File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
  • Find via Find It@HKUL
Supplementary

Conference Paper: Differential Inhibition of Hedyotis diffusa (HD) and Scutellaria barbata (SB) on human nasopharyngeal carcinoma cells

TitleDifferential Inhibition of Hedyotis diffusa (HD) and Scutellaria barbata (SB) on human nasopharyngeal carcinoma cells
Authors
Issue Date2008
PublisherAmerican Association for Cancer Research
Citation
AACR 99th Annual Meeting, San Diego, CA, 12-16 April 2008. In Cancer Research, 2008, v. 68 n. 9S, Abstract no. 3327 How to Cite?
AbstractThere are increasing interests and growing opportunities for different investigations to verify Traditional Chinese medicines (TCM) as a complementary therapy for cancers. TCM has a growing popularity as a less intensive and more natural approach to achieve health or improve quality of life. Hedyotis diffusa (HD) and Scutellaria barbata (SB) are two popularly used TCM with documented lethal activities on hepatoma, cervical, gastric and intestinal carcinoma. In this study, we aimed to explore the inhibitory mechanism of the HD and SB extracts on Nasopharyngeal Carcinoma Cells (NPC/CNE2), which is a prevalent cancer in Hong Kong and Southern China. We determined the Chinese Medicine formula with restrict pharmaceutical procedures and performed the chemical fingerprint with HPLC for assessing the quality consistency of SB and HD. At 2-hour drug incubation, the CNE2 cells were well-responsive to both extracts and a lethal dose of 50 (LD50) was achieved with HD (276 µg/mL) and SB (65µg/mL) using trypan blue exclusion assay. At LD10, early apoptotic cell death was triggered by both extracts (HD: 28µg/mL; SB: 3µg/mL); and at LD30, late apoptotic/necrotic cells were significantly increased to 80% (HD: 276µg/mL; SB: 13µg/mL) by Annexin-V-FLOUS staining under fluorescent microscopy. The cytotoxic effect of HD and SB on CNE2 cells was predominantly mediated by H2O2 released by both extracts demonstrated by PerXOquantTM Quantitative Peroxide Assay. DNA damage was evident at half hour post treatment for both extracts using comet assay. In order to verify H2O2 was the toxic species that induced DNA damage, catalase was used to scavenge the H2O2 upon co-incubation with the herb extracts on the CNE2 cells. In conclusion, both HD and SB extracts exhibited a potent growth inhibition on NPC/CNE2 cells due to the induction of DNA damage and apoptosis via the generation of H2O2; hence both herbs can be potential therapeutic interventions for nasopharyngeal carcinoma.
Persistent Identifierhttp://hdl.handle.net/10722/114391
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372

 

DC FieldValueLanguage
dc.contributor.authorChu, ESen_HK
dc.contributor.authorSze, CWen_HK
dc.contributor.authorTong, Yen_HK
dc.contributor.authorWong, TKen_HK
dc.contributor.authorYow, CMen_HK
dc.date.accessioned2010-09-26T04:58:17Z-
dc.date.available2010-09-26T04:58:17Z-
dc.date.issued2008en_HK
dc.identifier.citationAACR 99th Annual Meeting, San Diego, CA, 12-16 April 2008. In Cancer Research, 2008, v. 68 n. 9S, Abstract no. 3327-
dc.identifier.issn0008-5472-
dc.identifier.urihttp://hdl.handle.net/10722/114391-
dc.description.abstractThere are increasing interests and growing opportunities for different investigations to verify Traditional Chinese medicines (TCM) as a complementary therapy for cancers. TCM has a growing popularity as a less intensive and more natural approach to achieve health or improve quality of life. Hedyotis diffusa (HD) and Scutellaria barbata (SB) are two popularly used TCM with documented lethal activities on hepatoma, cervical, gastric and intestinal carcinoma. In this study, we aimed to explore the inhibitory mechanism of the HD and SB extracts on Nasopharyngeal Carcinoma Cells (NPC/CNE2), which is a prevalent cancer in Hong Kong and Southern China. We determined the Chinese Medicine formula with restrict pharmaceutical procedures and performed the chemical fingerprint with HPLC for assessing the quality consistency of SB and HD. At 2-hour drug incubation, the CNE2 cells were well-responsive to both extracts and a lethal dose of 50 (LD50) was achieved with HD (276 µg/mL) and SB (65µg/mL) using trypan blue exclusion assay. At LD10, early apoptotic cell death was triggered by both extracts (HD: 28µg/mL; SB: 3µg/mL); and at LD30, late apoptotic/necrotic cells were significantly increased to 80% (HD: 276µg/mL; SB: 13µg/mL) by Annexin-V-FLOUS staining under fluorescent microscopy. The cytotoxic effect of HD and SB on CNE2 cells was predominantly mediated by H2O2 released by both extracts demonstrated by PerXOquantTM Quantitative Peroxide Assay. DNA damage was evident at half hour post treatment for both extracts using comet assay. In order to verify H2O2 was the toxic species that induced DNA damage, catalase was used to scavenge the H2O2 upon co-incubation with the herb extracts on the CNE2 cells. In conclusion, both HD and SB extracts exhibited a potent growth inhibition on NPC/CNE2 cells due to the induction of DNA damage and apoptosis via the generation of H2O2; hence both herbs can be potential therapeutic interventions for nasopharyngeal carcinoma.-
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research-
dc.relation.ispartofCancer Researchen_HK
dc.titleDifferential Inhibition of Hedyotis diffusa (HD) and Scutellaria barbata (SB) on human nasopharyngeal carcinoma cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailSze, CW: stephens@hku.hken_HK
dc.identifier.emailTong, Y: tongyao@hkucc.hku.hken_HK
dc.identifier.authorityTong, Y=rp00509en_HK
dc.identifier.hkuros141899en_HK
dc.identifier.volume68en_HK
dc.identifier.spage30en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats