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Article: Comparing two quantitative methods for studying remineralization of artificial caries

TitleComparing two quantitative methods for studying remineralization of artificial caries
Authors
KeywordsDental caries
MicroCT
Polarized light microscopy
Remineralization
Transverse microradiography
Issue Date2010
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdent
Citation
Journal Of Dentistry, 2010, v. 38 n. 4, p. 352-359 How to Cite?
AbstractObjectives: To compare the detection of changes before and after remineralization of artificial enamel and dentin caries by microCT scanning, polarized light microscopy (PLM) and transverse microradiography (TMR). Methods: Fourteen extracted premolars were cut into tooth blocks and painted with an acid-resistant varnish leaving one enamel and one dentin surface exposed. The tooth blocks were immersed into demineralizing solution for 4 days to produce artificial caries-like lesions and scanned by microCT. Then the 14 tooth blocks were randomly allocated into two groups. Seven tooth blocks in Group I were cut longitudinally through the exposed surface into 100-150 μm thick sections and microradiographs were taken. The other seven tooth blocks in Group II were left intact. All the tooth blocks and sections were then immersed into remineralizing solution for 5 days. PLM and TMR of the tooth sections in Group I were taken again. Depth of the lesion on the TMR was measured. Tooth blocks in Group II were scanned by microCT. Results: Mean lesion depth in Group I reduced by 13.0% and 8.2% after remineralization for enamel and dentin, respectively (paired t-test, P < 0.001). In Group II, linear attenuation coefficient (LAC) of the region of interest (ROI) increased by 11.1% and 23.8% after remineralization for enamel and dentin lesions, respectively (paired t-test, P < 0.001). Conclusion: Both microCT and microradiography are able to detect a change of similar magnitude in the artificial caries lesions after remineralization. MicroCT may be used to substitute TMR and PLM in in vitro studies about caries. © 2010 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/124440
ISSN
2015 Impact Factor: 3.109
2015 SCImago Journal Rankings: 1.029
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLo, ECMen_HK
dc.contributor.authorZhi, QHen_HK
dc.contributor.authorItthagarun, Aen_HK
dc.date.accessioned2010-10-31T10:34:29Z-
dc.date.available2010-10-31T10:34:29Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Dentistry, 2010, v. 38 n. 4, p. 352-359en_HK
dc.identifier.issn0300-5712en_HK
dc.identifier.urihttp://hdl.handle.net/10722/124440-
dc.description.abstractObjectives: To compare the detection of changes before and after remineralization of artificial enamel and dentin caries by microCT scanning, polarized light microscopy (PLM) and transverse microradiography (TMR). Methods: Fourteen extracted premolars were cut into tooth blocks and painted with an acid-resistant varnish leaving one enamel and one dentin surface exposed. The tooth blocks were immersed into demineralizing solution for 4 days to produce artificial caries-like lesions and scanned by microCT. Then the 14 tooth blocks were randomly allocated into two groups. Seven tooth blocks in Group I were cut longitudinally through the exposed surface into 100-150 μm thick sections and microradiographs were taken. The other seven tooth blocks in Group II were left intact. All the tooth blocks and sections were then immersed into remineralizing solution for 5 days. PLM and TMR of the tooth sections in Group I were taken again. Depth of the lesion on the TMR was measured. Tooth blocks in Group II were scanned by microCT. Results: Mean lesion depth in Group I reduced by 13.0% and 8.2% after remineralization for enamel and dentin, respectively (paired t-test, P < 0.001). In Group II, linear attenuation coefficient (LAC) of the region of interest (ROI) increased by 11.1% and 23.8% after remineralization for enamel and dentin lesions, respectively (paired t-test, P < 0.001). Conclusion: Both microCT and microradiography are able to detect a change of similar magnitude in the artificial caries lesions after remineralization. MicroCT may be used to substitute TMR and PLM in in vitro studies about caries. © 2010 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdenten_HK
dc.relation.ispartofJournal of Dentistryen_HK
dc.subjectDental cariesen_HK
dc.subjectMicroCTen_HK
dc.subjectPolarized light microscopyen_HK
dc.subjectRemineralizationen_HK
dc.subjectTransverse microradiographyen_HK
dc.subject.meshDental Caries - pathology - therapy-
dc.subject.meshDental Enamel - pathology-
dc.subject.meshDentin - pathology-
dc.subject.meshDiagnostic Imaging - methods-
dc.subject.meshTooth Remineralization - methods-
dc.titleComparing two quantitative methods for studying remineralization of artificial cariesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0300-5712&volume=38&issue=4&spage=352&epage=359&date=2010&atitle=Comparing+two+quantitative+methods+for+studying+remineralization+of+artificial+cariesen_HK
dc.identifier.emailLo, ECM:hrdplcm@hkucc.hku.hken_HK
dc.identifier.authorityLo, ECM=rp00015en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jdent.2010.01.001en_HK
dc.identifier.pmid20079396-
dc.identifier.scopuseid_2-s2.0-77649191102en_HK
dc.identifier.hkuros174422en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77649191102&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume38en_HK
dc.identifier.issue4en_HK
dc.identifier.spage352en_HK
dc.identifier.epage359en_HK
dc.identifier.eissn1879-176X-
dc.identifier.isiWOS:000276118500012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLo, ECM=7101705982en_HK
dc.identifier.scopusauthoridZhi, QH=35363234800en_HK
dc.identifier.scopusauthoridItthagarun, A=6701591745en_HK
dc.identifier.citeulike6554404-

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