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Article: Different mechanisms of cis-9,trans-11- and trans-10,cis-12- conjugated linoleic acid affecting lipid metabolism in 3T3-L1 cells

TitleDifferent mechanisms of cis-9,trans-11- and trans-10,cis-12- conjugated linoleic acid affecting lipid metabolism in 3T3-L1 cells
Authors
Keywords3T3-L1
Conjugated linoleic acid
Lipid metabolism
Issue Date2010
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/jnutbio
Citation
Journal Of Nutritional Biochemistry, 2010, v. 21 n. 11, p. 1099-1105 How to Cite?
AbstractConjugated linoleic acid (CLA) has been shown to reduce body fat mass in various experimental animals. It is valuable to identify its influence on enzymes involved in energy expenditure, apoptosis, fatty acid oxidation and lipolysis. We investigated isomer-specific effects of high dose, long treatment of CLA (75.4 Μmol/L, 8 days) on protein and gene expression of these enzymes in cultured 3T3-L1 cells. Proteomics identified significant up- or down-regulation of 52 proteins by either CLA isomer. Protein and gene expression of uncoupling protein (UCP) 1, UCP3, perilipin and peroxisome proliferator-activated receptor (PPAR) α increased whereas UCP2 reduced for both CLA isomers. And eight-day treatment of trans-10,. cis-12 CLA, but not cis-9,. trans-11 CLA, significantly up-regulated protein and mRNA levels of PKA (P<05), CPT-1 and TNF-α (P<01). Compared to protein expression, both isomers did not significantly influence the mRNA expression of HSL, ATGL, ACO and leptin. In conclusion, high-dose, long treatment of cis-9,. trans-11 CLA did not promote apoptosis, fatty acid oxidation and lipolysis in adipocytes, but may induce an increase in energy expenditure. trans-10,. cis-12 CLA exhibited greater influence on lipid metabolism, stimulated adipocyte energy expenditure, apoptosis and fatty acid oxidation, but its effect on lipolysis was not obvious. © 2010 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/123863
ISSN
2015 Impact Factor: 4.668
2015 SCImago Journal Rankings: 1.886
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhai, JJen_HK
dc.contributor.authorLiu, ZLen_HK
dc.contributor.authorLi, JMen_HK
dc.contributor.authorChen, JPen_HK
dc.contributor.authorJiang, Len_HK
dc.contributor.authorWang, DMen_HK
dc.contributor.authorYuan, Jen_HK
dc.contributor.authorShen, JGen_HK
dc.contributor.authorYang, DPen_HK
dc.contributor.authorChen, JQen_HK
dc.date.accessioned2010-10-06T03:47:15Z-
dc.date.available2010-10-06T03:47:15Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Nutritional Biochemistry, 2010, v. 21 n. 11, p. 1099-1105en_HK
dc.identifier.issn0955-2863en_HK
dc.identifier.urihttp://hdl.handle.net/10722/123863-
dc.description.abstractConjugated linoleic acid (CLA) has been shown to reduce body fat mass in various experimental animals. It is valuable to identify its influence on enzymes involved in energy expenditure, apoptosis, fatty acid oxidation and lipolysis. We investigated isomer-specific effects of high dose, long treatment of CLA (75.4 Μmol/L, 8 days) on protein and gene expression of these enzymes in cultured 3T3-L1 cells. Proteomics identified significant up- or down-regulation of 52 proteins by either CLA isomer. Protein and gene expression of uncoupling protein (UCP) 1, UCP3, perilipin and peroxisome proliferator-activated receptor (PPAR) α increased whereas UCP2 reduced for both CLA isomers. And eight-day treatment of trans-10,. cis-12 CLA, but not cis-9,. trans-11 CLA, significantly up-regulated protein and mRNA levels of PKA (P<05), CPT-1 and TNF-α (P<01). Compared to protein expression, both isomers did not significantly influence the mRNA expression of HSL, ATGL, ACO and leptin. In conclusion, high-dose, long treatment of cis-9,. trans-11 CLA did not promote apoptosis, fatty acid oxidation and lipolysis in adipocytes, but may induce an increase in energy expenditure. trans-10,. cis-12 CLA exhibited greater influence on lipid metabolism, stimulated adipocyte energy expenditure, apoptosis and fatty acid oxidation, but its effect on lipolysis was not obvious. © 2010 Elsevier Inc.en_HK
dc.languageeng-
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/jnutbioen_HK
dc.relation.ispartofJournal of Nutritional Biochemistryen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThe Journal of Nutritional Biochemistry. Copyright © Elsevier Inc.-
dc.subject3T3-L1en_HK
dc.subjectConjugated linoleic aciden_HK
dc.subjectLipid metabolismen_HK
dc.titleDifferent mechanisms of cis-9,trans-11- and trans-10,cis-12- conjugated linoleic acid affecting lipid metabolism in 3T3-L1 cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0955-2863&volume=&spage=In Press, Corrected Proof&epage=&date=2010&atitle=Different+mechanisms+of+cis-9,trans-11-+and+trans-10,cis-12-+conjugated+linoleic+acid+affecting+lipid+metabolism+in+3T3-L1+cells-
dc.identifier.emailChen, JP: abchen@hku.hken_HK
dc.identifier.emailShen, JG: shenjg@hku.hken_HK
dc.identifier.authorityChen, JP=rp01316en_HK
dc.identifier.authorityShen, JG=rp00487en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.jnutbio.2009.09.007en_HK
dc.identifier.pmid20138494-
dc.identifier.scopuseid_2-s2.0-77955276240en_HK
dc.identifier.hkuros172662-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77955276240&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume21en_HK
dc.identifier.issue11en_HK
dc.identifier.spage1099en_HK
dc.identifier.epage1105en_HK
dc.identifier.isiWOS:000283825600010-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhai, JJ=24172902900en_HK
dc.identifier.scopusauthoridLiu, ZL=37031473600en_HK
dc.identifier.scopusauthoridLi, JM=36064381400en_HK
dc.identifier.scopusauthoridChen, JP=22733695400en_HK
dc.identifier.scopusauthoridJiang, L=55197796100en_HK
dc.identifier.scopusauthoridWang, DM=7407071911en_HK
dc.identifier.scopusauthoridYuan, J=35276777500en_HK
dc.identifier.scopusauthoridShen, JG=7404929947en_HK
dc.identifier.scopusauthoridYang, DP=22959396400en_HK
dc.identifier.scopusauthoridChen, JQ=8394238000en_HK
dc.identifier.citeulike6641884-

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