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Article: The post-antifungal effect (PAFE) of amphotericin B, nystatin, ketoconazole and 5-fluorocytosine and its impact on the colonization traits of Candida glabrata

TitleThe post-antifungal effect (PAFE) of amphotericin B, nystatin, ketoconazole and 5-fluorocytosine and its impact on the colonization traits of Candida glabrata
Authors
KeywordsAntifungals
Candida glabrata
Colonization
PAFE
Virulence
Issue Date2010
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/13693786.asp
Citation
Medical Mycology, 2010, v. 48 n. 5, p. 725-734 How to Cite?
AbstractThe post-antifungal effect (PAFE) has been shown to affect Candida pathogenicity, but there is little information on either PAFE or its association with the colonization traits of Candida glabrata. The objective of this study was to determine, in vitro, the PAFE on 14 C. glabrata isolates following exposure to amphotericin B (AMB), nystatin (NYS), ketoconazole (KETO) and 5-fluorocytosine (5FC). In addition, we evaluated the impact of PAFE on yeast adherence to buccal epithelial cells (BEC), cell-surface-hydrophobicity (CSH) and biofilm growth (BG) on denture acrylic surfaces. PAFE was induced following a 1-h exposure of yeasts to (×1×4MIC) of AMB, NYS, KETO and 5FC in RPMI medium and, measured using automated turbidometry. The BEC adhesion, CSH and BG assays were performed by the methods of Kimura & Pearsall, Sweet et al., and Jin et al., respectively. Significant differences in PAFE (P < 0.001) were observed after exposure to AMB and NYS, but not KETO and 5FC. Following exposure to AMB, NYS, KETO and 5FC, significant inter-strain differences (P < 0.001) were observed in percentage terms in adhesion (39.0%, 43.48%, 38.28%, 35.07%) and biofilm growth (42.86%, 39.86%, 42.81%, 36.38%), respectively. Short exposure of C. glabrata to sub-cidal concentrations of antifungals modulates yeast growth and also affects some of their colonization traits. © 2010 ISHAM.
Persistent Identifierhttp://hdl.handle.net/10722/123852
ISSN
2015 Impact Factor: 2.644
2015 SCImago Journal Rankings: 1.055
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council of the Hong Kong SAR, China10205959
Funding Information:

The authors thank A.P.K. Wong for excellent technical support. The work described in this paper was partially supported by a grant from the Research Grants Council of the Hong Kong SAR, China (Grant No. 10205959).

References

 

DC FieldValueLanguage
dc.contributor.authorSamaranayake, YHen_HK
dc.contributor.authorYau, JYYen_HK
dc.contributor.authorThein, ZMen_HK
dc.contributor.authorJayatilake, JAMSen_HK
dc.contributor.authorYeung, KWSen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-10-05T02:40:42Z-
dc.date.available2010-10-05T02:40:42Z-
dc.date.issued2010en_HK
dc.identifier.citationMedical Mycology, 2010, v. 48 n. 5, p. 725-734en_HK
dc.identifier.issn1369-3786en_HK
dc.identifier.urihttp://hdl.handle.net/10722/123852-
dc.description.abstractThe post-antifungal effect (PAFE) has been shown to affect Candida pathogenicity, but there is little information on either PAFE or its association with the colonization traits of Candida glabrata. The objective of this study was to determine, in vitro, the PAFE on 14 C. glabrata isolates following exposure to amphotericin B (AMB), nystatin (NYS), ketoconazole (KETO) and 5-fluorocytosine (5FC). In addition, we evaluated the impact of PAFE on yeast adherence to buccal epithelial cells (BEC), cell-surface-hydrophobicity (CSH) and biofilm growth (BG) on denture acrylic surfaces. PAFE was induced following a 1-h exposure of yeasts to (×1×4MIC) of AMB, NYS, KETO and 5FC in RPMI medium and, measured using automated turbidometry. The BEC adhesion, CSH and BG assays were performed by the methods of Kimura & Pearsall, Sweet et al., and Jin et al., respectively. Significant differences in PAFE (P < 0.001) were observed after exposure to AMB and NYS, but not KETO and 5FC. Following exposure to AMB, NYS, KETO and 5FC, significant inter-strain differences (P < 0.001) were observed in percentage terms in adhesion (39.0%, 43.48%, 38.28%, 35.07%) and biofilm growth (42.86%, 39.86%, 42.81%, 36.38%), respectively. Short exposure of C. glabrata to sub-cidal concentrations of antifungals modulates yeast growth and also affects some of their colonization traits. © 2010 ISHAM.en_HK
dc.languageeng-
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/13693786.aspen_HK
dc.relation.ispartofMedical Mycologyen_HK
dc.rightsMedical Mycology. Copyright © Informa Healthcare.-
dc.subjectAntifungalsen_HK
dc.subjectCandida glabrataen_HK
dc.subjectColonizationen_HK
dc.subjectPAFEen_HK
dc.subjectVirulenceen_HK
dc.titleThe post-antifungal effect (PAFE) of amphotericin B, nystatin, ketoconazole and 5-fluorocytosine and its impact on the colonization traits of Candida glabrataen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1369-3786&volume=48&issue=5&spage=725&epage=734&date=2010&atitle=The+post-antifungal+effect+(PAFE)+of+amphotericin+B,+nystatin,+ketoconazole+and+5-fluorocytosine+and+its+impact+on+the+colonization+traits+of+Candida+glabrata-
dc.identifier.emailSamaranayake, YH:hema@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, YH=rp00025en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3109/13693780903496583en_HK
dc.identifier.pmid20092419-
dc.identifier.scopuseid_2-s2.0-77954810938en_HK
dc.identifier.hkuros171958-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77954810938&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume48en_HK
dc.identifier.issue5en_HK
dc.identifier.spage725en_HK
dc.identifier.epage734en_HK
dc.identifier.isiWOS:000280700800007-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridSamaranayake, YH=6602677237en_HK
dc.identifier.scopusauthoridYau, JYY=7102167568en_HK
dc.identifier.scopusauthoridThein, ZM=14049367300en_HK
dc.identifier.scopusauthoridJayatilake, JAMS=8441671500en_HK
dc.identifier.scopusauthoridYeung, KWS=25931923800en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK

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