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Article: Different mechanisms of cis-9,trans-11- and trans-10,cis-12- conjugated linoleic acid affecting lipid metabolism in 3T3-L1 cells
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TitleDifferent mechanisms of cis-9,trans-11- and trans-10,cis-12- conjugated linoleic acid affecting lipid metabolism in 3T3-L1 cells
 
AuthorsZhai, JJ2 3
Liu, ZL4
Li, JM3
Chen, JP1
Jiang, L3
Wang, DM3
Yuan, J2
Shen, JG1
Yang, DP3
Chen, JQ2
 
Keywords3T3-L1
Conjugated linoleic acid
Lipid metabolism
 
Issue Date2010
 
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/jnutbio
 
CitationJournal Of Nutritional Biochemistry, 2010, v. 21 n. 11, p. 1099-1105 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jnutbio.2009.09.007
 
AbstractConjugated linoleic acid (CLA) has been shown to reduce body fat mass in various experimental animals. It is valuable to identify its influence on enzymes involved in energy expenditure, apoptosis, fatty acid oxidation and lipolysis. We investigated isomer-specific effects of high dose, long treatment of CLA (75.4 Μmol/L, 8 days) on protein and gene expression of these enzymes in cultured 3T3-L1 cells. Proteomics identified significant up- or down-regulation of 52 proteins by either CLA isomer. Protein and gene expression of uncoupling protein (UCP) 1, UCP3, perilipin and peroxisome proliferator-activated receptor (PPAR) α increased whereas UCP2 reduced for both CLA isomers. And eight-day treatment of trans-10,. cis-12 CLA, but not cis-9,. trans-11 CLA, significantly up-regulated protein and mRNA levels of PKA (P<05), CPT-1 and TNF-α (P<01). Compared to protein expression, both isomers did not significantly influence the mRNA expression of HSL, ATGL, ACO and leptin. In conclusion, high-dose, long treatment of cis-9,. trans-11 CLA did not promote apoptosis, fatty acid oxidation and lipolysis in adipocytes, but may induce an increase in energy expenditure. trans-10,. cis-12 CLA exhibited greater influence on lipid metabolism, stimulated adipocyte energy expenditure, apoptosis and fatty acid oxidation, but its effect on lipolysis was not obvious. © 2010 Elsevier Inc.
 
ISSN0955-2863
2013 Impact Factor: 4.592
2013 SCImago Journal Rankings: 1.627
 
DOIhttp://dx.doi.org/10.1016/j.jnutbio.2009.09.007
 
ISI Accession Number IDWOS:000283825600010
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorZhai, JJ
 
dc.contributor.authorLiu, ZL
 
dc.contributor.authorLi, JM
 
dc.contributor.authorChen, JP
 
dc.contributor.authorJiang, L
 
dc.contributor.authorWang, DM
 
dc.contributor.authorYuan, J
 
dc.contributor.authorShen, JG
 
dc.contributor.authorYang, DP
 
dc.contributor.authorChen, JQ
 
dc.date.accessioned2010-10-06T03:47:15Z
 
dc.date.available2010-10-06T03:47:15Z
 
dc.date.issued2010
 
dc.description.abstractConjugated linoleic acid (CLA) has been shown to reduce body fat mass in various experimental animals. It is valuable to identify its influence on enzymes involved in energy expenditure, apoptosis, fatty acid oxidation and lipolysis. We investigated isomer-specific effects of high dose, long treatment of CLA (75.4 Μmol/L, 8 days) on protein and gene expression of these enzymes in cultured 3T3-L1 cells. Proteomics identified significant up- or down-regulation of 52 proteins by either CLA isomer. Protein and gene expression of uncoupling protein (UCP) 1, UCP3, perilipin and peroxisome proliferator-activated receptor (PPAR) α increased whereas UCP2 reduced for both CLA isomers. And eight-day treatment of trans-10,. cis-12 CLA, but not cis-9,. trans-11 CLA, significantly up-regulated protein and mRNA levels of PKA (P<05), CPT-1 and TNF-α (P<01). Compared to protein expression, both isomers did not significantly influence the mRNA expression of HSL, ATGL, ACO and leptin. In conclusion, high-dose, long treatment of cis-9,. trans-11 CLA did not promote apoptosis, fatty acid oxidation and lipolysis in adipocytes, but may induce an increase in energy expenditure. trans-10,. cis-12 CLA exhibited greater influence on lipid metabolism, stimulated adipocyte energy expenditure, apoptosis and fatty acid oxidation, but its effect on lipolysis was not obvious. © 2010 Elsevier Inc.
 
dc.description.naturepostprint
 
dc.identifier.citationJournal Of Nutritional Biochemistry, 2010, v. 21 n. 11, p. 1099-1105 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jnutbio.2009.09.007
 
dc.identifier.citeulike6641884
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.jnutbio.2009.09.007
 
dc.identifier.epage1105
 
dc.identifier.hkuros172662
 
dc.identifier.isiWOS:000283825600010
 
dc.identifier.issn0955-2863
2013 Impact Factor: 4.592
2013 SCImago Journal Rankings: 1.627
 
dc.identifier.issue11
 
dc.identifier.openurl
 
dc.identifier.pmid20138494
 
dc.identifier.scopuseid_2-s2.0-77955276240
 
dc.identifier.spage1099
 
dc.identifier.urihttp://hdl.handle.net/10722/123863
 
dc.identifier.volume21
 
dc.languageeng
 
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/jnutbio
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Nutritional Biochemistry
 
dc.relation.referencesReferences in Scopus
 
dc.rightsThe Journal of Nutritional Biochemistry. Copyright © Elsevier Inc.
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject3T3-L1
 
dc.subjectConjugated linoleic acid
 
dc.subjectLipid metabolism
 
dc.titleDifferent mechanisms of cis-9,trans-11- and trans-10,cis-12- conjugated linoleic acid affecting lipid metabolism in 3T3-L1 cells
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Zhongshan Ophthalmic Center
  3. Sun Yat-Sen University
  4. North University of China