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Conference Paper: Development of 3-actoxymethoxycarbonyl-2,2,5,5-tetramethyl-1-pyrrolidinyloxyl as an electron paramagnetic resonance imaging reagent for in vivo mapping brain oxygen distribution and infarction in ischemic brain

TitleDevelopment of 3-actoxymethoxycarbonyl-2,2,5,5-tetramethyl-1-pyrrolidinyloxyl as an electron paramagnetic resonance imaging reagent for in vivo mapping brain oxygen distribution and infarction in ischemic brain
Authors
KeywordsBiology
Biophysics chemistry
Crystallography biology
Biochemistry
Issue Date2009
PublisherBiophysical Society of China.
Citation
The 6th Annual World Congress of International Brain Mapping & Intraoperative Surgical Planning Society (IBMISPS 2009), Boston, MA., 26-29 August 2009. In Acta Biophysica Sinica, 2009, v. 2009 n. S1, p. 101, article no. S6-5 How to Cite?
AbstractMeasurement of oxygen concentration and distribution in brain is essential to understanding the pathophysiology of stroke. Although brain oxygen level is critical for brain tissue survival, in vivo no-invasive measurement of brain oxygen distribution and identification of infarction area remain technical challenge. Low-frequency electron paramagnetic resonance (EPR) spectroscopy with application of oxygen sensitive probe is an attractive imaging modality that can potentially map O2 concentration in the brain. We recently developed a paramagnetic probe, 3-actoxymethoxycarbonyl-2,2,5,5-tetramethyl-1-pyrrolidinyloxyl (AM-CTP), for brain oxygen measurement with EPR spectroscopy. In this study, we demonstrated that after intraperitoneal administration of AM-CTP to mice this nitroxide diffused across the blood brain barrier where, after hydrolysis, 3-carboxy-2,2,5,5-tetramethyl- pyrrolidinyloxyl was liberated and entrapped. The probe is sensitive to the changes of oxygen concentration. Pharmacokinetic and pharmacodynamic studies showed that the probe was relatively stable enough for brain imaging study. By using AM-CTP and low-frequency EPR instrument, we obtained EPR brain oxygen map, for the first time, in mice of ischemic stroke. Furthermore, by co-registration with magnetic resonance imaging, we demonstrated that the EPR imaging technology could be potentially used for identification of infarction area in ischemic brain. This finding suggests that EPRI with specifically designed nitroxides is a promising imaging modality for visualization brain O2 distribution, especially in an ischemic brain. We believe that this imaging method can be used for monitoring the effects of therapeutic intervention, aimed at enhancing brain O2 supply, so crucial in minimizing brain injury following stroke.
DescriptionScientific Session 6: Traumatic Brain Injury
Persistent Identifierhttp://hdl.handle.net/10722/114448
ISSN

 

DC FieldValueLanguage
dc.contributor.authorShen, Jen_HK
dc.contributor.authorRosen, GMen_HK
dc.contributor.authorLiu, KJen_HK
dc.date.accessioned2010-09-26T05:00:54Z-
dc.date.available2010-09-26T05:00:54Z-
dc.date.issued2009en_HK
dc.identifier.citationThe 6th Annual World Congress of International Brain Mapping & Intraoperative Surgical Planning Society (IBMISPS 2009), Boston, MA., 26-29 August 2009. In Acta Biophysica Sinica, 2009, v. 2009 n. S1, p. 101, article no. S6-5-
dc.identifier.issn1000-6737-
dc.identifier.urihttp://hdl.handle.net/10722/114448-
dc.descriptionScientific Session 6: Traumatic Brain Injury-
dc.description.abstractMeasurement of oxygen concentration and distribution in brain is essential to understanding the pathophysiology of stroke. Although brain oxygen level is critical for brain tissue survival, in vivo no-invasive measurement of brain oxygen distribution and identification of infarction area remain technical challenge. Low-frequency electron paramagnetic resonance (EPR) spectroscopy with application of oxygen sensitive probe is an attractive imaging modality that can potentially map O2 concentration in the brain. We recently developed a paramagnetic probe, 3-actoxymethoxycarbonyl-2,2,5,5-tetramethyl-1-pyrrolidinyloxyl (AM-CTP), for brain oxygen measurement with EPR spectroscopy. In this study, we demonstrated that after intraperitoneal administration of AM-CTP to mice this nitroxide diffused across the blood brain barrier where, after hydrolysis, 3-carboxy-2,2,5,5-tetramethyl- pyrrolidinyloxyl was liberated and entrapped. The probe is sensitive to the changes of oxygen concentration. Pharmacokinetic and pharmacodynamic studies showed that the probe was relatively stable enough for brain imaging study. By using AM-CTP and low-frequency EPR instrument, we obtained EPR brain oxygen map, for the first time, in mice of ischemic stroke. Furthermore, by co-registration with magnetic resonance imaging, we demonstrated that the EPR imaging technology could be potentially used for identification of infarction area in ischemic brain. This finding suggests that EPRI with specifically designed nitroxides is a promising imaging modality for visualization brain O2 distribution, especially in an ischemic brain. We believe that this imaging method can be used for monitoring the effects of therapeutic intervention, aimed at enhancing brain O2 supply, so crucial in minimizing brain injury following stroke.-
dc.languageengen_HK
dc.publisherBiophysical Society of China.-
dc.relation.ispartofActa Biophysica Sinicaen_HK
dc.subjectBiology-
dc.subjectBiophysics chemistry-
dc.subjectCrystallography biology-
dc.subjectBiochemistry-
dc.titleDevelopment of 3-actoxymethoxycarbonyl-2,2,5,5-tetramethyl-1-pyrrolidinyloxyl as an electron paramagnetic resonance imaging reagent for in vivo mapping brain oxygen distribution and infarction in ischemic brainen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1000-6737&volume=S1&spage=101, article no. S6&epage=5&date=2009&atitle=Development+of+3-actoxymethoxycarbonyl-2,2,5,5-tetramethyl-1-pyrrolidinyloxyl+as+an+electron+paramagnetic+resonance+imaging+reagent+for+in+vivo+mapping+brain+oxygen+distribution+and+infarction+in+ischemic+brain-
dc.identifier.emailShen, J: shenjg@hkucc.hku.hken_HK
dc.identifier.hkuros164006en_HK
dc.identifier.volume2009-
dc.identifier.issueS1-
dc.identifier.spage101, article no. S6-5-
dc.identifier.epage101, article no. S6-5-
dc.description.otherThe 6th Annual World Congress of International Brain Mapping & Intraoperative Surgical Planning Society (IBMISPS), Boston, MA., 26-29 August 2009. In Acta Biophysica Sinica, 2009, S1, p. 101, article no. S6-5-

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