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Article: Generation of human induced pluripotent stem cells from umbilical cord matrix and amniotic membrane mesenchymal cells
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TitleGeneration of human induced pluripotent stem cells from umbilical cord matrix and amniotic membrane mesenchymal cells
 
AuthorsCai, J3
Li, W3
Su, H1
Qin, D3
Yang, J3
Zhu, F3
Xu, J3
He, W3
Guo, X3
Labuda, K5
Peterbauer, A4
Wolbank, S5
Zhong, M2
Li, Z3
Wu, WT
So, KF1
Redl, H5
Zeng, L3
Esteban, MA3
Pei, D3
 
Issue Date2010
 
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
 
CitationJournal Of Biological Chemistry, 2010, v. 285 n. 15, p. 11227-11234 [How to Cite?]
DOI: http://dx.doi.org/10.1074/jbc.M109.086389
 
AbstractThe umbilical cord and placenta are extra-embryonic tissues of particular interest for regenerative medicine. They share an early developmental origin and are a source of vast amounts of cells with multilineage differentiation potential that are poorly immunogenic and without controversy. Moreover, these cells are likely exempt from incorporated mutations when compared with juvenile or adult donor cells such as skin fibroblasts or keratinocytes. Here we report the efficient generation of induced pluripotent stem cells (iPSCs) from mesenchymal cells of the umbilical cord matrix (up to 0.4% of the cells became reprogrammed) and the placental amniotic membrane (up to 0.1%) using exogenous factors and a chemical mixture. iPSCs from these 2 tissues homogeneously showed human embryonic stem cell (hESC)-like characteristics including morphology, positive staining for alkaline phosphatase, normal karyotype, and expression of hESC-like markers including Nanog, Rex1, Oct4, TRA-1-60, TRA-1-80, SSEA-3, and SSEA-4. Selected clones also formed embryonic bodies and teratomas containing derivatives of the 3 germ layers, and could as well be readily differentiated into functional motor neurons. Among other things, our cell lines may prove useful for comparisons between iPSCs derived from multiple tissues regarding the extent of the epigenetic reprogramming, differentiation ability, stability of the resulting lineages, and the risk of associated abnormalities. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
 
ISSN0021-9258
2012 Impact Factor: 4.651
2012 SCImago Journal Rankings: 2.723
 
DOIhttp://dx.doi.org/10.1074/jbc.M109.086389
 
PubMed Central IDPMC2857000
 
ISI Accession Number IDWOS:000276286200028
Funding AgencyGrant Number
Chinese Academy of SciencesKSCX2-YW-R-48
KSCX2-YW-R-244
National Natural Science Foundation of China30725012
230725012
90813033
30630039
Ministry of Science and Technology 973 Program2006CB701504
2006CB943600
2007CB948002
2007CB947804
2007CB947900
2009CB941102
2009CB940902
2010CB944800
National High Technology Research and Development Program of China2006AA02A103
2007G-P034
2007Z3-C7031
Bureau of Science and Technology of Guangzhou Municipality2006A50104002
2008A1-E4011
EFBIC (European Federation of Biotechnology)
Funding Information:

This work was supported by the Knowledge Innovation Program of the Chinese Academy of Sciences, Chinese Academy of Sciences/SAFEA International Partnership Program for Creative Research Teams, Chinese Academy of Sciences (KSCX2-YW-R-48, KSCX2-YW-R-244), National Natural Science Foundation of China (30725012, 230725012, 90813033, 30630039), Ministry of Science and Technology 973 Program (2006CB701504, 2006CB943600, 2007CB948002, 2007CB947804, 2007CB947900, 2009CB941102, 2009CB940902, 2010CB944800), National High Technology Research and Development Program of China (2006AA02A103, 2007G-P034, 2007Z3-C7031), Bureau of Science and Technology of Guangzhou Municipality (2006A50104002, 2008A1-E4011), and an EFBIC (European Federation of Biotechnology) RED travel grant.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorCai, J
 
