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Article: Differential NOD/SCID mouse engraftment of peripheral blood CD34 + cells and JAK2V617F clones from patients with myeloproliferative neoplasms

TitleDifferential NOD/SCID mouse engraftment of peripheral blood CD34 + cells and JAK2V617F clones from patients with myeloproliferative neoplasms
Authors
KeywordsJAK2
Myeloproliferative disease
NOD/SCID mice
Polycythemia vera
Issue Date2010
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/leukres
Citation
Leukemia Research, 2010, v. 34 n. 10, p. 1390-1394 How to Cite?
Abstract
We evaluated the NOD/SCID engraftment of CD34 + cells from polycythemia vera (PV) and secondary polycythemia patients (SP) and the JAK2V617F clone before and after transplantation. Peripheral blood CD34 + cells were transplanted intra-femorally. In the injected BM, successful engraftment (>0.1%) occurred in 8/26 mice transplanted with CD34+ cells from 5/13 PV patients (median: 4.26%, range: 0.3-5.56%), in contrast to 0/14 mice from 9 SP patients (P=0.017). The engrafting PV cells were of multi-lineage. JAK2V617F/total JAK2 ratios decreased after transplantation (initial: 65.9% versus 6-week: 13.0%, P=0.001). Essential thrombocythemia (ET) BM cells also exhibited a similar decrease in JAK2V617F clone. The results suggested that events in addition to JAK2V617F are involved in the pathogenesis of PV and ET. © 2010 Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/123827
ISSN
2013 Impact Factor: 2.692
2013 SCImago Journal Rankings: 1.080
ISI Accession Number ID
Funding AgencyGrant Number
RGCHKU 7488/04M
7520/06M
CRCG (HKU)
HKU
Funding Information:

This work was supported by RGC grant (HKU 7488/04M and 7520/06M), a small project funding from CRCG (HKU) and a grant from the Strategic Research Theme on Cancer Stem Cells from HKU. We were grateful to Dr. Harold K.K. Lee, Associate Consultant and the Haematology Team, Princess Margaret Hospital, Hong Kong, for providing us with the venesection samples from some patients.

References
Grants

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorFung, TKen_HK
dc.contributor.authorCheung, AMSen_HK
dc.contributor.authorKwong, YLen_HK
dc.contributor.authorLiang, Ren_HK
dc.contributor.authorLeung, AYHen_HK
dc.date.accessioned2010-09-29T06:14:01Z-
dc.date.available2010-09-29T06:14:01Z-
dc.date.issued2010en_HK
dc.identifier.citationLeukemia Research, 2010, v. 34 n. 10, p. 1390-1394en_HK
dc.identifier.issn0145-2126en_HK
dc.identifier.urihttp://hdl.handle.net/10722/123827-
dc.description.abstractWe evaluated the NOD/SCID engraftment of CD34 + cells from polycythemia vera (PV) and secondary polycythemia patients (SP) and the JAK2V617F clone before and after transplantation. Peripheral blood CD34 + cells were transplanted intra-femorally. In the injected BM, successful engraftment (>0.1%) occurred in 8/26 mice transplanted with CD34+ cells from 5/13 PV patients (median: 4.26%, range: 0.3-5.56%), in contrast to 0/14 mice from 9 SP patients (P=0.017). The engrafting PV cells were of multi-lineage. JAK2V617F/total JAK2 ratios decreased after transplantation (initial: 65.9% versus 6-week: 13.0%, P=0.001). Essential thrombocythemia (ET) BM cells also exhibited a similar decrease in JAK2V617F clone. The results suggested that events in addition to JAK2V617F are involved in the pathogenesis of PV and ET. © 2010 Elsevier Ltd.en_HK
dc.languageeng-
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/leukresen_HK
dc.relation.ispartofLeukemia Researchen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectJAK2en_HK
dc.subjectMyeloproliferative diseaseen_HK
dc.subjectNOD/SCID miceen_HK
dc.subjectPolycythemia veraen_HK
dc.titleDifferential NOD/SCID mouse engraftment of peripheral blood CD34 + cells and JAK2V617F clones from patients with myeloproliferative neoplasmsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0145-2126&volume=34&issue=10&spage=1390&epage=1394&date=2010&atitle=Differential+NOD/SCID+mouse+engraftment+of+peripheral+blood+CD34(+)+cells+and+JAK2V617F+clones+from+patients+with+myeloproliferative+neoplasms-
dc.identifier.emailCheung, AMS:h9945256@graduate.hku.hken_HK
dc.identifier.emailKwong, YL:ylkwong@hku.hken_HK
dc.identifier.emailLiang, R:rliang@hku.hken_HK
dc.identifier.emailLeung, AYH:ayhleung@hku.hken_HK
dc.identifier.authorityCheung, AMS=rp01572en_HK
dc.identifier.authorityKwong, YL=rp00358en_HK
dc.identifier.authorityLiang, R=rp00345en_HK
dc.identifier.authorityLeung, AYH=rp00265en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.leukres.2010.01.028en_HK
dc.identifier.pmid20170959en_HK
dc.identifier.scopuseid_2-s2.0-77955984222en_HK
dc.identifier.hkuros173552-
dc.identifier.hkuros200896-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77955984222&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume34en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1390en_HK
dc.identifier.epage1394en_HK
dc.identifier.isiWOS:000281214700022-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectA study of normal and deregulated haematopoiesis in zebrafish, with special reference to the role of BMP signaling in haematopoietic stem cell proliferation-
dc.identifier.scopusauthoridFung, TK=7102715924en_HK
dc.identifier.scopusauthoridCheung, AMS=36985759800en_HK
dc.identifier.scopusauthoridKwong, YL=7102818954en_HK
dc.identifier.scopusauthoridLiang, R=26643224900en_HK
dc.identifier.scopusauthoridLeung, AYH=7403012668en_HK
dc.identifier.citeulike6866170-

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