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Article: Male germ cell-specific protein Trs4 binds to multiple proteins

TitleMale germ cell-specific protein Trs4 binds to multiple proteins
Authors
KeywordsCryptorchidism
IQ calmodulin-binding motif
Spermatogenesis
Testis
Trs4
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2009, v. 388 n. 3, p. 583-588 How to Cite?
AbstractTemperature-related sequence 4 (Trs4) has been identified as a testis-specific gene with expression sensitive to the abdominal temperature changes induced by artificial cryptorchidism. In murine testes, Trs4 mRNA was detected in round spermatids and its protein was localized mainly in the elongating spermatids as well as in the acrosomes and tails of mature spermatozoa. Using a yeast two-hybrid screening system, we identified Rshl-2, Gstmu1, and Ddc8 as putative binding partners of the Trs4 protein in mouse testes. Their interactions were confirmed by in vivo and in vitro binding assays. Further studies demonstrated that Ddc8, a newly identified gene with unknown functions, displayed a similar expression pattern with Trs4 in mouse testes. In particular, Trs4, Ddc8, and Rshl-2 proteins were co-localized to the tails of mature spermatozoa. These results suggested that Trs4 might be involved in diverse processes of spermiogenesis and/or fertilization through interactions with its multiple binding partners. © 2009 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/124158
ISSN
2014 Impact Factor: 2.297
ISI Accession Number ID
Funding AgencyGrant Number
National Basic Research Program of China2006CB944000
2006CB944004
National "973" Program2006CB504001
NSFC-RGC30618005
N_HKU712/06
Beijing NSF5073032
CAS Knowledge Innovation ProjectKSCX2-YW-R-5
National Natural Science Foundation of China90508008
Funding Information:

This study was supported by the National Basic Research Program of China (2006CB944000, 2006CB944004), the National "973" Program (2006CB504001), the NSFC-RGC joint Project (30618005 and N_HKU712/06), Beijing NSF (5073032), the CAS Knowledge Innovation Project (KSCX2-YW-R-5) and the National Natural Science Foundation of China (90508008).

References

 

DC FieldValueLanguage
dc.contributor.authorShi, YQen_HK
dc.contributor.authorLi, YCen_HK
dc.contributor.authorHu, XQen_HK
dc.contributor.authorLiu, Ten_HK
dc.contributor.authorLiao, SYen_HK
dc.contributor.authorGuo, Jen_HK
dc.contributor.authorHuang, Len_HK
dc.contributor.authorHu, ZYen_HK
dc.contributor.authorTang, AYBen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorHan, CSen_HK
dc.contributor.authorLiu, YXen_HK
dc.date.accessioned2010-10-28T06:50:52Z-
dc.date.available2010-10-28T06:50:52Z-
dc.date.issued2009en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2009, v. 388 n. 3, p. 583-588en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/124158-
dc.description.abstractTemperature-related sequence 4 (Trs4) has been identified as a testis-specific gene with expression sensitive to the abdominal temperature changes induced by artificial cryptorchidism. In murine testes, Trs4 mRNA was detected in round spermatids and its protein was localized mainly in the elongating spermatids as well as in the acrosomes and tails of mature spermatozoa. Using a yeast two-hybrid screening system, we identified Rshl-2, Gstmu1, and Ddc8 as putative binding partners of the Trs4 protein in mouse testes. Their interactions were confirmed by in vivo and in vitro binding assays. Further studies demonstrated that Ddc8, a newly identified gene with unknown functions, displayed a similar expression pattern with Trs4 in mouse testes. In particular, Trs4, Ddc8, and Rshl-2 proteins were co-localized to the tails of mature spermatozoa. These results suggested that Trs4 might be involved in diverse processes of spermiogenesis and/or fertilization through interactions with its multiple binding partners. © 2009 Elsevier Inc.en_HK
dc.languageeng-
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectCryptorchidismen_HK
dc.subjectIQ calmodulin-binding motifen_HK
dc.subjectSpermatogenesisen_HK
dc.subjectTestisen_HK
dc.subjectTrs4en_HK
dc.subject.meshAmino Acid Sequence-
dc.subject.meshCarrier Proteins - genetics - metabolism-
dc.subject.meshSpermatogenesis-
dc.subject.meshSpermatozoa - metabolism-
dc.subject.meshTestis - metabolism-
dc.titleMale germ cell-specific protein Trs4 binds to multiple proteinsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=388&spage=583&epage=588&date=2009&atitle=Male+germ+cell-specific+protein+Trs4+binds+to+multiple+proteins-
dc.identifier.emailLee, KF:ckflee@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.bbrc.2009.08.053en_HK
dc.identifier.pmid19706271en_HK
dc.identifier.scopuseid_2-s2.0-69549138575en_HK
dc.identifier.hkuros173151-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-69549138575&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume388en_HK
dc.identifier.issue3en_HK
dc.identifier.spage583en_HK
dc.identifier.epage588en_HK
dc.identifier.isiWOS:000275061900022-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridShi, YQ=12344557000en_HK
dc.identifier.scopusauthoridLi, YC=35285987900en_HK
dc.identifier.scopusauthoridHu, XQ=24399019800en_HK
dc.identifier.scopusauthoridLiu, T=26661342300en_HK
dc.identifier.scopusauthoridLiao, SY=16744845900en_HK
dc.identifier.scopusauthoridGuo, J=8636694800en_HK
dc.identifier.scopusauthoridHuang, L=35210000200en_HK
dc.identifier.scopusauthoridHu, ZY=7404211495en_HK
dc.identifier.scopusauthoridTang, AYB=40262704600en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridHan, CS=7403379501en_HK
dc.identifier.scopusauthoridLiu, YX=36063638000en_HK
dc.identifier.citeulike5442614-

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