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Article: Successful engraftment by leukemia initiating cells in adult acute lymphoblastic leukemia after direct intrahepatic injection into unconditioned newborn NOD/SCID mice

TitleSuccessful engraftment by leukemia initiating cells in adult acute lymphoblastic leukemia after direct intrahepatic injection into unconditioned newborn NOD/SCID mice
Authors
Issue Date2010
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/exphem
Citation
Experimental Hematology, 2010, v. 38 n. 1, p. 3-10 How to Cite?
AbstractObjective: Xenogeneic transplantation has been the gold standard for enumeration of leukemia initiating cells in acute myeloid and lymphoblastic leukemia (ALL). Most transplantation models have required conditioning in which the recipients were either irradiated or treated with chemotherapy prior to injection of human leukemia cells. In this study, we reported an undescribed model in which adult ALL cells were injected into unconditioned newborn nonobese diabetic severe combined immunodeficient mice via an intrahepatic route. Materials and Methods: Bone marrow (BM) and peripheral blood were collected from patients with ALL at diagnosis or relapse. CD34 + selected lymphoblasts or mononuclear cells were transplanted as mentioned previously. Cells were also transplanted into sublethally irradiated adult mice via intravenous route for comparison. Leukemia engraftment was enumerated from mouse BM 6 to 18 weeks after transplantation. Clonality of the engrafting cells was examined based on IGH rearrangement and fluorescent in situ hybridization. Results: Five of 13 ALL samples engrafted into the recipient BM 6 to 18 weeks after transplantation. Engrafted cells recapitulated the immunophenotype and cytogenetic characteristics of the original samples. Engraftment in BM and peripheral blood was significantly correlated. Importantly, there was significant correlation of engraftment between this and the conventional adult nonobese diabetic severe combined immunodeficient mouse model involving irradiation. Conclusion: Our results demonstrated that this unconditioned newborn mouse model could be used for enumeration of leukemia initiating cells in ALL and should be further evaluated. © 2010 ISEH - Society for Hematology and Stem Cells.
Persistent Identifierhttp://hdl.handle.net/10722/123826
ISSN
2015 Impact Factor: 2.303
2015 SCImago Journal Rankings: 1.341
ISI Accession Number ID
Funding AgencyGrant Number
RGCHKU 7488/04M
7520/06M
Internal Research Funding Programme (HKU)
Strategy Research Theme oil cancer stein cells of the LKS Faculty of Medicine (HKU) (Hong Kong, China)
Funding Information:

This work was supported by RGC grant (HKU 7488/04M and 7520/06M), a small project funding from Internal Research Funding Programme (HKU), and the Strategy Research Theme oil cancer stein cells of the LKS Faculty of Medicine (HKU) (Hong Kong, China).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorCheung, AMSen_HK
dc.contributor.authorFung, TKen_HK
dc.contributor.authorFan, AKPen_HK
dc.contributor.authorWan, TSKen_HK
dc.contributor.authorChow, HCHen_HK
dc.contributor.authorLeung, JCKen_HK
dc.contributor.authorChan, LYYen_HK
dc.contributor.authorKwong, YLen_HK
dc.contributor.authorLiang, Ren_HK
dc.contributor.authorLeung, AYHen_HK
dc.date.accessioned2010-09-29T04:23:06Z-
dc.date.available2010-09-29T04:23:06Z-
dc.date.issued2010en_HK
dc.identifier.citationExperimental Hematology, 2010, v. 38 n. 1, p. 3-10en_HK
dc.identifier.issn0301-472Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/123826-
dc.description.abstractObjective: Xenogeneic transplantation has been the gold standard for enumeration of leukemia initiating cells in acute myeloid and lymphoblastic leukemia (ALL). Most transplantation models have required conditioning in which the recipients were either irradiated or treated with chemotherapy prior to injection of human leukemia cells. In this study, we reported an undescribed model in which adult ALL cells were injected into unconditioned newborn nonobese diabetic severe combined immunodeficient mice via an intrahepatic route. Materials and Methods: Bone marrow (BM) and peripheral blood were collected from patients with ALL at diagnosis or relapse. CD34 + selected lymphoblasts or mononuclear cells were transplanted as mentioned previously. Cells were also transplanted into sublethally irradiated adult mice via intravenous route for comparison. Leukemia engraftment was enumerated from mouse BM 6 to 18 weeks after transplantation. Clonality of the engrafting cells was examined based on IGH rearrangement and fluorescent in situ hybridization. Results: Five of 13 ALL samples engrafted into the recipient BM 6 to 18 weeks after transplantation. Engrafted cells recapitulated the immunophenotype and cytogenetic characteristics of the original samples. Engraftment in BM and peripheral blood was significantly correlated. Importantly, there was significant correlation of engraftment between this and the conventional adult nonobese diabetic severe combined immunodeficient mouse model involving irradiation. Conclusion: Our results demonstrated that this unconditioned newborn mouse model could be used for enumeration of leukemia initiating cells in ALL and should be further evaluated. © 2010 ISEH - Society for Hematology and Stem Cells.en_HK
dc.languageeng-
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/exphemen_HK
dc.relation.ispartofExperimental Hematologyen_HK
dc.rightsExperimental Hematology. Copyright © Elsevier Inc.-
dc.subject.meshAdult-
dc.subject.meshAnimals-
dc.subject.meshAnimals, Newborn-
dc.subject.meshNeoplasm Transplantation-
dc.subject.meshPrecursor Cell Lymphoblastic Leukemia-Lymphoma - pathology-
dc.titleSuccessful engraftment by leukemia initiating cells in adult acute lymphoblastic leukemia after direct intrahepatic injection into unconditioned newborn NOD/SCID miceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0301-472X&volume=38&issue=1&spage=3&epage=10&date=2010&atitle=Successful+engraftment+by+leukemia+initiating+cells+in+adult+acute+lymphoblastic+leukemia+after+direct+intrahepatic+injection+into+unconditioned+newborn+NOD/SCID+mice-
dc.identifier.emailCheung, AMS:h9945256@graduate.hku.hken_HK
dc.identifier.emailLeung, JCK:jckleung@hku.hken_HK
dc.identifier.emailKwong, YL:ylkwong@hku.hken_HK
dc.identifier.emailLiang, R:rliang@hku.hken_HK
dc.identifier.emailLeung, AYH:ayhleung@hku.hken_HK
dc.identifier.authorityCheung, AMS=rp01572en_HK
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.identifier.authorityKwong, YL=rp00358en_HK
dc.identifier.authorityLiang, R=rp00345en_HK
dc.identifier.authorityLeung, AYH=rp00265en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.exphem.2009.10.007en_HK
dc.identifier.pmid19837128-
dc.identifier.scopuseid_2-s2.0-71249084382en_HK
dc.identifier.hkuros173549-
dc.identifier.hkuros200897-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-71249084382&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume38en_HK
dc.identifier.issue1en_HK
dc.identifier.spage3en_HK
dc.identifier.epage10en_HK
dc.identifier.eissn1873-2399-
dc.identifier.isiWOS:000273142300002-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectA study of normal and deregulated haematopoiesis in zebrafish, with special reference to the role of BMP signaling in haematopoietic stem cell proliferation-
dc.identifier.scopusauthoridCheung, AMS=36985759800en_HK
dc.identifier.scopusauthoridFung, TK=7102715924en_HK
dc.identifier.scopusauthoridFan, AKP=35336410600en_HK
dc.identifier.scopusauthoridWan, TSK=25623981600en_HK
dc.identifier.scopusauthoridChow, HCH=7102303391en_HK
dc.identifier.scopusauthoridLeung, JCK=7202180349en_HK
dc.identifier.scopusauthoridChan, LYY=35336076700en_HK
dc.identifier.scopusauthoridKwong, YL=7102818954en_HK
dc.identifier.scopusauthoridLiang, R=26643224900en_HK
dc.identifier.scopusauthoridLeung, AYH=7403012668en_HK
dc.identifier.citeulike6001228-

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