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Article: Antifungal Activity of Baicalein Against Candida krusei Does Not Involve Apoptosis

TitleAntifungal Activity of Baicalein Against Candida krusei Does Not Involve Apoptosis
Authors
KeywordsBaicalein
Candidakrusei
Mitochondrial membrane potential
Reactive oxygen species
Issue Date2010
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0301-486X
Citation
Mycopathologia, 2010, v. 170 n. 6, p. 391-396 How to Cite?
AbstractThe present study was designed to evaluate the antifungal activity of baicalein against Candida krusei isolates. Using a broth microdilution assay, baicalein exhibited potent in vitro antifungal activity against C. krusei isolates with a minimum inhibitory concentration of 2. 7 μg/ml. Flow cytometric study indicated that baicalein depolarized mitochondrial membrane potential in a concentration-dependent manner. However, mechanistic analyses showed that the intracellular reactive oxygen species (ROS) level was virtually unchanged, and massive DNA fragmentation was not observed in C. krusei isolates after baicalein treatment even at a concentration which was apoptotic in C. albicans. Taken together, we conclude that the antifungal activity of baicalein in C. krusei isolates occurs through perturbation in mitochondrial homeostasis without causing elevation of the intracellular ROS level and does not involve apoptosis. © 2010 Springer Science+Business Media B.V.
Persistent Identifierhttp://hdl.handle.net/10722/124457
ISSN
2015 Impact Factor: 1.671
2015 SCImago Journal Rankings: 0.720
ISI Accession Number ID
Funding AgencyGrant Number
Research Fund for the Control of Infectious DiseasesRFCID 08070142
Food and Health Bureau of the Government of the Hong Kong SAR
Funding Information:

This research was supported by a research grant to PWK Tsang from the Research Fund for the Control of Infectious Diseases (RFCID 08070142), the Food and Health Bureau of the Government of the Hong Kong SAR. We thank Prof. L. P. Samaranayake (The University of Hong Kong) for providing C. krusei CK25 and Dr. J. Morschhauser (The University of Wurzburg) for C. albicans SC5314 used in this study.

References

 

DC FieldValueLanguage
dc.contributor.authorKang, Ken_HK
dc.contributor.authorFong, WPen_HK
dc.contributor.authorTsang, PWKen_HK
dc.date.accessioned2010-10-31T10:35:23Z-
dc.date.available2010-10-31T10:35:23Z-
dc.date.issued2010en_HK
dc.identifier.citationMycopathologia, 2010, v. 170 n. 6, p. 391-396en_HK
dc.identifier.issn0301-486Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/124457-
dc.description.abstractThe present study was designed to evaluate the antifungal activity of baicalein against Candida krusei isolates. Using a broth microdilution assay, baicalein exhibited potent in vitro antifungal activity against C. krusei isolates with a minimum inhibitory concentration of 2. 7 μg/ml. Flow cytometric study indicated that baicalein depolarized mitochondrial membrane potential in a concentration-dependent manner. However, mechanistic analyses showed that the intracellular reactive oxygen species (ROS) level was virtually unchanged, and massive DNA fragmentation was not observed in C. krusei isolates after baicalein treatment even at a concentration which was apoptotic in C. albicans. Taken together, we conclude that the antifungal activity of baicalein in C. krusei isolates occurs through perturbation in mitochondrial homeostasis without causing elevation of the intracellular ROS level and does not involve apoptosis. © 2010 Springer Science+Business Media B.V.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0301-486Xen_HK
dc.relation.ispartofMycopathologiaen_HK
dc.rightsThe original publication is available at www.springerlink.com-
dc.subjectBaicaleinen_HK
dc.subjectCandidakruseien_HK
dc.subjectMitochondrial membrane potentialen_HK
dc.subjectReactive oxygen speciesen_HK
dc.titleAntifungal Activity of Baicalein Against Candida krusei Does Not Involve Apoptosisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0301-486X&volume=170&issue=6&spage=391&epage=396&date=2010&atitle=Antifungal+activity+of+Baicalein+against+Candida+krusei+does+not+involve+apoptosisen_HK
dc.identifier.emailTsang, PWK:pwktsang@hku.hken_HK
dc.identifier.authorityTsang, PWK=rp01388en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s11046-010-9341-2en_HK
dc.identifier.pmid20614252-
dc.identifier.scopuseid_2-s2.0-78049344946en_HK
dc.identifier.hkuros175334en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78049344946&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume170en_HK
dc.identifier.issue6en_HK
dc.identifier.spage391en_HK
dc.identifier.epage396en_HK
dc.identifier.isiWOS:000283510800003-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridKang, K=36544735400en_HK
dc.identifier.scopusauthoridFong, WP=7102816006en_HK
dc.identifier.scopusauthoridTsang, PWK=8334953500en_HK
dc.identifier.citeulike7499954-

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