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Conference Paper: Characterization of a novel acrosome-expressing protein 2 (AEP2/VAD1.2) during spermatogenesis

TitleCharacterization of a novel acrosome-expressing protein 2 (AEP2/VAD1.2) during spermatogenesis
Authors
Issue Date2006
PublisherHong Kong Academy of Medicine
Citation
The 4th International Huaxia Congress of Endocrinology, Hong Kong, 15–18 December 2006. In Hong Kong Medical Journal, 2006, v. 12 n. 6 S4, p. 111 Abstract no. P88 How to Cite?
AbstractSpermatogenesis is a complex process in which undifferentiated male germ cells undergo mitotic and meiotic divisions, followed by a dramatic morphological reorganization to generate spermatozoa that are capable of fertilizing an oocyte. Maturation and differentiation of male germ cells take place within seminiferous tubules and the process occurs in a cyclic manner. Recently, we used vitamin A-deficiency (VAD) rat model to synchronize spermatogenesis in rat testis and mRNA differential display to profile the gene expression patterns in retinol re-initiated (PVA-VAD) spermatogenesis. Twelve cDNA fragments including VAD1.2 and VAD1.3 that are differentially expressed in testis were isolated. Objectives: To characterize the acrosome-expressing protein 2 (AEP2/VAD1.2). Methods and Results: AEP2 was expressed in mouse on postnatal Day 25 coincided with the formation of spermatids. The gene coding for AEP2 is located on chromosome 11E1 containing 5 exons. RT-PCR and Northern blot analysis suggested that AEP2 was highly expressed in the germ cell of mouse testis at stages X-XII. Immunohistochemistry and immunofluorescence colocalization studies revealed an acrosome-specific expression pattern in developing spermatids of rat, mouse, human, pig and monkey. Western blot analysis confirmed a protein band of size 40-kDa in both rat and mouse testis lysates. Conclusions: The specific temporal and spatial expression of AEP2 suggested that it may play important roles in the maturation and differentiation of spermatids during spermiogenesis. (This work is supported in part by a RCG grant HKU7357/05M to KFL.)
Persistent Identifierhttp://hdl.handle.net/10722/113817
ISSN
2015 Impact Factor: 0.887
2015 SCImago Journal Rankings: 0.279

 

DC FieldValueLanguage
dc.contributor.authorTam, YTen_HK
dc.contributor.authorZuo, Yen_HK
dc.contributor.authorLuk, JMCen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorLee, CKFen_HK
dc.date.accessioned2010-09-26T04:32:30Z-
dc.date.available2010-09-26T04:32:30Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 4th International Huaxia Congress of Endocrinology, Hong Kong, 15–18 December 2006. In Hong Kong Medical Journal, 2006, v. 12 n. 6 S4, p. 111 Abstract no. P88-
dc.identifier.issn1024-2708-
dc.identifier.urihttp://hdl.handle.net/10722/113817-
dc.description.abstractSpermatogenesis is a complex process in which undifferentiated male germ cells undergo mitotic and meiotic divisions, followed by a dramatic morphological reorganization to generate spermatozoa that are capable of fertilizing an oocyte. Maturation and differentiation of male germ cells take place within seminiferous tubules and the process occurs in a cyclic manner. Recently, we used vitamin A-deficiency (VAD) rat model to synchronize spermatogenesis in rat testis and mRNA differential display to profile the gene expression patterns in retinol re-initiated (PVA-VAD) spermatogenesis. Twelve cDNA fragments including VAD1.2 and VAD1.3 that are differentially expressed in testis were isolated. Objectives: To characterize the acrosome-expressing protein 2 (AEP2/VAD1.2). Methods and Results: AEP2 was expressed in mouse on postnatal Day 25 coincided with the formation of spermatids. The gene coding for AEP2 is located on chromosome 11E1 containing 5 exons. RT-PCR and Northern blot analysis suggested that AEP2 was highly expressed in the germ cell of mouse testis at stages X-XII. Immunohistochemistry and immunofluorescence colocalization studies revealed an acrosome-specific expression pattern in developing spermatids of rat, mouse, human, pig and monkey. Western blot analysis confirmed a protein band of size 40-kDa in both rat and mouse testis lysates. Conclusions: The specific temporal and spatial expression of AEP2 suggested that it may play important roles in the maturation and differentiation of spermatids during spermiogenesis. (This work is supported in part by a RCG grant HKU7357/05M to KFL.)-
dc.languageengen_HK
dc.publisherHong Kong Academy of Medicine-
dc.relation.ispartofHong Kong Medical Journalen_HK
dc.titleCharacterization of a novel acrosome-expressing protein 2 (AEP2/VAD1.2) during spermatogenesisen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailTam, YT: h0202957@hkusua.hku.hken_HK
dc.identifier.emailZuo, Y: ho494060@hkusua.hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailLee, CKF: ckflee@hkucc.hku.hken_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.authorityLee, CKF=rp00458en_HK
dc.identifier.hkuros134625en_HK

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