File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Novel method of cell-free in vitro synthesis of the human fibroblast growth factor 1 gene

TitleNovel method of cell-free in vitro synthesis of the human fibroblast growth factor 1 gene
Authors
KeywordsMedicine
Biology
Biotechnology
Issue Date2010
PublisherHindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/jbb/index.html
Citation
Journal Of Biomedicine And Biotechnology, 2010, v. 2010 How to Cite?
AbstractRecombinant DNA projects generally involve cell-based gene cloning. However, because template DNA is not always readily available, in vitro chemical synthesis of complete genes from DNA oligonucleotides is becoming the preferred method for cloning. This article describes a new, rapid procedure based on Taq polymerase for the precise assembly of DNA oligonucleotides to yield the complete human fibroblast growth factor 1 (FGF1) gene, which is 468bp long and has a G+C content of 51.5. The new method involved two steps: (1) the design of the DNA oligonucleotides to be assembled and (2) the assembly of multiple oligonucleotides by PCR to generate the whole FGF1 gene. The procedure lasted a total of only 2 days, compared with 2 weeks for the conventional procedure. This method of gene synthesis is expected to facilitate various kinds of complex genetic engineering projects that require rapid gene amplification, such as cell-free whole-DNA library construction, as well as the construction of new genes or genes that contain any mutation, restriction site, or DNA tag. © 2010 Peijun Zuo and A. Bakr M. Rabie.
Persistent Identifierhttp://hdl.handle.net/10722/123937
ISSN
2014 Impact Factor: 3.169
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
SRT, Technical Course Development
Funding Information:

The authors thank Dr. Trevor Lane for editorial assistance during the preparation of the manuscript. This work was supported by SRT funding for Technical Course Development.

References

 

DC FieldValueLanguage
dc.contributor.authorZuo, Pen_HK
dc.contributor.authorRabie, ABMen_HK
dc.date.accessioned2010-10-11T05:45:04Z-
dc.date.available2010-10-11T05:45:04Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Biomedicine And Biotechnology, 2010, v. 2010en_HK
dc.identifier.issn1110-7243en_HK
dc.identifier.urihttp://hdl.handle.net/10722/123937-
dc.description.abstractRecombinant DNA projects generally involve cell-based gene cloning. However, because template DNA is not always readily available, in vitro chemical synthesis of complete genes from DNA oligonucleotides is becoming the preferred method for cloning. This article describes a new, rapid procedure based on Taq polymerase for the precise assembly of DNA oligonucleotides to yield the complete human fibroblast growth factor 1 (FGF1) gene, which is 468bp long and has a G+C content of 51.5. The new method involved two steps: (1) the design of the DNA oligonucleotides to be assembled and (2) the assembly of multiple oligonucleotides by PCR to generate the whole FGF1 gene. The procedure lasted a total of only 2 days, compared with 2 weeks for the conventional procedure. This method of gene synthesis is expected to facilitate various kinds of complex genetic engineering projects that require rapid gene amplification, such as cell-free whole-DNA library construction, as well as the construction of new genes or genes that contain any mutation, restriction site, or DNA tag. © 2010 Peijun Zuo and A. Bakr M. Rabie.en_HK
dc.languageeng-
dc.publisherHindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/jbb/index.htmlen_HK
dc.relation.ispartofJournal of Biomedicine and Biotechnologyen_HK
dc.subjectMedicine-
dc.subjectBiology-
dc.subjectBiotechnology-
dc.titleNovel method of cell-free in vitro synthesis of the human fibroblast growth factor 1 geneen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1110-7243&volume=2010, article no. 971340&spage=&epage=&date=2010&atitle=Novel+method+of+cell-free+in+vitro+synthesis+of+the+human+fibroblast+growth+factor+1+gene-
dc.identifier.emailZuo, P: peijunzuo@lycos.comen_HK
dc.identifier.emailRabie, ABM: rabie@hku.hken_HK
dc.identifier.authorityZuo, P=rp00050en_HK
dc.identifier.authorityRabie, ABM=rp00029en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1155/2010/971340en_HK
dc.identifier.pmid20706664-
dc.identifier.pmcidPMC2913909-
dc.identifier.scopuseid_2-s2.0-77956748732en_HK
dc.identifier.hkuros171981-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77956748732&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume2010en_HK
dc.identifier.isiWOS:000281301700001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZuo, P=36490907500en_HK
dc.identifier.scopusauthoridRabie, ABM=7007172734en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats