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Article: Differential NOD/SCID mouse engraftment of peripheral blood CD34 + cells and JAK2V617F clones from patients with myeloproliferative neoplasms
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TitleDifferential NOD/SCID mouse engraftment of peripheral blood CD34 + cells and JAK2V617F clones from patients with myeloproliferative neoplasms
 
AuthorsFung, TK1
Cheung, AMS1
Kwong, YL1
Liang, R1
Leung, AYH1
 
KeywordsJAK2
Myeloproliferative disease
NOD/SCID mice
Polycythemia vera
 
Issue Date2010
 
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/leukres
 
CitationLeukemia Research, 2010, v. 34 n. 10, p. 1390-1394 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.leukres.2010.01.028
 
AbstractWe evaluated the NOD/SCID engraftment of CD34 + cells from polycythemia vera (PV) and secondary polycythemia patients (SP) and the JAK2V617F clone before and after transplantation. Peripheral blood CD34 + cells were transplanted intra-femorally. In the injected BM, successful engraftment (>0.1%) occurred in 8/26 mice transplanted with CD34+ cells from 5/13 PV patients (median: 4.26%, range: 0.3-5.56%), in contrast to 0/14 mice from 9 SP patients (P=0.017). The engrafting PV cells were of multi-lineage. JAK2V617F/total JAK2 ratios decreased after transplantation (initial: 65.9% versus 6-week: 13.0%, P=0.001). Essential thrombocythemia (ET) BM cells also exhibited a similar decrease in JAK2V617F clone. The results suggested that events in addition to JAK2V617F are involved in the pathogenesis of PV and ET. © 2010 Elsevier Ltd.
 
ISSN0145-2126
2013 Impact Factor: 2.692
2013 SCImago Journal Rankings: 1.080
 
DOIhttp://dx.doi.org/10.1016/j.leukres.2010.01.028
 
ISI Accession Number IDWOS:000281214700022
Funding AgencyGrant Number
RGCHKU 7488/04M
7520/06M
CRCG (HKU)
HKU
Funding Information:

This work was supported by RGC grant (HKU 7488/04M and 7520/06M), a small project funding from CRCG (HKU) and a grant from the Strategic Research Theme on Cancer Stem Cells from HKU. We were grateful to Dr. Harold K.K. Lee, Associate Consultant and the Haematology Team, Princess Margaret Hospital, Hong Kong, for providing us with the venesection samples from some patients.

 
ReferencesReferences in Scopus
 
GrantsA study of normal and deregulated haematopoiesis in zebrafish, with special reference to the role of BMP signaling in haematopoietic stem cell proliferation
 
DC FieldValue
dc.contributor.authorFung, TK
 
dc.contributor.authorCheung, AMS
 
dc.contributor.authorKwong, YL
 
dc.contributor.authorLiang, R
 
dc.contributor.authorLeung, AYH
 
dc.date.accessioned2010-09-29T06:14:01Z
 
dc.date.available2010-09-29T06:14:01Z
 
dc.date.issued2010
 
dc.description.abstractWe evaluated the NOD/SCID engraftment of CD34 + cells from polycythemia vera (PV) and secondary polycythemia patients (SP) and the JAK2V617F clone before and after transplantation. Peripheral blood CD34 + cells were transplanted intra-femorally. In the injected BM, successful engraftment (>0.1%) occurred in 8/26 mice transplanted with CD34+ cells from 5/13 PV patients (median: 4.26%, range: 0.3-5.56%), in contrast to 0/14 mice from 9 SP patients (P=0.017). The engrafting PV cells were of multi-lineage. JAK2V617F/total JAK2 ratios decreased after transplantation (initial: 65.9% versus 6-week: 13.0%, P=0.001). Essential thrombocythemia (ET) BM cells also exhibited a similar decrease in JAK2V617F clone. The results suggested that events in addition to JAK2V617F are involved in the pathogenesis of PV and ET. © 2010 Elsevier Ltd.
 
dc.description.naturepostprint
 
dc.identifier.citationLeukemia Research, 2010, v. 34 n. 10, p. 1390-1394 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.leukres.2010.01.028
 
dc.identifier.citeulike6866170
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.leukres.2010.01.028
 
dc.identifier.epage1394
 
dc.identifier.hkuros173552
 
dc.identifier.hkuros200896
 
dc.identifier.isiWOS:000281214700022
Funding AgencyGrant Number
RGCHKU 7488/04M
7520/06M
CRCG (HKU)
HKU
Funding Information:

This work was supported by RGC grant (HKU 7488/04M and 7520/06M), a small project funding from CRCG (HKU) and a grant from the Strategic Research Theme on Cancer Stem Cells from HKU. We were grateful to Dr. Harold K.K. Lee, Associate Consultant and the Haematology Team, Princess Margaret Hospital, Hong Kong, for providing us with the venesection samples from some patients.

 
dc.identifier.issn0145-2126
2013 Impact Factor: 2.692
2013 SCImago Journal Rankings: 1.080
 
dc.identifier.issue10
 
dc.identifier.openurl
 
dc.identifier.pmid20170959
 
dc.identifier.scopuseid_2-s2.0-77955984222
 
dc.identifier.spage1390
 
dc.identifier.urihttp://hdl.handle.net/10722/123827
 
dc.identifier.volume34
 
dc.languageeng
 
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/leukres
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofLeukemia Research
 
dc.relation.projectA study of normal and deregulated haematopoiesis in zebrafish, with special reference to the role of BMP signaling in haematopoietic stem cell proliferation
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subjectJAK2
 
dc.subjectMyeloproliferative disease
 
dc.subjectNOD/SCID mice
 
dc.subjectPolycythemia vera
 
dc.titleDifferential NOD/SCID mouse engraftment of peripheral blood CD34 + cells and JAK2V617F clones from patients with myeloproliferative neoplasms
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong