File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
  • Find via Find It@HKUL
Supplementary

Conference Paper: Sp1 and CREB promote nectin-2 transcription to regulate adherens junctions assembly in the testis

TitleSp1 and CREB promote nectin-2 transcription to regulate adherens junctions assembly in the testis
Authors
Issue Date2004
PublisherBioScientifica Ltd.
Citation
The 195th Meeting of the Society for Endocrinology joint with Diabetes UK and the Growth Factor Group, London, UK, 1-3 November 2004. In Endocrine Abstracts, 2004, v. 8, Abstract no. OC16 How to Cite?
AbstractNectin-2, a major protein component of the adherens junctions (AJs), is found between Sertoli cells and germ cells in the seminiferious epithelium. Recent studies have shown that the expression of nectin-2 gene in testis is crucial to maintain normal spermatogenesis since male knockout mice lacking nectin-2 gene are sterile and possess morphological abnormal spermatozoa. However, the molecular mechanisms governing its basal transcription remain poorly understood. By the use of Sertoli and germ cell-lines (TM4 and GC-2 Spd (ts) cells, respectively) in transient transfection studies, we showed that the minimal mouse nectin-2 promoter was located between nucleotides -316 and -211 relative to the translation start site. Two putative Specificity protein 1 (Sp1) motifs and one each of the cAMP response element (CRE), AP1 and AP2 motifs were identified within this region. Mutational studies showed that these two Sp1 motifs cooperated synergistically with the CRE motif, but not the AP1 and AP2 motifs, to regulate nectin-2 gene transcription in both TM4 and GC-2 Spd(ts) cells. By EMSAs, we found that an AP-1 consensus sequence was able to inhibit DNA-protein complex formation with the CRE/AP-1 motif, suggesting a cross-talk between the AP-1 transcription factor (c-Jun) and this CRE/AP-1 motif. Over-expressions of CRE-binding protein (CREB) and a serine-133-mutated CREB significantly increased the promoter activity, which suggests CREB to be one of crucial transcription factors involved in regulating nectin-2 gene transcription in a PKA-independent manner. It has been known that there is a cyclic expression of CREB throughout the spermatogenic cycle. Our present data provide new evidence that CREB regulates spermatogenesis by regulating nectin-2 gene transcription and controls the timely assembly and disassembly of AJs between Sertoli cells and spermatids. This work was supported by grants from the University of Hong Kong (CRCG) and the Hong Kong Research Grant Council (HKU 7194/01M).
Persistent Identifierhttp://hdl.handle.net/10722/110454
ISSN

 

DC FieldValueLanguage
dc.contributor.authorLui, WYen_HK
dc.contributor.authorSze, KLen_HK
dc.contributor.authorLee, WWMen_HK
dc.date.accessioned2010-09-26T02:06:37Z-
dc.date.available2010-09-26T02:06:37Z-
dc.date.issued2004en_HK
dc.identifier.citationThe 195th Meeting of the Society for Endocrinology joint with Diabetes UK and the Growth Factor Group, London, UK, 1-3 November 2004. In Endocrine Abstracts, 2004, v. 8, Abstract no. OC16-
dc.identifier.issn1470-3947-
dc.identifier.urihttp://hdl.handle.net/10722/110454-
dc.description.abstractNectin-2, a major protein component of the adherens junctions (AJs), is found between Sertoli cells and germ cells in the seminiferious epithelium. Recent studies have shown that the expression of nectin-2 gene in testis is crucial to maintain normal spermatogenesis since male knockout mice lacking nectin-2 gene are sterile and possess morphological abnormal spermatozoa. However, the molecular mechanisms governing its basal transcription remain poorly understood. By the use of Sertoli and germ cell-lines (TM4 and GC-2 Spd (ts) cells, respectively) in transient transfection studies, we showed that the minimal mouse nectin-2 promoter was located between nucleotides -316 and -211 relative to the translation start site. Two putative Specificity protein 1 (Sp1) motifs and one each of the cAMP response element (CRE), AP1 and AP2 motifs were identified within this region. Mutational studies showed that these two Sp1 motifs cooperated synergistically with the CRE motif, but not the AP1 and AP2 motifs, to regulate nectin-2 gene transcription in both TM4 and GC-2 Spd(ts) cells. By EMSAs, we found that an AP-1 consensus sequence was able to inhibit DNA-protein complex formation with the CRE/AP-1 motif, suggesting a cross-talk between the AP-1 transcription factor (c-Jun) and this CRE/AP-1 motif. Over-expressions of CRE-binding protein (CREB) and a serine-133-mutated CREB significantly increased the promoter activity, which suggests CREB to be one of crucial transcription factors involved in regulating nectin-2 gene transcription in a PKA-independent manner. It has been known that there is a cyclic expression of CREB throughout the spermatogenic cycle. Our present data provide new evidence that CREB regulates spermatogenesis by regulating nectin-2 gene transcription and controls the timely assembly and disassembly of AJs between Sertoli cells and spermatids. This work was supported by grants from the University of Hong Kong (CRCG) and the Hong Kong Research Grant Council (HKU 7194/01M).-
dc.languageengen_HK
dc.publisherBioScientifica Ltd.-
dc.relation.ispartofEndocrine Abstractsen_HK
dc.titleSp1 and CREB promote nectin-2 transcription to regulate adherens junctions assembly in the testisen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLui, WY: wylui@hku.hken_HK
dc.identifier.emailLee, WWM: hrszlwm@hku.hken_HK
dc.identifier.authorityLui, WY=rp00756en_HK
dc.identifier.authorityLee, WWM=rp00728en_HK
dc.identifier.hkuros100120en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats