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Conference Paper: Bitter melon extracts suppressed differentiation and triglyceride content of 3T3-L1 adipocytes

TitleBitter melon extracts suppressed differentiation and triglyceride content of 3T3-L1 adipocytes
Authors
Issue Date2008
PublisherFederation of American Societies for Experimental Biology. Meeting abstracts can be accessed via http://www.fasebj.org/search.dtl
Citation
Experimental Biology 2008 - ASPET's Centennial Meeting, San Diego, CA, 5-9 April 2008. In The FASEB Journal, 2008, v. 22 n. 1S, p. 889.11 How to Cite?
AbstractBitter melon (BM; Momordica charantia) supplementation slows weight gain and tissue fat accumulation in fat diet-induced obese rat. Liver and muscle carnitine palmitoyl transferase-I expression was enhanced in BM rats, suggesting increased lipid oxidation. This study was designed to determine if extracts from BM juice can suppress triglyceride (TG) content in the 3T3-L1 cell line. 3T3-L1 pre-adipocytes were induced to differentiate in the presence or absence of an ethanol-water extract of BM juice. The latter was obtained by stepwise gradient elution of an organic extract of BM juice. Two fractions (eluted by 10% & 30% ethanol respectively and then freeze-dried; F2 at 350μg/mL & F3 at 50μg/mL) were found to reduce TG content on day 10 (p<0.01, F3) & day 12 (p<0.01, both); and GPDH activity on day 10 & 12 (p<0.01, both) of a 12-day study. Two-way ANOVA indicated reduced mRNA expression of fatty acid synthase (p<0.001), acetyl CoA carboxylase 1 (p<0.01) and Glut-4 (p<0.01) in BM-treated adipocytes. With F3, these effects primarily occurred between days 8–10. Downregulation of PPARγ (p<0.001) and SREBP-1c (p<0.001) mRNA expression was observed in F3-treated cells on day 12. The data suggest that bioactive ingredient(s) in BM may suppress TG synthesis in 3T3-L1 cells through suppressing the expression of certain lipogenic genes and transcription factors. (Supported by the Faculty Development Fund, HKU)
Persistent Identifierhttp://hdl.handle.net/10722/114878
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorTam, KSen_HK
dc.contributor.authorWu, Jen_HK
dc.contributor.authorTse, MYen_HK
dc.contributor.authorTam, TMen_HK
dc.contributor.authorWang, Men_HK
dc.contributor.authorLi, ETSen_HK
dc.date.accessioned2010-09-26T05:20:01Z-
dc.date.available2010-09-26T05:20:01Z-
dc.date.issued2008en_HK
dc.identifier.citationExperimental Biology 2008 - ASPET's Centennial Meeting, San Diego, CA, 5-9 April 2008. In The FASEB Journal, 2008, v. 22 n. 1S, p. 889.11-
dc.identifier.issn0892-6638-
dc.identifier.urihttp://hdl.handle.net/10722/114878-
dc.description.abstractBitter melon (BM; Momordica charantia) supplementation slows weight gain and tissue fat accumulation in fat diet-induced obese rat. Liver and muscle carnitine palmitoyl transferase-I expression was enhanced in BM rats, suggesting increased lipid oxidation. This study was designed to determine if extracts from BM juice can suppress triglyceride (TG) content in the 3T3-L1 cell line. 3T3-L1 pre-adipocytes were induced to differentiate in the presence or absence of an ethanol-water extract of BM juice. The latter was obtained by stepwise gradient elution of an organic extract of BM juice. Two fractions (eluted by 10% & 30% ethanol respectively and then freeze-dried; F2 at 350μg/mL & F3 at 50μg/mL) were found to reduce TG content on day 10 (p<0.01, F3) & day 12 (p<0.01, both); and GPDH activity on day 10 & 12 (p<0.01, both) of a 12-day study. Two-way ANOVA indicated reduced mRNA expression of fatty acid synthase (p<0.001), acetyl CoA carboxylase 1 (p<0.01) and Glut-4 (p<0.01) in BM-treated adipocytes. With F3, these effects primarily occurred between days 8–10. Downregulation of PPARγ (p<0.001) and SREBP-1c (p<0.001) mRNA expression was observed in F3-treated cells on day 12. The data suggest that bioactive ingredient(s) in BM may suppress TG synthesis in 3T3-L1 cells through suppressing the expression of certain lipogenic genes and transcription factors. (Supported by the Faculty Development Fund, HKU)-
dc.languageengen_HK
dc.publisherFederation of American Societies for Experimental Biology. Meeting abstracts can be accessed via http://www.fasebj.org/search.dtl-
dc.relation.ispartofThe FASEB Journalen_HK
dc.titleBitter melon extracts suppressed differentiation and triglyceride content of 3T3-L1 adipocytesen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailTse, MY: mytsea@hkusua.hku.hken_HK
dc.identifier.emailWang, M: mfwang@hkusua.hku.hken_HK
dc.identifier.emailLi, ETS: etsli@hkucc.hku.hken_HK
dc.identifier.authorityWang, M=rp00800en_HK
dc.identifier.authorityLi, ETS=rp00737en_HK
dc.identifier.hkuros150516en_HK

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