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Article: Generation of human induced pluripotent stem cells from umbilical cord matrix and amniotic membrane mesenchymal cells

TitleGeneration of human induced pluripotent stem cells from umbilical cord matrix and amniotic membrane mesenchymal cells
Authors
Issue Date2010
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 2010, v. 285 n. 15, p. 11227-11234 How to Cite?
AbstractThe umbilical cord and placenta are extra-embryonic tissues of particular interest for regenerative medicine. They share an early developmental origin and are a source of vast amounts of cells with multilineage differentiation potential that are poorly immunogenic and without controversy. Moreover, these cells are likely exempt from incorporated mutations when compared with juvenile or adult donor cells such as skin fibroblasts or keratinocytes. Here we report the efficient generation of induced pluripotent stem cells (iPSCs) from mesenchymal cells of the umbilical cord matrix (up to 0.4% of the cells became reprogrammed) and the placental amniotic membrane (up to 0.1%) using exogenous factors and a chemical mixture. iPSCs from these 2 tissues homogeneously showed human embryonic stem cell (hESC)-like characteristics including morphology, positive staining for alkaline phosphatase, normal karyotype, and expression of hESC-like markers including Nanog, Rex1, Oct4, TRA-1-60, TRA-1-80, SSEA-3, and SSEA-4. Selected clones also formed embryonic bodies and teratomas containing derivatives of the 3 germ layers, and could as well be readily differentiated into functional motor neurons. Among other things, our cell lines may prove useful for comparisons between iPSCs derived from multiple tissues regarding the extent of the epigenetic reprogramming, differentiation ability, stability of the resulting lineages, and the risk of associated abnormalities. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/123990
ISSN
2014 Impact Factor: 4.573
2014 SCImago Journal Rankings: 2.734
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Chinese Academy of SciencesKSCX2-YW-R-48
KSCX2-YW-R-244
National Natural Science Foundation of China30725012
230725012
90813033
30630039
Ministry of Science and Technology 973 Program2006CB701504
2006CB943600
2007CB948002
2007CB947804
2007CB947900
2009CB941102
2009CB940902
2010CB944800
National High Technology Research and Development Program of China2006AA02A103
2007G-P034
2007Z3-C7031
Bureau of Science and Technology of Guangzhou Municipality2006A50104002
2008A1-E4011
EFBIC (European Federation of Biotechnology)
Funding Information:

This work was supported by the Knowledge Innovation Program of the Chinese Academy of Sciences, Chinese Academy of Sciences/SAFEA International Partnership Program for Creative Research Teams, Chinese Academy of Sciences (KSCX2-YW-R-48, KSCX2-YW-R-244), National Natural Science Foundation of China (30725012, 230725012, 90813033, 30630039), Ministry of Science and Technology 973 Program (2006CB701504, 2006CB943600, 2007CB948002, 2007CB947804, 2007CB947900, 2009CB941102, 2009CB940902, 2010CB944800), National High Technology Research and Development Program of China (2006AA02A103, 2007G-P034, 2007Z3-C7031), Bureau of Science and Technology of Guangzhou Municipality (2006A50104002, 2008A1-E4011), and an EFBIC (European Federation of Biotechnology) RED travel grant.

References

 

DC FieldValueLanguage
dc.contributor.authorCai, Jen_HK
dc.contributor.authorLi, Wen_HK
dc.contributor.authorSu, Hen_HK
dc.contributor.authorQin, Den_HK
dc.contributor.authorYang, Jen_HK
dc.contributor.authorZhu, Fen_HK
dc.contributor.authorXu, Jen_HK
dc.contributor.authorHe, Wen_HK
dc.contributor.authorGuo, Xen_HK
dc.contributor.authorLabuda, Ken_HK
dc.contributor.authorPeterbauer, Aen_HK
dc.contributor.authorWolbank, Sen_HK
dc.contributor.authorZhong, Men_HK
dc.contributor.authorLi, Zen_HK
dc.contributor.authorWu, WTen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorRedl, Hen_HK
dc.contributor.authorZeng, Len_HK
dc.contributor.authorEsteban, MAen_HK
dc.contributor.authorPei, Den_HK
dc.date.accessioned2010-10-15T08:05:57Z-
dc.date.available2010-10-15T08:05:57Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Biological Chemistry, 2010, v. 285 n. 15, p. 11227-11234en_HK
dc.identifier.issn0021-9258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/123990-
dc.description.abstractThe umbilical cord and placenta are extra-embryonic tissues of particular interest for regenerative medicine. They share an early developmental origin and are a source of vast amounts of cells with multilineage differentiation potential that are poorly immunogenic and without controversy. Moreover, these cells are likely exempt from incorporated mutations when compared with juvenile or adult donor cells such as skin fibroblasts or keratinocytes. Here we report the efficient generation of induced pluripotent stem cells (iPSCs) from mesenchymal cells of the umbilical cord matrix (up to 0.4% of the cells became reprogrammed) and the placental amniotic membrane (up to 0.1%) using exogenous factors and a chemical mixture. iPSCs from these 2 tissues homogeneously showed human embryonic stem cell (hESC)-like characteristics including morphology, positive staining for alkaline phosphatase, normal karyotype, and expression of hESC-like markers including Nanog, Rex1, Oct4, TRA-1-60, TRA-1-80, SSEA-3, and SSEA-4. Selected clones also formed embryonic bodies and teratomas containing derivatives of the 3 germ layers, and could as well be readily differentiated into functional motor neurons. Among other things, our cell lines may prove useful for comparisons between iPSCs derived from multiple tissues regarding the extent of the epigenetic reprogramming, differentiation ability, stability of the resulting lineages, and the risk of associated abnormalities. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.en_HK
dc.languageeng-
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_HK
dc.relation.ispartofJournal of Biological Chemistryen_HK
dc.subject.meshAmnion - metabolism-
dc.subject.meshCell Culture Techniques - methods-
dc.subject.meshGene Expression Regulation-
dc.subject.meshMesenchymal Stem Cells - cytology - metabolism-
dc.subject.meshPluripotent Stem Cells - cytology - metabolism-
dc.titleGeneration of human induced pluripotent stem cells from umbilical cord matrix and amniotic membrane mesenchymal cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9258&volume=285&issue=15&spage=11227&epage=11234&date=2010&atitle=Generation+of+human+induced+pluripotent+stem+cells+from+umbilical+cord+matrix+and+amniotic+membrane+mesenchymal+cells-
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1074/jbc.M109.086389en_HK
dc.identifier.pmid20139068en_HK
dc.identifier.pmcidPMC2857000-
dc.identifier.scopuseid_2-s2.0-77951242803en_HK
dc.identifier.hkuros170576-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77951242803&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume285en_HK
dc.identifier.issue15en_HK
dc.identifier.spage11227en_HK
dc.identifier.epage11234en_HK
dc.identifier.isiWOS:000276286200028-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCai, J=9246458800en_HK
dc.identifier.scopusauthoridLi, W=36068145000en_HK
dc.identifier.scopusauthoridSu, H=16317750200en_HK
dc.identifier.scopusauthoridQin, D=23005734400en_HK
dc.identifier.scopusauthoridYang, J=14026282100en_HK
dc.identifier.scopusauthoridZhu, F=36464469100en_HK
dc.identifier.scopusauthoridXu, J=16067616200en_HK
dc.identifier.scopusauthoridHe, W=16316181100en_HK
dc.identifier.scopusauthoridGuo, X=36463626600en_HK
dc.identifier.scopusauthoridLabuda, K=35750199200en_HK
dc.identifier.scopusauthoridPeterbauer, A=7801330954en_HK
dc.identifier.scopusauthoridWolbank, S=6508220567en_HK
dc.identifier.scopusauthoridZhong, M=35265387400en_HK
dc.identifier.scopusauthoridLi, Z=15728094300en_HK
dc.identifier.scopusauthoridWu, T=9237315300en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridRedl, H=7101771621en_HK
dc.identifier.scopusauthoridZeng, L=35750989000en_HK
dc.identifier.scopusauthoridEsteban, MA=35591774300en_HK
dc.identifier.scopusauthoridPei, D=7102806599en_HK

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