Article: Different mechanisms of cis-9,trans-11- and trans-10,cis-12- conjugated linoleic acid affecting lipid metabolism in 3T3-L1 cells

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TitleDifferent mechanisms of cis-9,trans-11- and trans-10,cis-12- conjugated linoleic acid affecting lipid metabolism in 3T3-L1 cells
AuthorsZhai, JJ2 3
Liu, ZL4
Li, JM3
Chen, JP1
Jiang, L3
Wang, DM3
Yuan, J2
Shen, JG1
Yang, DP3
Chen, JQ2
Keywords3T3-L1
Conjugated linoleic acid
Lipid metabolism
Issue Date2010
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/jnutbio
CitationJournal Of Nutritional Biochemistry, 2010, v. 21 n. 11, p. 1099-1105 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jnutbio.2009.09.007
AbstractConjugated linoleic acid (CLA) has been shown to reduce body fat mass in various experimental animals. It is valuable to identify its influence on enzymes involved in energy expenditure, apoptosis, fatty acid oxidation and lipolysis. We investigated isomer-specific effects of high dose, long treatment of CLA (75.4 Μmol/L, 8 days) on protein and gene expression of these enzymes in cultured 3T3-L1 cells. Proteomics identified significant up- or down-regulation of 52 proteins by either CLA isomer. Protein and gene expression of uncoupling protein (UCP) 1, UCP3, perilipin and peroxisome proliferator-activated receptor (PPAR) α increased whereas UCP2 reduced for both CLA isomers. And eight-day treatment of trans-10,. cis-12 CLA, but not cis-9,. trans-11 CLA, significantly up-regulated protein and mRNA levels of PKA (P<05), CPT-1 and TNF-α (P<01). Compared to protein expression, both isomers did not significantly influence the mRNA expression of HSL, ATGL, ACO and leptin. In conclusion, high-dose, long treatment of cis-9,. trans-11 CLA did not promote apoptosis, fatty acid oxidation and lipolysis in adipocytes, but may induce an increase in energy expenditure. trans-10,. cis-12 CLA exhibited greater influence on lipid metabolism, stimulated adipocyte energy expenditure, apoptosis and fatty acid oxidation, but its effect on lipolysis was not obvious. © 2010 Elsevier Inc.
ISSN0955-2863
2011 Impact Factor: 3.891
2011 SCImago Journal Rankings: 0.233
DOIhttp://dx.doi.org/10.1016/j.jnutbio.2009.09.007
ISI Accession Number IDWOS:000283825600010
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorZhai, JJ
dc.contributor.authorLiu, ZL
dc.contributor.authorLi, JM
dc.contributor.authorChen, JP
dc.contributor.authorJiang, L
dc.contributor.authorWang, DM
dc.contributor.authorYuan, J
dc.contributor.authorShen, JG
dc.contributor.authorYang, DP
dc.contributor.authorChen, JQ
dc.date.accessioned2010-10-06T03:47:15Z
dc.date.available2010-10-06T03:47:15Z
dc.date.issued2010
dc.description.abstractConjugated linoleic acid (CLA) has been shown to reduce body fat mass in various experimental animals. It is valuable to identify its influence on enzymes involved in energy expenditure, apoptosis, fatty acid oxidation and lipolysis. We investigated isomer-specific effects of high dose, long treatment of CLA (75.4 Μmol/L, 8 days) on protein and gene expression of these enzymes in cultured 3T3-L1 cells. Proteomics identified significant up- or down-regulation of 52 proteins by either CLA isomer. Protein and gene expression of uncoupling protein (UCP) 1, UCP3, perilipin and peroxisome proliferator-activated receptor (PPAR) α increased whereas UCP2 reduced for both CLA isomers. And eight-day treatment of trans-10,. cis-12 CLA, but not cis-9,. trans-11 CLA, significantly up-regulated protein and mRNA levels of PKA (P<05), CPT-1 and TNF-α (P<01). Compared to protein expression, both isomers did not significantly influence the mRNA expression of HSL, ATGL, ACO and leptin. In conclusion, high-dose, long treatment of cis-9,. trans-11 CLA did not promote apoptosis, fatty acid oxidation and lipolysis in adipocytes, but may induce an increase in energy expenditure. trans-10,. cis-12 CLA exhibited greater influence on lipid metabolism, stimulated adipocyte energy expenditure, apoptosis and fatty acid oxidation, but its effect on lipolysis was not obvious. © 2010 Elsevier Inc.
dc.description.naturepostprint
dc.identifier.citationJournal Of Nutritional Biochemistry, 2010, v. 21 n. 11, p. 1099-1105 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jnutbio.2009.09.007
dc.identifier.citeulike6641884
dc.identifier.doihttp://dx.doi.org/10.1016/j.jnutbio.2009.09.007
dc.identifier.epage1105
dc.identifier.hkuros172662
dc.identifier.isiWOS:000283825600010
dc.identifier.issn0955-2863
2011 Impact Factor: 3.891
2011 SCImago Journal Rankings: 0.233
dc.identifier.issue11
dc.identifier.openurl
dc.identifier.pmid20138494
dc.identifier.scopuseid_2-s2.0-77955276240
dc.identifier.spage1099
dc.identifier.urihttp://hdl.handle.net/10722/123863
dc.identifier.volume21
dc.languageeng
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/jnutbio
dc.publisher.placeUnited States
dc.relation.ispartofJournal of Nutritional Biochemistry
dc.relation.referencesReferences in Scopus
dc.rightsThe Journal of Nutritional Biochemistry. Copyright © Elsevier Inc.
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
dc.subject3T3-L1
dc.subjectConjugated linoleic acid
dc.subjectLipid metabolism
dc.titleDifferent mechanisms of cis-9,trans-11- and trans-10,cis-12- conjugated linoleic acid affecting lipid metabolism in 3T3-L1 cells
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Zhongshan Ophthalmic Center
  3. Sun Yat-Sen University
  4. North University of China