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Article: Primate mandibular reconstruction with prefabricated, vascularized tissue-engineered bone flaps and recombinant human bone morphogenetic protein-2 implanted in situ

TitlePrimate mandibular reconstruction with prefabricated, vascularized tissue-engineered bone flaps and recombinant human bone morphogenetic protein-2 implanted in situ
Authors
KeywordsCoralline hydroxyapatite
Demineralized freeze-dried bone allograft
Mandibular reconstruction
Prefabricated vascularized bone flaps
RhBMP-2
Issue Date2010
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials
Citation
Biomaterials, 2010, v. 31 n. 18, p. 4935-4943 How to Cite?
AbstractSeveral studies have validated successful mandibular reconstruction with prefabricated tissue-engineered bone flaps and recombinant human bone morphogenetic protein-2 (rhBMP-2) implanted in situ. Whether rhBMP-2 applied with the prefabrication technique enables faster ossification of mandibular defects than rhBMP-2 applied in situ is unknown. We aimed to compare mandibular reconstruction with prefabricated, vascularized tissue-engineered bone flaps with rhBMP-2 and rhBMP-2 applied in situ in primates (Rhesus monkey). We also compared the use of the carriers demineralized freeze-dried bone allograft (DFDBA) and coralline hydroxyapatite (CHA) for applying rhBMP-2. After computed tomography of the monkey head, custom meshes were made, loaded with rhBMP-2-incorporated DFDBA or CHA, and implanted in the latissimus dorsi muscle. Meanwhile, contralateral segmental mandibular defects were created, and custom meshes loaded with DFDBA, CHA, or rhBMP-2-incooperated DFDBA and CHA were implanted in situ. Thirteen weeks later, the bone flaps with rhBMP-2-incorporated DFDBA or CHA were transferred to repair segmental mandibular defects. The meshes loaded with DFDBA or CHA alone showed no bone regeneration 13 weeks after implantation in latissimus dorsi muscle. Radiography, angiography and histological analysis were used to evaluate the repair and vascularization of the implant. Segmental mandibular defects were successfully restored with prefabricated bone flaps and rhBMP-2-incorporated CHA in situ, but other segmental mandibular defects remained with rhBMP-2-incorporated DFDBA, DFDBA and CHA in situ. Moreover, mandibles reconstructed with rhBMP-2-incorporated CHA bone flaps revealed more regenerated and homogeneous bone formation than did other reconstructions. The study suggested that the prefabrication technique induced better mandibular reconstruction and bone regeneration in quantity and quality. © 2010 Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/123819
ISSN
2015 Impact Factor: 8.387
2015 SCImago Journal Rankings: 3.565
ISI Accession Number ID
Funding AgencyGrant Number
Beijing Municipal Commission of Science and TechnologyD090600704040291
Funding Information:

This project was financially supported by the Beijing Municipal Commission of Science and Technology (D090600704040291). We thank the Ear, Nose and Throat Research Institute, Department of Stomatology, Department of Nuclear Medicine and Animal Center of Chinese PLA General Hospital for their generous help. We thank JIMAFEI Science and Technology Development Co. Ltd. for manufacture custom titanium meshes. We thank OsteoRad Biomaterial Co. Ltd. (China) for manufacture the DFDBA used in the study.

References

 

