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Conference Paper: Suppression of Met expression by RNA interference inhibits the metastatic potential of nasopharyngeal carcinoma cells

TitleSuppression of Met expression by RNA interference inhibits the metastatic potential of nasopharyngeal carcinoma cells
Authors
Issue Date2006
PublisherHong Kong Academy of Medicine
Citation
The 4th International Huaxia Congress of Endocrinology, Hong Kong, 15–18 December 2006. In Hong Kong Medical Journal, 2006, v. 12 n. 6 S4, p. 171 Abstract no. P214 How to Cite?
AbstractObjective: To investigate the effects of Met overexpression on the invasive phenotype of nasopharyngeal carcinoma (NPC), and to assess downstream signaling pathways mediated by hepatocyte growth factor (HGF). Methods: The NPC cell line CNE-2 was used as an in vitro model to examine the influence of Met overexpression in regulating cell invasion and migration. Met expression was knockdown by using Met specific siRNA, cell invasion was examined by a Matrigel invasion assay and in vitro migration was measured by the transwell migration assay. Results: Met downregulation by siRNA reduced the invasion-motile response to HGF. Treatment of the cells with specific inhibitors against Akt (LY294002) and JNK (SP600125) or expression of their dominant negative forms abolished HGF-stimulated cell invasion, associated with downregulation of matrix metalloproteinases-9. Conversely, the inhibition by ERK1/2 and p38 MAPK had no effect. Conclusions: Met overexpression may play an important role in the progression and metastasis of NPC by activating the Akt and JNK pathways, and raise the possibility of their application for cancer therapy.
Persistent Identifierhttp://hdl.handle.net/10722/110480
ISSN
2015 Impact Factor: 0.887
2015 SCImago Journal Rankings: 0.279

 

DC FieldValueLanguage
dc.contributor.authorZhou, Hen_HK
dc.contributor.authorWan, KFen_HK
dc.contributor.authorWong, CKen_HK
dc.contributor.authorWong, AST-
dc.date.accessioned2010-09-26T02:07:44Z-
dc.date.available2010-09-26T02:07:44Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 4th International Huaxia Congress of Endocrinology, Hong Kong, 15–18 December 2006. In Hong Kong Medical Journal, 2006, v. 12 n. 6 S4, p. 171 Abstract no. P214-
dc.identifier.issn1024-2708-
dc.identifier.urihttp://hdl.handle.net/10722/110480-
dc.description.abstractObjective: To investigate the effects of Met overexpression on the invasive phenotype of nasopharyngeal carcinoma (NPC), and to assess downstream signaling pathways mediated by hepatocyte growth factor (HGF). Methods: The NPC cell line CNE-2 was used as an in vitro model to examine the influence of Met overexpression in regulating cell invasion and migration. Met expression was knockdown by using Met specific siRNA, cell invasion was examined by a Matrigel invasion assay and in vitro migration was measured by the transwell migration assay. Results: Met downregulation by siRNA reduced the invasion-motile response to HGF. Treatment of the cells with specific inhibitors against Akt (LY294002) and JNK (SP600125) or expression of their dominant negative forms abolished HGF-stimulated cell invasion, associated with downregulation of matrix metalloproteinases-9. Conversely, the inhibition by ERK1/2 and p38 MAPK had no effect. Conclusions: Met overexpression may play an important role in the progression and metastasis of NPC by activating the Akt and JNK pathways, and raise the possibility of their application for cancer therapy.-
dc.languageengen_HK
dc.publisherHong Kong Academy of Medicine-
dc.relation.ispartofHong Kong Medical Journalen_HK
dc.titleSuppression of Met expression by RNA interference inhibits the metastatic potential of nasopharyngeal carcinoma cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailWong, AST: awong1@hkucc.hku.hken_HK
dc.identifier.authorityWong, AST=rp00805en_HK
dc.identifier.hkuros130209en_HK

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