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Article: Microbial community analysis of fresh and old microbial biofilms on Bayon Temple sandstone of Angkor Thom, Cambodia

TitleMicrobial community analysis of fresh and old microbial biofilms on Bayon Temple sandstone of Angkor Thom, Cambodia
Authors
Issue Date2010
PublisherSpringer New York LLC. The Journal's web site is located at http://link.springer.de/link/service/journals/00248/index.htm
Citation
Microbial Ecology, 2010, v. 60 n. 1, p. 105-115 How to Cite?
AbstractThe temples of Angkor monuments including Angkor Thom and Bayon in Cambodia and surrounding countries were exclusively constructed using sandstone. They are severely threatened by biodeterioration caused by active growth of different microorganisms on the sandstone surfaces, but knowledge on the microbial community and composition of the biofilms on the sandstone is not available from this region. This study investigated the microbial community diversity by examining the fresh and old biofilms of the biodeteriorated bas-relief wall surfaces of the Bayon Temple by analysis of 16S and 18S rRNA gene sequences. The results showed that the retrieved sequences were clustered in 11 bacterial, 11 eukaryotic and two archaeal divisions with disparate communities (Acidobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Proteobacteria; Alveolata, Fungi, Metazoa, Viridiplantae; Crenarchaeote, and Euyarchaeota). A comparison of the microbial communities between the fresh and old biofilms revealed that the bacterial community of old biofilm was very similar to the newly formed fresh biofilm in terms of bacterial composition, but the eukaryotic communities were distinctly different between these two. This information has important implications for understanding the formation process and development of the microbial diversity on the sandstone surfaces, and furthermore to the relationship between the extent of biodeterioration and succession of microbial communities on sandstone in tropic region. © 2010 The Author(s).
Persistent Identifierhttp://hdl.handle.net/10722/124060
ISSN
2015 Impact Factor: 3.232
2015 SCImago Journal Rankings: 1.346
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Safeguarding of Bayon Temple of Angkor Thom (JASA, Japan)
APSARA Authority of Cambodian Government
UNESCO/Japanese Funds-in-Trust for the Preservation of the World Cultural Heritage
Funding Information:

We would like to thank the assistance of Xianshu Li and Asako Kusumi in sampling on site. Support of this research project was provided by Safeguarding of Bayon Temple of Angkor Thom (JASA, Japan), APSARA Authority of Cambodian Government, and the UNESCO/Japanese Funds-in-Trust for the Preservation of the World Cultural Heritage.

References

 

DC FieldValueLanguage
dc.contributor.authorLan, Wen_HK
dc.contributor.authorLi, Hen_HK
dc.contributor.authorWang, WDen_HK
dc.contributor.authorKatayama, Yen_HK
dc.contributor.authorGu, JDen_HK
dc.date.accessioned2010-10-19T04:37:02Z-
dc.date.available2010-10-19T04:37:02Z-
dc.date.issued2010en_HK
dc.identifier.citationMicrobial Ecology, 2010, v. 60 n. 1, p. 105-115en_HK
dc.identifier.issn0095-3628en_HK
dc.identifier.urihttp://hdl.handle.net/10722/124060-
dc.description.abstractThe temples of Angkor monuments including Angkor Thom and Bayon in Cambodia and surrounding countries were exclusively constructed using sandstone. They are severely threatened by biodeterioration caused by active growth of different microorganisms on the sandstone surfaces, but knowledge on the microbial community and composition of the biofilms on the sandstone is not available from this region. This study investigated the microbial community diversity by examining the fresh and old biofilms of the biodeteriorated bas-relief wall surfaces of the Bayon Temple by analysis of 16S and 18S rRNA gene sequences. The results showed that the retrieved sequences were clustered in 11 bacterial, 11 eukaryotic and two archaeal divisions with disparate communities (Acidobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Proteobacteria; Alveolata, Fungi, Metazoa, Viridiplantae; Crenarchaeote, and Euyarchaeota). A comparison of the microbial communities between the fresh and old biofilms revealed that the bacterial community of old biofilm was very similar to the newly formed fresh biofilm in terms of bacterial composition, but the eukaryotic communities were distinctly different between these two. This information has important implications for understanding the formation process and development of the microbial diversity on the sandstone surfaces, and furthermore to the relationship between the extent of biodeterioration and succession of microbial communities on sandstone in tropic region. © 2010 The Author(s).en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://link.springer.de/link/service/journals/00248/index.htmen_HK
dc.relation.ispartofMicrobial Ecologyen_HK
dc.rightsThe original publication is available at www.springerlink.comen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshArchaea - genetics - isolation and purification-
dc.subject.meshBacteria - genetics - isolation and purification-
dc.subject.meshBiofilms - growth and development-
dc.subject.meshConstruction Materials - microbiology-
dc.subject.meshEnvironmental Microbiology-
dc.titleMicrobial community analysis of fresh and old microbial biofilms on Bayon Temple sandstone of Angkor Thom, Cambodiaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-3628&volume=60&issue=1&spage=105&epage=115&date=2010&atitle=Microbial+community+analysis+of+fresh+and+old+microbial+biofilms+on+Bayon+Temple+sandstone+of+Angkor+Thom,+Cambodia-
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_HK
dc.identifier.authorityGu, JD=rp00701en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1007/s00248-010-9707-5en_HK
dc.identifier.pmid20593173-
dc.identifier.pmcidPMC2917545-
dc.identifier.scopuseid_2-s2.0-77955417687en_HK
dc.identifier.hkuros194830-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77955417687&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume60en_HK
dc.identifier.issue1en_HK
dc.identifier.spage105en_HK
dc.identifier.epage115en_HK
dc.identifier.eissn1432-184Xen_HK
dc.identifier.isiWOS:000280701500009-
dc.publisher.placeUnited Statesen_HK
dc.description.otherSpringer Open Choice, 01 Dec 2010-
dc.identifier.scopusauthoridLan, W=36885898300en_HK
dc.identifier.scopusauthoridLi, H=36077120500en_HK
dc.identifier.scopusauthoridWang, WD=36544658800en_HK
dc.identifier.scopusauthoridKatayama, Y=7402294354en_HK
dc.identifier.scopusauthoridGu, JD=7403129601en_HK
dc.identifier.citeulike7416510-

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