File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Phylogenetic diversity and axial distribution of microbes in the intestinal tract of the polychaete Neanthes glandicincta

TitlePhylogenetic diversity and axial distribution of microbes in the intestinal tract of the polychaete Neanthes glandicincta
Authors
Issue Date2009
PublisherSpringer New York LLC. The Journal's web site is located at http://link.springer.de/link/service/journals/00248/index.htm
Citation
Microbial Ecology, 2009, v. 58 n. 4, p. 892-902 How to Cite?
AbstractThe phylogenetic diversity and axial distribution of microorganisms in three sections of the gastrointestinal tracts of the polychaete Neanthes glandicincta was evaluated using both most probable number method and cloning analyses of 16S rRNA genes in this study. Quantification of the density of microorganisms in the gut showed that aerobic microorganisms decreased from anterior to posterior, while anaerobic ones showed a reverse trend. The total numbers of microorganisms decreased significantly (p<0.05, analysis of variance) but more rapidly from the anterior to the middle segment. Phylogenetic analysis showed that the dominating phylogenetic groups included Methanomicrobiales I: Methanosaetaceae (up to 66% of archaeal clones), δ-Proteobacteria (up to 42% of bacterial clones), and γ-Proteobacteria (up to 30% of bacterial clones) widely distributed throughout the entire gut. Other microbiota distributed in different gut sections were Methanomicrobiales II: Methanospirillaceae, Methanomicrobiales III, Thermoplasmatales, Crenarchaea, Methanobacteriaceae, and Methanosarcinales for archaea; and α-Proteobacteria, β-Proteobacteria, Fusobacteria, Clostridia, Chloroflexi, and Planctomycetes for bacteria. The results reveal a difference in microbial community structure along the gut of N. glandicincta. The various phylogenetic diversity and axial distribution of microbes along the gut might indicate an environmental gradient from anterior to posterior sections affecting the structure of the microbial community. © Springer Science + Business Media, LLC 2009.
Persistent Identifierhttp://hdl.handle.net/10722/124075
ISSN
2015 Impact Factor: 3.232
2015 SCImago Journal Rankings: 1.346
ISI Accession Number ID
Funding AgencyGrant Number
Research Grant Council of Hong Kong GovernmentCA04/05.SC01
NSFC30570034
Funding Information:

We would like to express our sincere thanks to Dr. Zhenye Zhao and Ms. Jessie Lai at The University of Hong Kong for providing samples and technical assistance in the field. We also like to thank Adam Mumford at The State University of New Jersey for his kind review and comments on this manuscript. This work was supported in part by a research grant CA04/05.SC01 from Research Grant Council of Hong Kong Government (J-DG) and project no. 30570034 from NSFC (HY).

References

 

DC FieldValueLanguage
dc.contributor.authorLi, Men_HK
dc.contributor.authorYang, Hen_HK
dc.contributor.authorGu, JDen_HK
dc.date.accessioned2010-10-20T04:49:34Z-
dc.date.available2010-10-20T04:49:34Z-
dc.date.issued2009en_HK
dc.identifier.citationMicrobial Ecology, 2009, v. 58 n. 4, p. 892-902en_HK
dc.identifier.issn0095-3628en_HK
dc.identifier.urihttp://hdl.handle.net/10722/124075-
dc.description.abstractThe phylogenetic diversity and axial distribution of microorganisms in three sections of the gastrointestinal tracts of the polychaete Neanthes glandicincta was evaluated using both most probable number method and cloning analyses of 16S rRNA genes in this study. Quantification of the density of microorganisms in the gut showed that aerobic microorganisms decreased from anterior to posterior, while anaerobic ones showed a reverse trend. The total numbers of microorganisms decreased significantly (p<0.05, analysis of variance) but more rapidly from the anterior to the middle segment. Phylogenetic analysis showed that the dominating phylogenetic groups included Methanomicrobiales I: Methanosaetaceae (up to 66% of archaeal clones), δ-Proteobacteria (up to 42% of bacterial clones), and γ-Proteobacteria (up to 30% of bacterial clones) widely distributed throughout the entire gut. Other microbiota distributed in different gut sections were Methanomicrobiales II: Methanospirillaceae, Methanomicrobiales III, Thermoplasmatales, Crenarchaea, Methanobacteriaceae, and Methanosarcinales for archaea; and α-Proteobacteria, β-Proteobacteria, Fusobacteria, Clostridia, Chloroflexi, and Planctomycetes for bacteria. The results reveal a difference in microbial community structure along the gut of N. glandicincta. The various phylogenetic diversity and axial distribution of microbes along the gut might indicate an environmental gradient from anterior to posterior sections affecting the structure of the microbial community. © Springer Science + Business Media, LLC 2009.en_HK
dc.languageeng-
dc.publisherSpringer New York LLC. The Journal's web site is located at http://link.springer.de/link/service/journals/00248/index.htmen_HK
dc.relation.ispartofMicrobial Ecologyen_HK
dc.rightsThe original publication is available at www.springerlink.com-
dc.subject.meshAnimals-
dc.subject.meshArchaea - classification - genetics - isolation and purification-
dc.subject.meshBacteria - classification - genetics - isolation and purification-
dc.subject.meshPhylogeny-
dc.subject.meshPolychaeta - microbiology-
dc.titlePhylogenetic diversity and axial distribution of microbes in the intestinal tract of the polychaete Neanthes glandicinctaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-3628&volume=58&issue=4&spage=892&epage=902&date=2009&atitle=Phylogenetic+diversity+and+axial+distribution+of+microbes+in+the+intestinal+tract+of+the+polychaete+Neanthes+glandicincta-
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_HK
dc.identifier.authorityGu, JD=rp00701en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00248-009-9550-8en_HK
dc.identifier.pmid19572164-
dc.identifier.scopuseid_2-s2.0-73349088760en_HK
dc.identifier.hkuros172622-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-73349088760&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume58en_HK
dc.identifier.issue4en_HK
dc.identifier.spage892en_HK
dc.identifier.epage902en_HK
dc.identifier.isiWOS:000271461000018-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLi, M=25628417400en_HK
dc.identifier.scopusauthoridYang, H=7406561423en_HK
dc.identifier.scopusauthoridGu, JD=7403129601en_HK
dc.identifier.citeulike5110925-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats