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Article: Aflatoxin B1 transfer and metabolism in human placenta

TitleAflatoxin B1 transfer and metabolism in human placenta
Authors
KeywordsAflatoxicol
Dietary carcinogen
Fetal exposure
Human placental perfusion
Placental cytosolic fraction
Issue Date2009
PublisherOxford University Press. The Journal's web site is located at http://toxsci.oxfordjournals.org/
Citation
Toxicological Sciences, 2009, v. 113 n. 1, p. 216-225 How to Cite?
AbstractAflatoxin B1 (AFB1), a common dietary contaminant, is a major risk factor of hepatocellular carcinoma (HCC). Early onset of HCC in some countries in Africa and South-East Asia indicates the importance of early life exposure. Placenta is the primary route for various compounds, both nutrients and toxins, from the mother to the fetal circulation. Furthermore, placenta contains enzymes for xenobiotic metabolism. AFB1, AFB1-metabolites, and AFB1-albumin adducts have been detected in cord blood of babies after maternal exposure during pregnancy. However, the role that the placenta plays in the transfer and metabolism of AFB1 is not clear. In this study, placental transfer and metabolism of AFB1 were investigated in human placental perfusions and in in vitro studies. Eight human placentas were perfused with 0.5 or 5μM AFB1 for 2-4 h. In vitro incubations with placental microsomal and cytosolic proteins from eight additional placentas were also conducted. Our results from placental perfusions provide the first direct evidence of the actual transfer of AFB1 and its metabolism to aflatoxicol (AFL) by human placenta. In vitro incubations with placental cytosolic fraction confirmed the capacity of human placenta to form AFL. AFL was the only metabolite detected in both perfusions and in vitro incubations. Since AFL is less mutagenic, but putatively as carcinogenic as AFB1, the formation of AFL may not protect the fetus from the toxicity of AFB1. © The Author 2009. Published by Oxford University Press on behalf of the Society of Toxicology.
Persistent Identifierhttp://hdl.handle.net/10722/123975
ISSN
2015 Impact Factor: 3.88
2015 SCImago Journal Rankings: 1.686
ISI Accession Number ID
Funding AgencyGrant Number
University of Kuopio, Finland
European Union-project NewGenerisFOOD-CT-2005 016320
Finnish Graduate School of Toxicology and Emil Aaltonen Foundation, Finland
Funding Information:

University of Kuopio, Finland; European Union-project NewGeneris (FOOD-CT-2005 016320); Finnish Graduate School of Toxicology and Emil Aaltonen Foundation, Finland.

References

 

DC FieldValueLanguage
dc.contributor.authorPartanen, HAen_HK
dc.contributor.authorElNezami, HSen_HK
dc.contributor.authorLeppänen, JMen_HK
dc.contributor.authorMyllynen, PKen_HK
dc.contributor.authorWoodhouse, HJen_HK
dc.contributor.authorVähäkangas, KHen_HK
dc.date.accessioned2010-10-14T02:20:02Z-
dc.date.available2010-10-14T02:20:02Z-
dc.date.issued2009en_HK
dc.identifier.citationToxicological Sciences, 2009, v. 113 n. 1, p. 216-225en_HK
dc.identifier.issn1096-6080en_HK
dc.identifier.urihttp://hdl.handle.net/10722/123975-
dc.description.abstractAflatoxin B1 (AFB1), a common dietary contaminant, is a major risk factor of hepatocellular carcinoma (HCC). Early onset of HCC in some countries in Africa and South-East Asia indicates the importance of early life exposure. Placenta is the primary route for various compounds, both nutrients and toxins, from the mother to the fetal circulation. Furthermore, placenta contains enzymes for xenobiotic metabolism. AFB1, AFB1-metabolites, and AFB1-albumin adducts have been detected in cord blood of babies after maternal exposure during pregnancy. However, the role that the placenta plays in the transfer and metabolism of AFB1 is not clear. In this study, placental transfer and metabolism of AFB1 were investigated in human placental perfusions and in in vitro studies. Eight human placentas were perfused with 0.5 or 5μM AFB1 for 2-4 h. In vitro incubations with placental microsomal and cytosolic proteins from eight additional placentas were also conducted. Our results from placental perfusions provide the first direct evidence of the actual transfer of AFB1 and its metabolism to aflatoxicol (AFL) by human placenta. In vitro incubations with placental cytosolic fraction confirmed the capacity of human placenta to form AFL. AFL was the only metabolite detected in both perfusions and in vitro incubations. Since AFL is less mutagenic, but putatively as carcinogenic as AFB1, the formation of AFL may not protect the fetus from the toxicity of AFB1. © The Author 2009. Published by Oxford University Press on behalf of the Society of Toxicology.en_HK
dc.languageeng-
dc.publisherOxford University Press. The Journal's web site is located at http://toxsci.oxfordjournals.org/en_HK
dc.relation.ispartofToxicological Sciencesen_HK
dc.subjectAflatoxicolen_HK
dc.subjectDietary carcinogenen_HK
dc.subjectFetal exposureen_HK
dc.subjectHuman placental perfusionen_HK
dc.subjectPlacental cytosolic fractionen_HK
dc.subject.meshAflatoxin B1 - metabolism-
dc.subject.meshAflatoxins - metabolism-
dc.subject.meshMaternal-Fetal Exchange-
dc.subject.meshMutagens - metabolism-
dc.subject.meshPlacenta - cytology - enzymology - metabolism-
dc.titleAflatoxin B1 transfer and metabolism in human placentaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1096-6080&volume=113&issue=1&spage=216&epage=225&date=2010&atitle=Aflatoxin+B1+transfer+and+metabolism+in+human+placenta-
dc.identifier.emailElNezami, HS: elnezami@hkucc.hku.hken_HK
dc.identifier.authorityElNezami, HS=rp00694en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1093/toxsci/kfp257en_HK
dc.identifier.pmid19875679-
dc.identifier.scopuseid_2-s2.0-75249093115en_HK
dc.identifier.hkuros172283-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-75249093115&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume113en_HK
dc.identifier.issue1en_HK
dc.identifier.spage216en_HK
dc.identifier.epage225en_HK
dc.identifier.isiWOS:000272935700021-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridPartanen, HA=35201638100en_HK
dc.identifier.scopusauthoridElNezami, HS=6603690577en_HK
dc.identifier.scopusauthoridLeppänen, JM=26642968300en_HK
dc.identifier.scopusauthoridMyllynen, PK=7004099488en_HK
dc.identifier.scopusauthoridWoodhouse, HJ=35202583100en_HK
dc.identifier.scopusauthoridVähäkangas, KH=7004284731en_HK

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