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Conference Paper: Fertility study of complement-3 in mice

TitleFertility study of complement-3 in mice
Authors
Issue Date2006
Citation
The 37th Annual Scientific Meeting of the Society for Reproductive Biology (SRB 2006), Gold Coast, Australia., 20-23 August 2006. In Conference Proceedings, 2006, p. 92, abstract no. 304 How to Cite?
AbstractHuman oviductal cells produce complement-3 (C3) and its embryotrophic derivative, iC3b in the presence of embryos. We proposed that C3-deficiency would lead to fertility impairment in mice in vivo. To determine the physiological significance of this protein in reproduction, heterozygous mice carrying the mutated C3 gene (C57BL/6J-C3tmlCrr) were purchased from Jackson Laboratory. Crossing of these mice (C3+/-) generated mice carrying the homozygous (C3+/+) and mutated C3 (C3-/-) genes. C3-/- and the wild type C3+/+ were allowed to cage with males of the same genotype for 6 months and their fertility was examined. Both genotypes are fertile and produce viable pups. The number of litters per week born from C3-/- pairs (0.116±0.05) were significantly smaller than those in C3 +/+ pairs (0.168±0.04). There were no significant difference between the mean numbers of pups per litter, mean born weight and mean litter size at wean between the two groups. However, the mean pup weight at weaning of C3 -/- pairs (8.1± 1.2 g .) was significantly smaller than that of C3 +/+ pairs (8.6±1.3 g). Although C3 protein could not be detected in the C3 -/- mice serum by Western blot, C3 immunoreactivity and mRNA was detected in the oviduct and liver tissues homogenate, suggesting the presence of mutated C3 molecules in these animals. These mating results suggested that the C3 -/- mice require longer getting pregnant and the resulting pups are smaller in size at weaning. The biological activity of the mutated C3 molecule on embryo development remains to be investigated.
DescriptionSession: SRB Orals - Embryo Development
Persistent Identifierhttp://hdl.handle.net/10722/113878

 

DC FieldValueLanguage
dc.contributor.authorChow, WNen_HK
dc.contributor.authorLee, CYLen_HK
dc.contributor.authorWong, BPCen_HK
dc.contributor.authorChung, MKen_HK
dc.contributor.authorTse, PKen_HK
dc.contributor.authorLee, CKFen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-26T04:35:09Z-
dc.date.available2010-09-26T04:35:09Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 37th Annual Scientific Meeting of the Society for Reproductive Biology (SRB 2006), Gold Coast, Australia., 20-23 August 2006. In Conference Proceedings, 2006, p. 92, abstract no. 304en_HK
dc.identifier.urihttp://hdl.handle.net/10722/113878-
dc.descriptionSession: SRB Orals - Embryo Development-
dc.description.abstractHuman oviductal cells produce complement-3 (C3) and its embryotrophic derivative, iC3b in the presence of embryos. We proposed that C3-deficiency would lead to fertility impairment in mice in vivo. To determine the physiological significance of this protein in reproduction, heterozygous mice carrying the mutated C3 gene (C57BL/6J-C3tmlCrr) were purchased from Jackson Laboratory. Crossing of these mice (C3+/-) generated mice carrying the homozygous (C3+/+) and mutated C3 (C3-/-) genes. C3-/- and the wild type C3+/+ were allowed to cage with males of the same genotype for 6 months and their fertility was examined. Both genotypes are fertile and produce viable pups. The number of litters per week born from C3-/- pairs (0.116±0.05) were significantly smaller than those in C3 +/+ pairs (0.168±0.04). There were no significant difference between the mean numbers of pups per litter, mean born weight and mean litter size at wean between the two groups. However, the mean pup weight at weaning of C3 -/- pairs (8.1± 1.2 g .) was significantly smaller than that of C3 +/+ pairs (8.6±1.3 g). Although C3 protein could not be detected in the C3 -/- mice serum by Western blot, C3 immunoreactivity and mRNA was detected in the oviduct and liver tissues homogenate, suggesting the presence of mutated C3 molecules in these animals. These mating results suggested that the C3 -/- mice require longer getting pregnant and the resulting pups are smaller in size at weaning. The biological activity of the mutated C3 molecule on embryo development remains to be investigated.-
dc.languageengen_HK
dc.relation.ispartofAnnual Scientific Meeting of the Society for Reproductive Biology, SRB 2006 Proceedingsen_HK
dc.titleFertility study of complement-3 in miceen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailChow, WN: cwn5810@gmail.comen_HK
dc.identifier.emailLee, CYL: cherielee@hkusua.hku.hken_HK
dc.identifier.emailWong, BPC: bpcwong@HKUCC.hku.hken_HK
dc.identifier.emailChung, MK: mkchung@ymail.comen_HK
dc.identifier.emailTse, PK: apbtnui@hkusua.hku.hken_HK
dc.identifier.emailLee, CKF: ckflee@hkucc.hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityLee, CYL=rp00308en_HK
dc.identifier.authorityLee, CKF=rp00458en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.hkuros127476en_HK
dc.identifier.spage304en_HK

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