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Conference Paper: Hormonal regulation and convertase activities of complement 3 in human oviductal epithelial cells

TitleHormonal regulation and convertase activities of complement 3 in human oviductal epithelial cells
Authors
Issue Date2006
Citation
The 37th Annual Scientific Meeting of the Society for Reproductive Biology (SRB 2006), Gold Coast, Australia., 20-23 August 2006. In Conference Proceedings, 2006, p. 65, abstract no. 245 How to Cite?
AbstractHuman oviduct cells produce complement 3 (C3). The derivative of C3, iC3b, but not C3 enhanced mouse preimplantation embryo development. We hypothesized that the human oviduct uses the complement pathways for complement activation to convert C3 to iC3b via C3b and that production of C3 in the oviducts is under hormonal regulation. The aim of this study is to investigate the effect of hormones on C3 mRNA expression and the conversion of C3 into C3b/iC3b in the human oviductal epithelial cells (OE). In vitro cultured primary OE cells were treated with varies concentrations of estrogen and progesterone either alone or in combination. Estrogen enhanced C3 mRNA expression of OE cells. Progesterone alone has no effect on C3 expression. The presence of the components of C3 convertases were studied by RT-PCR and immunocytochemistry, respectively. Molecules involved in complement activation, C2 and C4 in the classical pathway and lectin pathway, and factor B (fB) and factor D (fD) in the alternative pathway was detected in the OE cells. The OE cell culture possessed active C3 convertase that converted exogenous C3 into C3b in a time-dependent manner. No iC3b was produced under this condition. In conclusion, the production of C3 in oviduct is estrogen-regulated and the oviductal cells can convert C3 toC3b but not iC3b.
DescriptionSession: ESA / SRB Orals - Joint Female Reproduction
Persistent Identifierhttp://hdl.handle.net/10722/113877

 

DC FieldValueLanguage
dc.contributor.authorLee, CYLen_HK
dc.contributor.authorTse, PKen_HK
dc.contributor.authorChow, WNen_HK
dc.contributor.authorLee, CKFen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-26T04:35:07Z-
dc.date.available2010-09-26T04:35:07Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 37th Annual Scientific Meeting of the Society for Reproductive Biology (SRB 2006), Gold Coast, Australia., 20-23 August 2006. In Conference Proceedings, 2006, p. 65, abstract no. 245en_HK
dc.identifier.urihttp://hdl.handle.net/10722/113877-
dc.descriptionSession: ESA / SRB Orals - Joint Female Reproduction-
dc.description.abstractHuman oviduct cells produce complement 3 (C3). The derivative of C3, iC3b, but not C3 enhanced mouse preimplantation embryo development. We hypothesized that the human oviduct uses the complement pathways for complement activation to convert C3 to iC3b via C3b and that production of C3 in the oviducts is under hormonal regulation. The aim of this study is to investigate the effect of hormones on C3 mRNA expression and the conversion of C3 into C3b/iC3b in the human oviductal epithelial cells (OE). In vitro cultured primary OE cells were treated with varies concentrations of estrogen and progesterone either alone or in combination. Estrogen enhanced C3 mRNA expression of OE cells. Progesterone alone has no effect on C3 expression. The presence of the components of C3 convertases were studied by RT-PCR and immunocytochemistry, respectively. Molecules involved in complement activation, C2 and C4 in the classical pathway and lectin pathway, and factor B (fB) and factor D (fD) in the alternative pathway was detected in the OE cells. The OE cell culture possessed active C3 convertase that converted exogenous C3 into C3b in a time-dependent manner. No iC3b was produced under this condition. In conclusion, the production of C3 in oviduct is estrogen-regulated and the oviductal cells can convert C3 toC3b but not iC3b.-
dc.languageengen_HK
dc.relation.ispartofAnnual Scientific Meeting of the Society for Reproductive Biology, SRB 2006 Proceedingsen_HK
dc.titleHormonal regulation and convertase activities of complement 3 in human oviductal epithelial cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLee, CYL: cherielee@hkusua.hku.hken_HK
dc.identifier.emailTse, PK: apbtnui@hkusua.hku.hken_HK
dc.identifier.emailChow, WN: cwn5810@gmail.comen_HK
dc.identifier.emailLee, CKF: ckflee@hkucc.hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityLee, CYL=rp00308en_HK
dc.identifier.authorityLee, CKF=rp00458en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.hkuros127477en_HK
dc.identifier.spage65, abstract no. 245en_HK
dc.identifier.epage65, abstract no. 245-

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