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Conference Paper: Distributions of alpha 2 adrenoceptor subtypes in porcine coronary vasculature

TitleDistributions of alpha 2 adrenoceptor subtypes in porcine coronary vasculature
Authors
Issue Date2008
PublisherFederation of American Societies for Experimental Biology. Meeting abstracts can be accessed via http://www.fasebj.org/search.dtl
Citation
Experimental Biology 2008 - ASPET's Centennial Meeting, San Diego, CA, 5-9 April 2008. In The FASEB Journal, 2008, v. 22 n. Meeting Abstract Supplement, p. 914.10 How to Cite?
AbstractAlpha 2-adrenoreceptor agonists affect vascular tone of coronary vasculature by multiple mechanisms. One of the mechanisms is the direct action on endothelial {alpha}2 adrenoreceptors (mainly {alpha}2A), which induces an endothelial-dependent vasorelaxation via release of nitric oxide. We sought to investigate the subtypes of {alpha}2 adrenoceptors and their relative abundance in coronary blood vessels of different sizes (arteries vs arterioles). Result of RT-PCR clearly demonstrated that mRNA of {alpha}2A and {alpha}2B adrenoceptors was present in coronary arteries but mRNA of {alpha}2C-adrenoceptors was absent. In contrast, mRNA of {alpha}2B and {alpha}2C adrenoceptors was present in subendocardial myocardium (which contains coronary arterioles) but mRNA of {alpha}2A adrenoceptors was not. While mRNA of {alpha}2B adrenoceptors were detected in both coronary arteries and subendocardial myocardium, quantitative analysis revealed that its expression was higher in coronary arteries. Immunohistochemical studies showed that {alpha}2A and {alpha}2B adrenoceptor proteins were located mainly on the endothelial cells of coronary arteries but none was visualized in coronary arterioles. Distributions and cellular localizations of {alpha}2C adrenoceptor proteins had not yet been studied since specific antibody was not available. This preliminary data suggests that coronary blood vessels of different sizes may respond differently to pharmacological agents.
Persistent Identifierhttp://hdl.handle.net/10722/112812
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorHo, YWen_HK
dc.contributor.authorLeung, GPHen_HK
dc.contributor.authorMan, RYKen_HK
dc.contributor.authorVanhoutte, PMGRen_HK
dc.contributor.authorNg, JKFen_HK
dc.date.accessioned2010-09-26T03:48:18Z-
dc.date.available2010-09-26T03:48:18Z-
dc.date.issued2008en_HK
dc.identifier.citationExperimental Biology 2008 - ASPET's Centennial Meeting, San Diego, CA, 5-9 April 2008. In The FASEB Journal, 2008, v. 22 n. Meeting Abstract Supplement, p. 914.10-
dc.identifier.issn0892-6638-
dc.identifier.urihttp://hdl.handle.net/10722/112812-
dc.description.abstractAlpha 2-adrenoreceptor agonists affect vascular tone of coronary vasculature by multiple mechanisms. One of the mechanisms is the direct action on endothelial {alpha}2 adrenoreceptors (mainly {alpha}2A), which induces an endothelial-dependent vasorelaxation via release of nitric oxide. We sought to investigate the subtypes of {alpha}2 adrenoceptors and their relative abundance in coronary blood vessels of different sizes (arteries vs arterioles). Result of RT-PCR clearly demonstrated that mRNA of {alpha}2A and {alpha}2B adrenoceptors was present in coronary arteries but mRNA of {alpha}2C-adrenoceptors was absent. In contrast, mRNA of {alpha}2B and {alpha}2C adrenoceptors was present in subendocardial myocardium (which contains coronary arterioles) but mRNA of {alpha}2A adrenoceptors was not. While mRNA of {alpha}2B adrenoceptors were detected in both coronary arteries and subendocardial myocardium, quantitative analysis revealed that its expression was higher in coronary arteries. Immunohistochemical studies showed that {alpha}2A and {alpha}2B adrenoceptor proteins were located mainly on the endothelial cells of coronary arteries but none was visualized in coronary arterioles. Distributions and cellular localizations of {alpha}2C adrenoceptor proteins had not yet been studied since specific antibody was not available. This preliminary data suggests that coronary blood vessels of different sizes may respond differently to pharmacological agents.-
dc.languageengen_HK
dc.publisherFederation of American Societies for Experimental Biology. Meeting abstracts can be accessed via http://www.fasebj.org/search.dtlen_HK
dc.relation.ispartofThe FASEB Journalen_HK
dc.titleDistributions of alpha 2 adrenoceptor subtypes in porcine coronary vasculatureen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0176-8638&volume=&spage=&epage=&date=2008&atitle=Distributions+of+alpha+2+adrenoceptor+subtypes+in+porcine+coronary+vasculature.++San+Diego,+5-9+Apr+en_HK
dc.identifier.emailHo, YW: eywho@graduate.hku.hken_HK
dc.identifier.emailLeung, GPH: leung_pak_heng@hotmail.comen_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.emailVanhoutte, PMGR: vanhoutt@hku.hken_HK
dc.identifier.emailNg, JKF: jkfng@hkucc.hku.hken_HK
dc.identifier.authorityLeung, GPH=rp00234en_HK
dc.identifier.authorityMan, RYK=rp00236en_HK
dc.identifier.authorityNg, JKF=rp00544en_HK
dc.identifier.hkuros168525en_HK
dc.identifier.hkuros143139-

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