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Conference Paper: The role of repressor element 1 silencing transcription factor (REST) in modulating the transcription of human secretin receptor gene

TitleThe role of repressor element 1 silencing transcription factor (REST) in modulating the transcription of human secretin receptor gene
Authors
Issue Date2006
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/regpep
Citation
The 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France, 11-14 September 2005. In Regulatory Peptides, 2005, v. 130 n. 3, p. 167-168 How to Cite?
AbstractRecently, we have characterized the human secretin receptor (hSR) gene and demonstrated that the hSR promoter (-263 to -158, relative to the ATG start codon) is controlled by the ratio of activator Sp1 to repressor Sp3 competitively interacting with two GC-boxes. In the present study, a repressor-like element was identified at the 3V region (-101 to -46) of the minimal promoter. Scanning mutations within this region revealed a putative repressor element 1 (RE-1) binding motif. RE-1, also known as neuron-restrictive silencer element, is a 21-bp long, negative-acting DNA regulatory element. RE-1 interacts with a transcription factor, namely RE-1 silencing transcription factor (REST). REST represses gene expression by two repressor domains which recruit mSin3 and CoREST. RE-1 has been identified in an increasing number of neuronal genes, such as type II sodium channel, mu opioid receptor, and GABAA receptor g2. Recently, functional REST sites have also been discovered in VIP and PACAP genes. These observations are consistent with the scanning mutation analysis indicating a relation between RE-1 and hSR gene expression. Competitive gel mobility shift and supershift studies revealed the binding of REST with the RE-1 motif. Over-expression of REST in a REST non-expressing cells, PC12, dramatically decreased the RE-1 containing hSR promoter activity. Consistent with these results, the promoter activity and the transcript level of hSR are also augmented in Panc-1 cells after siRNA knock-down of REST. In summary, hSR gene expression is negatively regulated by REST through the RE-1 motif.
Descriptionpp. 133-188 of this journal issue entitled: Abstracts of the 7th International Symposium on VIP, PACAP and Related Peptides. Rouen, France. September 11-14, 2005
Persistent Identifierhttp://hdl.handle.net/10722/110593
ISSN
2015 Impact Factor: 1.813
2015 SCImago Journal Rankings: 0.915

 

DC FieldValueLanguage
dc.contributor.authorLee, TOen_HK
dc.contributor.authorLee, HYVen_HK
dc.contributor.authorYuen, PYen_HK
dc.contributor.authorChow, BKCen_HK
dc.date.accessioned2010-09-26T02:12:35Z-
dc.date.available2010-09-26T02:12:35Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France, 11-14 September 2005. In Regulatory Peptides, 2005, v. 130 n. 3, p. 167-168en_HK
dc.identifier.issn0167-0115en_HK
dc.identifier.urihttp://hdl.handle.net/10722/110593-
dc.descriptionpp. 133-188 of this journal issue entitled: Abstracts of the 7th International Symposium on VIP, PACAP and Related Peptides. Rouen, France. September 11-14, 2005-
dc.description.abstractRecently, we have characterized the human secretin receptor (hSR) gene and demonstrated that the hSR promoter (-263 to -158, relative to the ATG start codon) is controlled by the ratio of activator Sp1 to repressor Sp3 competitively interacting with two GC-boxes. In the present study, a repressor-like element was identified at the 3V region (-101 to -46) of the minimal promoter. Scanning mutations within this region revealed a putative repressor element 1 (RE-1) binding motif. RE-1, also known as neuron-restrictive silencer element, is a 21-bp long, negative-acting DNA regulatory element. RE-1 interacts with a transcription factor, namely RE-1 silencing transcription factor (REST). REST represses gene expression by two repressor domains which recruit mSin3 and CoREST. RE-1 has been identified in an increasing number of neuronal genes, such as type II sodium channel, mu opioid receptor, and GABAA receptor g2. Recently, functional REST sites have also been discovered in VIP and PACAP genes. These observations are consistent with the scanning mutation analysis indicating a relation between RE-1 and hSR gene expression. Competitive gel mobility shift and supershift studies revealed the binding of REST with the RE-1 motif. Over-expression of REST in a REST non-expressing cells, PC12, dramatically decreased the RE-1 containing hSR promoter activity. Consistent with these results, the promoter activity and the transcript level of hSR are also augmented in Panc-1 cells after siRNA knock-down of REST. In summary, hSR gene expression is negatively regulated by REST through the RE-1 motif.-
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/regpepen_HK
dc.relation.ispartofRegulatory Peptidesen_HK
dc.rightsRegulatory Peptides. Copyright © Elsevier BV.en_HK
dc.titleThe role of repressor element 1 silencing transcription factor (REST) in modulating the transcription of human secretin receptor geneen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0167-0115&volume=130&issue=3&spage=167&epage=168&date=2006&atitle=The+role+of+repressor+element+1+silencing+transcription+factor+(REST)+in+modulating+the+transcription+of+human+secretin+receptor+geneen_HK
dc.identifier.emailLee, TO: ltolee2@hkucc.hku.hken_HK
dc.identifier.emailChow, BKC: bkcc@hkusua.hku.hken_HK
dc.identifier.authorityLee, TO=rp00727en_HK
dc.identifier.authorityChow, BKC=rp00681en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.regpep.2005.06.011-
dc.identifier.hkuros103377en_HK
dc.identifier.hkuros123548-
dc.identifier.volume130en_HK
dc.identifier.issue3en_HK
dc.identifier.spage167en_HK
dc.identifier.epage168en_HK

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