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Article: Effect of photon flux densities on regulation of carotenogenesis and cell viability of Haematococcus pluvialis (Chlorophyceae)

TitleEffect of photon flux densities on regulation of carotenogenesis and cell viability of Haematococcus pluvialis (Chlorophyceae)
Authors
KeywordsAstaxanthin
Haematococcus pluvialis
High light
mRNA expression
Oxidative stress
Issue Date2010
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0921-8971
Citation
Journal Of Applied Phycology, 2010, v. 22 n. 3, p. 253-263 How to Cite?
AbstractThe green alga Haematococcus pluvialis produces large amounts of the pink carotenoid astaxanthin under high photon flux density (PFD) and other oxidative stress conditions. However, the regulation and physiological role of carotenogenesis leading to astaxanthin formation is not well understood. Comparative transcriptional expression of five carotenoid genes along with growth and pigment composition as a function of PFD was studied using a wild-type and an astaxanthin-overproduction mutant of H. pluvialis NIES144. The results indicate that astaxanthin biosynthesis was mainly under transcriptional control of the gene encoding carotenoid hydroxylase, and to a lesser extent, the genes encoding isopentenyl isomerase and phytoene desaturase, and to the least extent, the genes encoding phytoene synthase and carotenoid oxygenase. The expression of a plastid terminal oxidase (PTOX) gene ptox2 underwent transient up-regulation under elevated PFDs, suggesting that PTOX may be functionally coupled with phytoene desaturase through the plastoquinone pool and may play a role in reducing redox-potential-dependent and oxygen-concentration-dependent formation of reactive oxygen species in the chloroplast. Over-expression of both the carotenogenic and PTOX genes confers to the astaxanthin-overproduction mutant more effective photoprotective capability than that of the wild type under photooxidative stress. © Springer Science+Business Media B.V. 2009.
Persistent Identifierhttp://hdl.handle.net/10722/124061
ISSN
2015 Impact Factor: 2.372
2015 SCImago Journal Rankings: 0.852
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Science Foundation Arizona Small Business Catalytic Program
Research Grants Council of Hong Kong
University of Hong Kong
Funding Information:

This work was partially supported by Science Foundation Arizona Small Business Catalytic Program, the Research Grants Council of Hong Kong, and the University of Hong Kong Outstanding Young Researcher Award and Outstanding Research Student Supervisor Award.

References

 

DC FieldValueLanguage
dc.contributor.authorLi, Yen_HK
dc.contributor.authorSommerfeld, Men_HK
dc.contributor.authorChen, Fen_HK
dc.contributor.authorHu, Qen_HK
dc.date.accessioned2010-10-19T04:37:06Z-
dc.date.available2010-10-19T04:37:06Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Applied Phycology, 2010, v. 22 n. 3, p. 253-263en_HK
dc.identifier.issn0921-8971en_HK
dc.identifier.urihttp://hdl.handle.net/10722/124061-
dc.description.abstractThe green alga Haematococcus pluvialis produces large amounts of the pink carotenoid astaxanthin under high photon flux density (PFD) and other oxidative stress conditions. However, the regulation and physiological role of carotenogenesis leading to astaxanthin formation is not well understood. Comparative transcriptional expression of five carotenoid genes along with growth and pigment composition as a function of PFD was studied using a wild-type and an astaxanthin-overproduction mutant of H. pluvialis NIES144. The results indicate that astaxanthin biosynthesis was mainly under transcriptional control of the gene encoding carotenoid hydroxylase, and to a lesser extent, the genes encoding isopentenyl isomerase and phytoene desaturase, and to the least extent, the genes encoding phytoene synthase and carotenoid oxygenase. The expression of a plastid terminal oxidase (PTOX) gene ptox2 underwent transient up-regulation under elevated PFDs, suggesting that PTOX may be functionally coupled with phytoene desaturase through the plastoquinone pool and may play a role in reducing redox-potential-dependent and oxygen-concentration-dependent formation of reactive oxygen species in the chloroplast. Over-expression of both the carotenogenic and PTOX genes confers to the astaxanthin-overproduction mutant more effective photoprotective capability than that of the wild type under photooxidative stress. © Springer Science+Business Media B.V. 2009.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0921-8971en_HK
dc.relation.ispartofJournal of Applied Phycologyen_HK
dc.rightsSpringer Science+Business Media B.V.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectAstaxanthinen_HK
dc.subjectHaematococcus pluvialisen_HK
dc.subjectHigh lighten_HK
dc.subjectmRNA expressionen_HK
dc.subjectOxidative stressen_HK
dc.titleEffect of photon flux densities on regulation of carotenogenesis and cell viability of Haematococcus pluvialis (Chlorophyceae)en_HK
dc.typeArticleen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1007/s10811-009-9453-6en_HK
dc.identifier.pmid20949119-
dc.identifier.pmcidPMC2946551-
dc.identifier.scopuseid_2-s2.0-77951978729en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77951978729&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.issue3en_HK
dc.identifier.spage253en_HK
dc.identifier.epage263en_HK
dc.identifier.eissn1573-5176en_HK
dc.identifier.isiWOS:000277177900004-
dc.publisher.placeNetherlandsen_HK
dc.description.otherSpringer Open Choice, 01 Dec 2010-
dc.identifier.scopusauthoridLi, Y=8875807300en_HK
dc.identifier.scopusauthoridSommerfeld, M=7007025132en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.scopusauthoridHu, Q=26666082400en_HK
dc.identifier.citeulike4833876-

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