DC FieldValueLanguage
dc.contributor.authorZhou, Men_HK
dc.contributor.authorPeng, Xen_HK
dc.contributor.authorMao, Cen_HK
dc.contributor.authorXu, Fen_HK
dc.contributor.authorHu, Men_HK
dc.contributor.authorYu Guangyan, GYen_HK
dc.date.accessioned2010-09-28T03:58:10Z-
dc.date.available2010-09-28T03:58:10Z-
dc.date.issued2010en_HK
dc.identifier.citationBiomaterials, 2010, v. 31 n. 18, p. 4935-4943en_HK
dc.identifier.issn0142-9612en_HK
dc.identifier.urihttp://hdl.handle.net/10722/123819-
dc.description.abstractSeveral studies have validated successful mandibular reconstruction with prefabricated tissue-engineered bone flaps and recombinant human bone morphogenetic protein-2 (rhBMP-2) implanted in situ. Whether rhBMP-2 applied with the prefabrication technique enables faster ossification of mandibular defects than rhBMP-2 applied in situ is unknown. We aimed to compare mandibular reconstruction with prefabricated, vascularized tissue-engineered bone flaps with rhBMP-2 and rhBMP-2 applied in situ in primates (Rhesus monkey). We also compared the use of the carriers demineralized freeze-dried bone allograft (DFDBA) and coralline hydroxyapatite (CHA) for applying rhBMP-2. After computed tomography of the monkey head, custom meshes were made, loaded with rhBMP-2-incorporated DFDBA or CHA, and implanted in the latissimus dorsi muscle. Meanwhile, contralateral segmental mandibular defects were created, and custom meshes loaded with DFDBA, CHA, or rhBMP-2-incooperated DFDBA and CHA were implanted in situ. Thirteen weeks later, the bone flaps with rhBMP-2-incorporated DFDBA or CHA were transferred to repair segmental mandibular defects. The meshes loaded with DFDBA or CHA alone showed no bone regeneration 13 weeks after implantation in latissimus dorsi muscle. Radiography, angiography and histological analysis were used to evaluate the repair and vascularization of the implant. Segmental mandibular defects were successfully restored with prefabricated bone flaps and rhBMP-2-incorporated CHA in situ, but other segmental mandibular defects remained with rhBMP-2-incorporated DFDBA, DFDBA and CHA in situ. Moreover, mandibles reconstructed with rhBMP-2-incorporated CHA bone flaps revealed more regenerated and homogeneous bone formation than did other reconstructions. The study suggested that the prefabrication technique induced better mandibular reconstruction and bone regeneration in quantity and quality. © 2010 Elsevier Ltd.en_HK
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterialsen_HK
dc.relation.ispartofBiomaterialsen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsBiomaterials. Copyright © Elsevier BV.-
dc.subjectCoralline hydroxyapatiteen_HK
dc.subjectDemineralized freeze-dried bone allograften_HK
dc.subjectMandibular reconstructionen_HK
dc.subjectPrefabricated vascularized bone flapsen_HK
dc.subjectRhBMP-2en_HK
dc.subject.meshBone Morphogenetic Proteins - administration and dosage-
dc.subject.meshBone Regeneration-
dc.subject.meshBone Substitutes - chemistry-
dc.subject.meshMandible - pathology - radiography - surgery-
dc.subject.meshRecombinant Proteins - administration and dosage-
dc.titlePrimate mandibular reconstruction with prefabricated, vascularized tissue-engineered bone flaps and recombinant human bone morphogenetic protein-2 implanted in situen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0142-9612&volume=31&issue=18&spage=4935&epage=4943&date=2010&atitle=Primate+mandibular+reconstruction+with+prefabricated,+vascularized+tissue-engineered+bone+flaps+and+recombinant+human+bone+morphogenetic+protein-2+implanted+in+situ-
dc.identifier.emailPeng, X: pengxin@hku.hken_HK
dc.identifier.authorityPeng, X=rp01370en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.biomaterials.2010.02.072en_HK
dc.identifier.pmid20346504-
dc.identifier.scopuseid_2-s2.0-77951974056en_HK
dc.identifier.hkuros169840-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77951974056&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume31en_HK
dc.identifier.issue18en_HK
dc.identifier.spage4935en_HK
dc.identifier.epage4943en_HK
dc.identifier.isiWOS:000277783100018-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridZhou, M=55277926000en_HK
dc.identifier.scopusauthoridPeng, X=35270121900en_HK
dc.identifier.scopusauthoridMao, C=35722690000en_HK
dc.identifier.scopusauthoridXu, F=35224238200en_HK
dc.identifier.scopusauthoridHu, M=26326877100en_HK
dc.identifier.scopusauthoridYu Guangyan, GY=36017878300en_HK
dc.identifier.citeulike6964432-

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