of IPo against intestinal I/R injury. © 2010 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/124466
ISSN
2015 Impact Factor: 2.198
2015 SCImago Journal Rankings: 0.928
ISI Accession Number ID
Funding AgencyGrant Number
National Natural Science Foundation of China30672021
30872446
Funding Information:

The authors thank Dr. Liao Dong-jiang for technical help in proteomic analysis. This work was funded by a grant from National Natural Science Foundation of China (no. 30672021, 30872446 to KXL).

References

 

DC FieldValueLanguage
dc.contributor.authorLi, YSen_HK
dc.contributor.authorWang, ZXen_HK
dc.contributor.authorLi, Cen_HK
dc.contributor.authorXu, Men_HK
dc.contributor.authorLi, Yen_HK
dc.contributor.authorHuang, WQen_HK
dc.contributor.authorXia, Zen_HK
dc.contributor.authorLiu, KXen_HK
dc.date.accessioned2010-10-31T10:35:55Z-
dc.date.available2010-10-31T10:35:55Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Surgical Research, 2010, v. 164 n. 1, p. e173-e180en_HK
dc.identifier.issn0022-4804en_HK
dc.identifier.urihttp://hdl.handle.net/10722/124466-
dc.description.abstractBackground: Intestinal ischemia/reperfusion (I/R) injury is a critical condition associated with high morbidity and mortality. Our previous study showed that ischemic postconditioning (IPo) protects the intestinal mucosa from I/R injury. However, the precise molecular mechanisms of this event remain poorly elucidated. The aim of this study was to investigate the differentially expressed proteins of intestinal mucosa after intestinal I/R with or without IPo, and to explore the potential mechanisms of intestinal I/R injury and the protective effect of IPo in relation to the differential proteins. Materials and Methods: Intestinal I/R injury was established by occluding the superior mesenteric artery (SMA) for 60min followed by 60min reperfusion. The rats were randomly allocated into one of three groups based upon the intervention (n = 8); sham : sham surgical preparation including isolation of the SMA without occlusion was performed; injury: there was no intervention either before or after SMA occlusion; IPo: three cycles of 30 s reperfusion-30 s reocclusion were imposed immediately upon reperfusion. A comparative proteomics approach with two-dimensional gel electrophoresis was used to isolate proteins in intestinal mucosa, the expression of which were regulated by I/R injury post-treated with or without IPo. The differentially displayed proteins were identified through matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). Results: Image analysis revealed that an average of 1300 protein spots were detected on each gel; 16 and 9 proteins showing more than 1.5-fold difference were identified between the Sham versus Injury group and injury group versus IPo group, respectively. The identified proteins were functionally involved in the cellular processes of energy metabolism, anti-oxidation, and anti-apoptosis. Conclusions: This study provided new clues for understanding the mechanisms of IPo against intestinal I/R injury. © 2010 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc.. The Journal's web site is located at http://www.elsevier.com/locate/jsreen_HK
dc.relation.ispartofJournal of Surgical Researchen_HK
dc.subjectintestineen_HK
dc.subjectischemia/reperfusion injuryen_HK
dc.subjectischemic postconditioningen_HK
dc.subjectproteomicen_HK
dc.subject.meshAnimals-
dc.subject.meshIntestinal Mucosa - blood supply - metabolism - pathology-
dc.subject.meshIschemic Preconditioning - methods-
dc.subject.meshProteomics-
dc.subject.meshReperfusion Injury - metabolism - pathology-
dc.titleProteomics of ischemia/reperfusion injury in rat intestine with and without ischemic postconditioningen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-4804&volume=164&issue=1&spage=e173&epage=180&date=2010&atitle=Proteomics+of+Ischemia/Reperfusion+Injury+in+Rat+Intestine+With+and+Without+Ischemic+Postconditioningen_HK
dc.identifier.emailXia, Z:zyxia@hkucc.hku.hken_HK
dc.identifier.authorityXia, Z=rp00532en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jss.2009.10.003en_HK
dc.identifier.pmid20189595-
dc.identifier.scopuseid_2-s2.0-77958089516en_HK
dc.identifier.hkuros174751en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77958089516&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume164en_HK
dc.identifier.issue1en_HK
dc.identifier.spagee173en_HK
dc.identifier.epagee180en_HK
dc.identifier.isiWOS:000285747600024-
dc.publisher.placeUnited Statesen_HK
dc.identifier.citeulike6046253-

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