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Conference Paper: Mitogenic effect of estrogen in human ovarian surface epithelial cells (OSE) involves downregulation of pigment epithelium-derived factor (PEDF)

TitleMitogenic effect of estrogen in human ovarian surface epithelial cells (OSE) involves downregulation of pigment epithelium-derived factor (PEDF)
Authors
Issue Date2006
PublisherHong Kong Medical Association. The Journal's web site is located at http://www.hkmj.org/
Citation
The 4th International Huaxia Congress of Endocrinology, Hong Kong, China, 15-18 December 2006, In Hong Kong Medical Journal, 2006, v. 12, suppl. 4, p. 171, abstract no. P215 How to Cite?
AbstractObjectives: To characterize the regulation of PEDF expression in OSE and its role in estrogen-dependent development of ovarian cancer derived from OSE. Methods: PEDF expression was examined in OSE cell lines and ovarian tumor tissues using immunohistochemistry, real-time PCR and Western blot. Cell proliferation and apoptosis were determined by MTT assay, TUNEL and Hoechst staining. Results: PEDF immunoreactivity was lower in a majority of ovarian tumors when compared with normal tissues. In cultured human OSE, exogenous PEDF caused significant growth inhibition and apoptosis. In line with the estrogen dependency of OSE-derived ovarian tumors, we showed for the first time that estrogen-induced OSE proliferation was accompanied by a specific inhibition of PEDF expression, which could be reversed by an estrogen receptor antagonist (ICI 182,780). Addition of exogenous PEDF antagonized the growth stimulatory effect of E2 on these cells, suggesting a negative role of PEDF in estrogen-induced OSE cell proliferation. Conclusion: This study is the first to identify PEDF as an ER-regulated target gene, and apart from its known function as a potent anti-angiogenic factor, is also implicated as a suppressor of estrogen-dependent ovarian tumor growth.
Persistent Identifierhttp://hdl.handle.net/10722/110508
ISSN
2015 Impact Factor: 0.887
2015 SCImago Journal Rankings: 0.279

 

DC FieldValueLanguage
dc.contributor.authorCheung, WTen_HK
dc.contributor.authorAu, SCLen_HK
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorNgan, HYSen_HK
dc.contributor.authorWong, ASTen_HK
dc.date.accessioned2010-09-26T02:08:56Z-
dc.date.available2010-09-26T02:08:56Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 4th International Huaxia Congress of Endocrinology, Hong Kong, China, 15-18 December 2006, In Hong Kong Medical Journal, 2006, v. 12, suppl. 4, p. 171, abstract no. P215-
dc.identifier.issn1024-2708-
dc.identifier.urihttp://hdl.handle.net/10722/110508-
dc.description.abstractObjectives: To characterize the regulation of PEDF expression in OSE and its role in estrogen-dependent development of ovarian cancer derived from OSE. Methods: PEDF expression was examined in OSE cell lines and ovarian tumor tissues using immunohistochemistry, real-time PCR and Western blot. Cell proliferation and apoptosis were determined by MTT assay, TUNEL and Hoechst staining. Results: PEDF immunoreactivity was lower in a majority of ovarian tumors when compared with normal tissues. In cultured human OSE, exogenous PEDF caused significant growth inhibition and apoptosis. In line with the estrogen dependency of OSE-derived ovarian tumors, we showed for the first time that estrogen-induced OSE proliferation was accompanied by a specific inhibition of PEDF expression, which could be reversed by an estrogen receptor antagonist (ICI 182,780). Addition of exogenous PEDF antagonized the growth stimulatory effect of E2 on these cells, suggesting a negative role of PEDF in estrogen-induced OSE cell proliferation. Conclusion: This study is the first to identify PEDF as an ER-regulated target gene, and apart from its known function as a potent anti-angiogenic factor, is also implicated as a suppressor of estrogen-dependent ovarian tumor growth.-
dc.languageengen_HK
dc.publisherHong Kong Medical Association. The Journal's web site is located at http://www.hkmj.org/-
dc.relation.ispartofHong Kong Medical Journalen_HK
dc.titleMitogenic effect of estrogen in human ovarian surface epithelial cells (OSE) involves downregulation of pigment epithelium-derived factor (PEDF)en_HK
dc.typeConference_Paperen_HK
dc.identifier.emailWong, AST: awong1@hkucc.hku.hken_HK
dc.identifier.authorityWong, AST=rp00805en_HK
dc.identifier.hkuros130204en_HK
dc.identifier.volume12-
dc.identifier.issuesuppl. 4-
dc.identifier.spage171, abstract no. P215-
dc.identifier.epage171, abstract no. P215-

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