dc.contributor.authorLi, W
 
dc.contributor.authorSu, H
 
dc.contributor.authorQin, D
 
dc.contributor.authorYang, J
 
dc.contributor.authorZhu, F
 
dc.contributor.authorXu, J
 
dc.contributor.authorHe, W
 
dc.contributor.authorGuo, X
 
dc.contributor.authorLabuda, K
 
dc.contributor.authorPeterbauer, A
 
dc.contributor.authorWolbank, S
 
dc.contributor.authorZhong, M
 
dc.contributor.authorLi, Z
 
dc.contributor.authorWu, WT
 
dc.contributor.authorSo, KF
 
dc.contributor.authorRedl, H
 
dc.contributor.authorZeng, L
 
dc.contributor.authorEsteban, MA
 
dc.contributor.authorPei, D
 
dc.date.accessioned2010-10-15T08:05:57Z
 
dc.date.available2010-10-15T08:05:57Z
 
dc.date.issued2010
 
dc.description.abstractThe umbilical cord and placenta are extra-embryonic tissues of particular interest for regenerative medicine. They share an early developmental origin and are a source of vast amounts of cells with multilineage differentiation potential that are poorly immunogenic and without controversy. Moreover, these cells are likely exempt from incorporated mutations when compared with juvenile or adult donor cells such as skin fibroblasts or keratinocytes. Here we report the efficient generation of induced pluripotent stem cells (iPSCs) from mesenchymal cells of the umbilical cord matrix (up to 0.4% of the cells became reprogrammed) and the placental amniotic membrane (up to 0.1%) using exogenous factors and a chemical mixture. iPSCs from these 2 tissues homogeneously showed human embryonic stem cell (hESC)-like characteristics including morphology, positive staining for alkaline phosphatase, normal karyotype, and expression of hESC-like markers including Nanog, Rex1, Oct4, TRA-1-60, TRA-1-80, SSEA-3, and SSEA-4. Selected clones also formed embryonic bodies and teratomas containing derivatives of the 3 germ layers, and could as well be readily differentiated into functional motor neurons. Among other things, our cell lines may prove useful for comparisons between iPSCs derived from multiple tissues regarding the extent of the epigenetic reprogramming, differentiation ability, stability of the resulting lineages, and the risk of associated abnormalities. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationJournal Of Biological Chemistry, 2010, v. 285 n. 15, p. 11227-11234 [How to Cite?]
DOI: http://dx.doi.org/10.1074/jbc.M109.086389
 
dc.identifier.doihttp://dx.doi.org/10.1074/jbc.M109.086389
 
dc.identifier.epage11234
 
dc.identifier.hkuros170576
 
dc.identifier.isiWOS:000276286200028
Funding AgencyGrant Number
Chinese Academy of SciencesKSCX2-YW-R-48
KSCX2-YW-R-244
National Natural Science Foundation of China30725012
230725012
90813033
30630039
Ministry of Science and Technology 973 Program2006CB701504
2006CB943600
2007CB948002
2007CB947804
2007CB947900
2009CB941102
2009CB940902
2010CB944800
National High Technology Research and Development Program of China2006AA02A103
2007G-P034
2007Z3-C7031
Bureau of Science and Technology of Guangzhou Municipality2006A50104002
2008A1-E4011
EFBIC (European Federation of Biotechnology)
Funding Information:

This work was supported by the Knowledge Innovation Program of the Chinese Academy of Sciences, Chinese Academy of Sciences/SAFEA International Partnership Program for Creative Research Teams, Chinese Academy of Sciences (KSCX2-YW-R-48, KSCX2-YW-R-244), National Natural Science Foundation of China (30725012, 230725012, 90813033, 30630039), Ministry of Science and Technology 973 Program (2006CB701504, 2006CB943600, 2007CB948002, 2007CB947804, 2007CB947900, 2009CB941102, 2009CB940902, 2010CB944800), National High Technology Research and Development Program of China (2006AA02A103, 2007G-P034, 2007Z3-C7031), Bureau of Science and Technology of Guangzhou Municipality (2006A50104002, 2008A1-E4011), and an EFBIC (European Federation of Biotechnology) RED travel grant.

 
dc.identifier.issn0021-9258
2012 Impact Factor: 4.651
2012 SCImago Journal Rankings: 2.723
 
dc.identifier.issue15
 
dc.identifier.openurl
 
dc.identifier.pmcidPMC2857000
 
dc.identifier.pmid20139068
 
dc.identifier.scopuseid_2-s2.0-77951242803
 
dc.identifier.spage11227
 
dc.identifier.urihttp://hdl.handle.net/10722/123990
 
dc.identifier.volume285
 
dc.languageeng
 
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Biological Chemistry
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAmnion - metabolism
 
dc.subject.meshCell Culture Techniques - methods
 
dc.subject.meshGene Expression Regulation
 
dc.subject.meshMesenchymal Stem Cells - cytology - metabolism
 
dc.subject.meshPluripotent Stem Cells - cytology - metabolism
 
dc.titleGeneration of human induced pluripotent stem cells from umbilical cord matrix and amniotic membrane mesenchymal cells
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. Nanfang Hospital
  3. Chinese Academy of Sciences
  4. Austrian Cluster for Tissue Regeneration
  5. null